This paper aimed to improve the utilization value of jujube and develop products for deep processing. This research examined the extraction method and application of cyclic adenosine monophosphate (cAMP) from jujube. Using a single-factor experiment, the effects of three parameters on the yield of cAMP were investigated, including solid-liquid ratio, extraction time, and ethanol concentration. The optimal conditions for each ingredient for the highest yield of cAMP were found, and the resulting extract was used to formulate a cream. Using the software Design-Expert V8.0.6 and a central combination design, the experimental group of response surfaces was created. The optimal extraction method for cAMP was determined by establishing a model based on the experimental data, which was 1∶25.42 (g∶mL) of the ratio of solids-liquids, 63.51 min of extraction time, and 61.63% of the concentration of ethanol, and the cream stability was evaluated. Under the optimal extraction conditions, the yield of cAMP was 189.07 μg/g, and a stable cream was formulated with the extract.
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