Food and Fermentation Industries >

2023 , Vol. 49 >Issue 24: 302 - 311

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034855

Simultaneous determination of choline and L-carnitine in infant formula milk powder by ultra-performance liquid chromatography-tandem mass spectrometry-isotope labeling-acid hydrolysis

  • ZHAN Shengqun ,
  • GE Cheng ,
  • LIU Jinmei ,
  • DING Yuzhen ,
  • ZHOU Rongjie ,
  • LIU Gang ,
  • ZHANG Hao ,
  • ZHOU Jun
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  • (Ausnutria Dairy (China) Co.Ltd., Changsha 410200, China)

Received date: 2023-01-09

  Revised date: 2023-02-09

  Online published: 2024-01-17

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Abstract

An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of choline and L-carnitine in infant formula was established. The samples were extracted by hydrochloric acid hydrolysis, and impurity was removed by adjusting pH, then diluted with acetonitrile. In this method, the amide column was used, while acetonitrile and 10 mmol/L ammonium formate aqueous solution [formic acid pH adjusted to (5.0±0.1)] were the mobile phases, and the procedure of elution was gradient elution. The determination mode was MRM mode, quantified by the internal standard. In addition, the matrix effect was evaluated by the addition method after extraction. Results showed that the detection limits of choline and L-carnitine were 1 mg/kg and 0.1 mg/kg, respectively, and the quantitative limits were 3 mg/kg and 0.3 mg/kg. The linear ranged from 0.8 ng/mL to 200 ng/mL, the spike recovery ranged from 95% to 105%, and the results of the quality control samples were also well, in the characteristic value range. The precision of choline and L-carnitine were 1.77% and 4.36% for the instrument, and 2.37% and 2.71% for the method, respectively. The matrix effect investigation showed that L-carnitine had no matrix effect, while choline had a very significant matrix inhibition (P<0.01), so an internal standard correction was required. This method had low limits, simple pretreatment, and high accuracy, and it was suitable for the simultaneous determination of choline and L-carnitine in infant formula.

Key words： milk powder; isotope labelling; acid hydrolysis; ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); choline; L-carnitine

Cite this article

ZHAN Shengqun , GE Cheng , LIU Jinmei , DING Yuzhen , ZHOU Rongjie , LIU Gang , ZHANG Hao , ZHOU Jun . Simultaneous determination of choline and L-carnitine in infant formula milk powder by ultra-performance liquid chromatography-tandem mass spectrometry-isotope labeling-acid hydrolysis[J]. Food and Fermentation Industries, 2023 , 49(24) : 302 -311 . DOI: 10.13995/j.cnki.11-1802/ts.034855

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