Food and Fermentation Industries >

2023 , Vol. 49 >Issue 23: 307 - 314

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034184

Chemical composition analysis of wax gourd pith based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry technology

  • LU Shengyong ,
  • FU Manqin ,
  • XU Yujuan ,
  • XIE Dasen ,
  • YU Yuanshan ,
  • WEN Jing ,
  • XIAO Gengsheng
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  • 1(Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China)
    2(Zhongkai College of Agricultural Engineering of Light Industry Food Institute, Guangzhou 510225, China)
    3(Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory fog New Technology Research of Vegetables, Guangzhou 510640, China)

Received date: 2022-11-02

  Revised date: 2022-11-23

  Online published: 2024-01-02

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Abstract

The metabolites from wax gourd pith were separated and identified by ultra-high performance liquid chromatography-tandem mass spectrometry-based extensive metabolomics technology and the information about the composition and abundance of metabolites in wax gourd pith was analyzed and described. By measuring the total flavonoids, total phenols, and antioxidant capacity of four parts of wax gourd skin, pulp, pith, and seed, it was found that the total flavone content of wax gourd pith was second only to that of wax gourd pulp, while the wax gourd pith had the highest total phenol content and the strongest antioxidant capacity. Therefore, the wax gourd pith was selected as the research object to analyze its chemical composition. Results showed that a total of 825 metabolites were isolated and identified from the pith of wax gourd, including 451 (59.01%) primary metabolites and 374 (40.99%) secondary metabolites, including amino acids and their derivatives (114, 21.41%), lipids (123, 13.28%), organic acids (74, 10.82%), sugars and alcohols (58, 6.68%), nucleotides and their derivatives (62, 5.62%), vitamins (20, 1.22%), phenolic acids (161, 21.10%), alkaloids (57, 7.84%), flavonoids (92, 7.36%), lignans and coumarins (51, 3.63%), and terpenes (13, 1.06%). The flesh of wax gourd was rich in various metabolites with high abundance and also had significant nutritional function, active effect and efficacy. The metabolites with relative content ≥0.5% had an important contribution to the nutrition and activity of wax gourd by-products. The results of this study provide a theoretical basis for the deep processing and utilization of wax gourd pulp by-products and improve the added value and economic benefits of the wax gourd industry.

Key words： wax gourd pith; widely targeted metabolomics; chemical composition; nutrients; functional activity

Cite this article

LU Shengyong , FU Manqin , XU Yujuan , XIE Dasen , YU Yuanshan , WEN Jing , XIAO Gengsheng . Chemical composition analysis of wax gourd pith based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry technology[J]. Food and Fermentation Industries, 2023 , 49(23) : 307 -314 . DOI: 10.13995/j.cnki.11-1802/ts.034184

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