Difference on composition and characteristic between two Maillard reaction products of hydrolysate derived from high-temperature sesame meal protein

  • LU Xin ,
  • ZHANG Lixia ,
  • SUN Qiang ,
  • GAO Jinhong ,
  • JIN Lu ,
  • HUANG Jinian
Expand
  • 1(Center of Agricultural and Sideline Products Processing of Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
    2(Henan Engineering Research Centre of Bioactive Substances in Agricultural Products, Zhengzhou 450002, China)
    3(Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)
    4(College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China)

Received date: 2023-01-19

  Revised date: 2023-03-03

  Online published: 2024-01-02

Abstract

To explore the variation rule for composition characteristics of raw material in various Maillard reaction systems, high temperature sesame meal protein hydrolysate (HTSPH) as a raw material, xylose, xylose and Cys were added respectively, after being reacted at 120 ℃ for 2 h, two kinds of Maillard reaction products were produced. The differences in volatile components, peptides, and amino acids between the raw material and products were detected using GC-MS, LC-MS, and LC, the variation in their apparent characteristics and antioxidant activity was also analyzed. Results showed that physicochemical properties of the two Maillard reaction products were remarkably different. The product derived from HTSPH and xylose had high browning intensity and strong roasted almond flavor, whose volatile components were mainly aldehyde, ketone, and heterocycle compounds; whereas, the product originated from HTSPH, xylose and Cys showed strong boiled meat flavor, whose volatile components contained abundant sulfur-containing substances. 383 peptides were destroyed and 386 peptides were produced throughout the reaction. Physicochemical differences in the peptides of raw material and products included hydrophobicity, hydrophilicity, and amphipathicity. The concentration of free amino acid decreased due to the reaction, and the addition of Cys drastically affected the composition of free amino acids in the two products. Both Maillard reaction products improved significantly in terms of their antioxidant activities when compared to HTSPH, but the antioxidant capacities of products lagged below Cys. The above results provide the data basis and theoretical reference for investigating the law for the participation of peptide and amino acid in the Maillard reaction, also offer a method reference for the utilization of high-temperature sesame meal.

Cite this article

LU Xin , ZHANG Lixia , SUN Qiang , GAO Jinhong , JIN Lu , HUANG Jinian . Difference on composition and characteristic between two Maillard reaction products of hydrolysate derived from high-temperature sesame meal protein[J]. Food and Fermentation Industries, 2023 , 49(23) : 156 -164 . DOI: 10.13995/j.cnki.11-1802/ts.034933

