Food and Fermentation Industries >

2023 , Vol. 49 >Issue 24: 70 - 79

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036768

Study on isoliquiritigenin nanoparticles for intervention of ulcerative colitis

  • ZHANG Linjing ,
  • QIAN Nan ,
  • YU Butao ,
  • CHEN Yuyao ,
  • MA Jiahui ,
  • ZHANG Chenxi ,
  • WANG Wei ,
  • CHENG Xiangrong
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Suzhou Institute for Food Control, Suzhou 215000, China)

Received date: 2023-07-13

  Revised date: 2023-08-23

  Online published: 2024-01-17

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Abstract

To improve the effect of isoliquiritigenin on ulcerative colitis (UC), zein-ISO nanoparticles were prepared by an anti-solvent precipitation method with zein and pectin as coating materials. The preparation conditions of isoliquiritigenin nanoparticles were optimized through single factor experiments and their physicochemical properties were characterized. The release behavior of ISO in the gastrointestinal tract before and after embedding was compared through simulated digestion experiments in vitro, and the intervention effect of zein-ISO on UC was evaluated through animal experiments. Results showed that the zein-ISO nanoparticles prepared with a mass concentration of 20 g/L of zein solution, a volume fraction of 80% of ethanol solution, an ISO addition of 6 mg, and a zein pectin mass ratio of 2∶1 had high stability with an average particle size of 102.3 nm and an embedding rate of 91.66%. The simulated digestion experiments in vitro showed that the release rate of zein-ISO in intestinal fluid (53.15%) was significantly higher than that in gastric fluid (18.85%). Animal experiments have shown that compared to free ISO, zein-ISO significantly improved the disease state of shortened colon length in colitis mice (P<0.001) and reduced the levels of inflammatory factors , including tumour necrosis factor-α, interleukin-1β, and interleukin-6 in colon tissue (P<0.001). The research results provide new ideas for the development and utilization of poorly water-soluble active dietary functional factors such as ISO, as well as intervention in UC.

Key words： isoliquiritigenin; zein; pectin; nanoparticles; ulcerative colitis

Cite this article

ZHANG Linjing , QIAN Nan , YU Butao , CHEN Yuyao , MA Jiahui , ZHANG Chenxi , WANG Wei , CHENG Xiangrong . Study on isoliquiritigenin nanoparticles for intervention of ulcerative colitis[J]. Food and Fermentation Industries, 2023 , 49(24) : 70 -79 . DOI: 10.13995/j.cnki.11-1802/ts.036768

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