Distillers' grain waste (DGW), the main waste in the Baijiu-making process, has been utilized as potential substrate for butanol production after an enzymatic hydrolysate process. However, large amounts of hexanoic acid contained in DGW enzymatic hydrolysate (DGWH) could significantly influence butanol fermentation. In this paper, the effect of hexanoic acid on butanol fermentation was studied, and the optimal detoxification methods under different hexanoic acid concentrations were explored by analytic hierarchy process. The results showed that hexanoic acid in DGWH could reach 0.47 g/L and the increase of hexanoic acid concentration would improve its inhibitory effect on butanol fermentation. Hexanoic acid above 0.2 g/L could result in the decrease of cell dry weight, sugar utilization, and the significant decrease of butanol and total solvent yield during fermentation. When the hexanoic acid content was below 0.4 g/L, butanol production of the biochar-treated DGWH was comparable to that of the control and the cost was relatively lower, which was considered as the optimal hexanoic acid detoxification method. When the hexanoic acid content reached 0.6 and 1.1 g/L, the removal rates of hexanoic acid by activated carbon were 80.0% and 76.3%, respectively, which were much higher than that of the anionic resin and biochar treatment, and was the best detoxification method. This study provides a reference for the comprehensive utilization of DGW to produce butanol.
XU Jian
,
FENG Junwei
,
HUANG Shuang
,
CHEN Runxi
,
HU Fang
,
WANG Zihao
,
ZHANG Ruijing
,
CAI Fengjiao
,
YU Qi
,
WANG Jiangbo
. Effect of hexanoic acid in distillers' grain waste hydrolysate on butanol fermentation and evaluation of detoxification strategy[J]. Food and Fermentation Industries, 2023
, 49(23)
: 89
-95
.
DOI: 10.13995/j.cnki.11-1802/ts.035242
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