References

[1] 王瑞丹, 芦鑫, 孙强, 等.低强度亚临界水提取水溶性芝麻蛋白与多肽[J].中国油脂, 2021, 46(6):38-42;58.
WANG R D, LU X, SUN Q, et al.Extraction of water-soluble sesame protein and peptide by low strength subcritical water[J].China Oils and Fats, 2021, 46(6):38-42;58.
[2] 唐章晖. 芝麻饼粕的综合利用研究[D].长沙:湖南农业大学, 2013.
TANG Z H.The research on the integrated use of sesame Cake[D].Changsha:Hunan Agricultural University, 2013.
[3] 芦鑫, 孙强, 张丽霞, 等.亚临界水水解脱脂高温芝麻饼粕中蛋白与糖类研究[J].中国粮油学报, 2016, 31(10):66-72.
LU X, SUN Q, ZHANG L X, et al.Hydrolysis of protein and saccharide in high temperature defatted sesame meal by subcritical water[J].Journal of the Chinese Cereals and Oils Association, 2016, 31(10):66-72.
[4] SHEN Y, HU L T, XIA B, et al.Effects of different sulfur-containing substances on the structural and flavor properties of defatted sesame seed meal derived Maillard reaction products[J].Food Chemistry, 2021, 365:130463.
[5] 张国治, 袁东振, 芦鑫, 等.3种芝麻蛋白结构和性质比较研究[J].中国油脂, 2017, 42(7):55-58;64.
ZHANG G Z, YUAN D Z, LU X, et al.Comparison of structure and properties of three kinds of sesame proteins[J].China Oils and Fats, 2017, 42(7):55-58;64.
[6] 吴甜甜, 易军鹏, 董晶寅, 等.蒸汽爆破预处理对芝麻粕蛋白结构与功能特性的影响[J].食品与发酵工业, 2022, 48(23):194-200.
WU T T, YI J P, DONG J Y, et al.Effect of steam explosion pretreatment on the structure and functional properties of sesame meal protein[J].Food and Fermentation Industries, 2022, 48(23):194-200.
[7] CAI J S, ZHU Y Y, MA R H, et al.Effects of roasting level on physicochemical, sensory, and volatile profiles of soybeans using electronic nose and HS-SPME-GC-MS[J].Food Chemistry, 2021, 340:127880.
[8] 贾聪, 孟醒, 游静, 等.低苦味芝麻ACE抑制肽的制备及氨基酸组成和结构分析[J].食品与发酵工业, 2021, 47(17):172-178.
JIA C, MENG X, YOU J, et al.Preparation, amino acid composition and structure analysis of low bitterness sesame ACE inhibitory peptides[J].Food and Fermentation Industries, 2021, 47(17):172-178.
[9] WANG W L, ZHANG L, WANG Z Q, et al.Physicochemical and sensory variables of Maillard reaction products obtained from Takifugu obscurus muscle hydrolysates[J].Food Chemistry, 2019, 290:40-46.
[10] LI Y, ZHONG F, JI W, et al.Functional properties of Maillard reaction products of rice protein hydrolysates with mono-, oligo- and polysaccharides[J].Food Hydrocolloids, 2013, 30(1):53-60.
[11] 鲁伟, 任国谱, 宋俊梅.蛋白水解液中多肽含量的测定方法[J].食品科学, 2005, 26(7):169-171.
LU W, REN G P, SONG J M.Determination of content of peptides in protein hydrolysates[J].Food Science, 2005, 26(7):169-171.
[12] 涂云飞. 茚三酮法测定茶叶游离氨基酸总量研究[J].现代农业科技, 2018(14):235;238.
TU Y F.Determination of total free amino acid in tea by ninhydrin[J].Modern Agricultural Science and Technology, 2018(14):235;238.
[13] 芦鑫, 贾聪, 王瑞丹, 等.芝麻降压与抗氧化肽QCKH的构效关系、分子拼接及理化性质研究[J].食品与发酵工业, 2020, 46(19):70-77.
LU X, JIA C, WANG R D, et al.Study on structure-activity relationship, molecular docking and physicochemical properties of an ACE-inhibitory and antioxidant sesame peptide QCKH[J].Food and Fermentation Industries, 2020, 46(19):70-77.
[14] ZHANG Z Y, ELFALLEH W, HE S D, et al.Heating and cysteine effect on physicochemical and flavor properties of soybean peptide Maillard reaction products[J].International Journal of Biological Macromolecules, 2018, 120:2137-2146.
[15] LUO F, FEI X Q.Maillard reaction derived from oil-tea camellia seed through roasting[J].Journal of the Science of Food and Agriculture, 2019, 99(11):5000-5007.
[16] 王丹, 况丹妮, 刘若阳, 等.焦糖化与美拉德反应中DDMP、HMF及糠醛的生成研究[J].食品工业科技, 2022, 43(12):100-107.
