To clarify the correlation between the antibacterial and antioxidant activities of shrimp-derived chitosan oligosaccharide and their average molecular weight, this study used ethanol fractional precipitation method to separate shrimp-derived chitosan hydrolysate, and obtained four chitosan oligosaccharide (COS50, COS60, COS70, COS70 s) with the average molecular weight of 2 365, 2 807, 3 105, 1 390 Da, respectively. The antibacterial and antioxidant activities of the four chitosan oligosaccharides were compared. It was found that COS50 (the average molecular weight of 2 362 Da) had strong antibacterial effects on Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas, and Shewanella, and the minimum inhibitory concentration (MIC) for the above-mentioned bacteria were 2.50, 1.25, 1.25, 0.62, 0.16 mg/mL, respectively. Overall, the antioxidant activity of chitosan oligosaccharides decreased with the increase of molecular weight. COS50 had the strongest ability to scavenge DPPH radicals with IC50 of 0.90 mg/mL. COS70s had the strongest total reducing power (equivalent to 9.87 milligram ascorbic acid per g), followed by COS50. Therefore, considering the antibacterial and antioxidant activities, COS50 (average molecular weight of 2 362 Da) was the optimal chitosan oligosaccharide. This study provides a theoretical reference for the development and application of shrimp-derived chitosan oligosaccharide.
LUO Wei
,
HUANG Xiaoyue
,
ZHONG Ping
,
CHEN Siqi
,
OU Jiahao
,
FANG Zhijia
,
DENG Qi
,
SUN Lijun
,
WANG Yaling
. Antibacterial and antioxidant activities of shrimp-derived chitosan oligosaccharides with different molecular weights[J]. Food and Fermentation Industries, 2023
, 49(24)
: 193
-197
.
DOI: 10.13995/j.cnki.11-1802/ts.033713
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