The purpose of this study was to screen His-Trp-His (HWH) and to prepare electrodes for the detection of Cd2+ by electrochemical method. The binding mode of metal ions (Cd2+, Cr2+, Cu2+, Pb2+, Ni2+, and Zn2+) with short peptide HWH was calculated by quantum chemical simulation. It was found that HWH and Cd2+ could form strong chelation, and the chelation distance was 2.0 Å, 2.1 Å, and 2.4 Å, respectively. The binding energy of Cd2+ to HWH was -6.4 kcal/mol. The preparation method of polypeptide electrodes was investigated. It was found that the best effect was obtained by the method of enriching chitosan and then adding polypeptide. The polypeptide electrode was used to detect a specific concentration of Cd2+ ion standard solution, and the electrolyte pH, enrichment time, and enrichment potential were investigated one by one by the principle of control variable method to find out the best detection conditions. Finally, the optimal conditions of the experiment were determined as the electrolyte of pH 6, enrichment time of 150 s, and enrichment potential of -1.1 V. A series of Cd2+ ionic solutions with concentration gradients showed that in the concentration range of 0.001-0.30 mg/L, the linear equation was I (μA)=86.78C (mg/L)+0.171 0, R2=0.998 9. After calculation, the detection limit of this method could reach 4.301×10-4 mg/L. The recovery rate of this method was 97.4%-103.5% for drinking water, lake water of a university city, and a certain beverage, which was similar to that of the atomic spectrum and inductively coupled plasma mass spectrometry. The experiment shows that the polypeptide electrode method can be used for the detection of Cd2+ ions.
GENG Chenggang
,
CHEN Wentao
,
GAO Huiwen
,
ZOU Ping
. Detection of Cd2+ by peptide His-Trp-His (HWH) modified electrode[J]. Food and Fermentation Industries, 2023
, 49(24)
: 275
-282
.
DOI: 10.13995/j.cnki.11-1802/ts.034693
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