WANG D, KUANG D N, LIU R Y, et al.Formation of DDMP, HMF and furfural in caramelization and Maillard reaction[J].Science and Technology of Food Industry, 2022, 43(12):100-107.
[17] JIN W G, DU Y N, PEI J J, et al.Characterization and antioxidant activity of Maillard reaction products from a scallop (Patinopecten yessoensis) gonad hydrolysates-sugar model system[J].Journal of Food Measurement and Characterization, 2018, 12(4):2883-2891.
[18] 徐永霞, 白旭婷, 冯媛, 等.基于GC-IMS和化学计量学分析海鲈鱼肉蒸制过程中风味物质的变化[J].食品科学, 2021, 42(22):270-275.
XU Y X, BAI X T, FENG Y, et al.Changes of flavor compounds in sea bass during steaming process as analyzed by gas chromatography-ion mobility spectroscopy and chemometrics[J].Food Science, 2021, 42(22):270-275.
[19] 赵健, 赵梦瑶, 曹长春, 等.脂肪醛对“半胱氨酸-木糖”美拉德反应进程及肉香物质形成的影响[J].食品科学, 2017, 38(20):63-69.
ZHAO J, ZHAO M Y, CAO C C, et al.Effect of aliphatic aldehydes on Maillard reaction between cysteine and xylose and meaty flavor formation[J].Food Science, 2017, 38(20):63-69.
[20] 陈金明, 杨盛茹, 张秋双, 等.麦胚蛋白水解物美拉德反应工艺优化及产物挥发性风味物质分析[J].食品安全质量检测学报, 2022, 13(16):5178-5186.
CHEN J M, YANG S R, ZHANG Q S, et al.Maillard reaction process optimization of wheat germ protein hydrolysate and analysis of volatile flavor compounds[J].Journal of Food Safety & Quality, 2022, 13(16):5178-5186.
[21] 袁军, 郝武斌, 杨肖, 等.氨基酸对牛肉酶解液美拉德反应产物风味的影响[J].中国食品添加剂, 2018(3):137-141.
YUAN J, HAO W B, YANG X, et al.The effect of amino acids on the flavors of products from Maillard reaction of beef enzymatic hydrolysate[J].China Food Additives, 2018(3):137-141.
[22] 刘建彬, 康乐, 刘梦娅, 等.鸡肉肽在美拉德反应中对生成肉味化合物的贡献研究[J].现代食品科技, 2015, 31(4):301-310.
LIU J B, KANG L, LIU M Y, et al.The contribution of chicken peptides towards the generation of meat flavor compounds via Maillard reaction[J].Modern Food Science and Technology, 2015, 31(4):301-310.
[23] POWELL T, BOWRA S, COOPER H J.Subcritical water processing of proteins:An alternative to enzymatic digestion?[J].Analytical Chemistry, 2016, 88(12):6425-6432.
[24] POWELL T, BOWRA S, COOPER H J.Subcritical water hydrolysis of peptides:Amino acid side-chain modifications[J].Journal of the American Society for Mass Spectrometry, 2017, 28(9):1775-1786.
[25] SUN A, WU W, SOLADOYE O P, et al.Maillard reaction of food-derived peptides as a potential route to generate meat flavor compounds:A review[J].Food Research International, 2022, 151:110823.
[26] ZOU T T, KANG L, YANG C, et al.Flavour precursor peptide from an enzymatic beef hydrolysate Maillard reaction-II:Mechanism of the synthesis of flavour compounds from a sulphur-containing peptide through a Maillard reaction[J].LWT, 2019, 110:8-18.
[27] NI Z J, WEI C K, ZHENG A R, et al.Analysis of key precursor peptides and flavor components of flaxseed derived Maillard reaction products based on iBAQ mass spectrometry and molecular sensory science[J].Food Chemistry:X, 2022, 13:100224.
[28] 钱敏, 白卫东, 赵文红, 等.不同氨基酸和糖对美拉德反应产物的影响[J].食品科学, 2016, 37(13):31-35.
QIAN M, BAI W D, ZHAO W H, et al.Effects of amino acids and reducing sugars on Maillard reaction products[J].Food Science, 2016, 37(13):31-35.
[29] PARLIMENT T H, MORELLO M, MCGORRIN R J.Thermally Generated Flavors:Maillard, Microwave, and Extrusion Processes[M].Washington DC.:American Chemical Society, 1993:158-179.
[30] 章银良, 蔡亚玲, 李鑫.美拉德反应产物功能特性应用研究进展[J].中国调味品, 2018, 43(1):195-200.
ZHANG Y L, CAI Y L, LI X.Research progress on application of functional properties of Maillard reaction products[J].China Condiment, 2018, 43(1):195-200.
Outlines

/