Food and Fermentation Industries >

2024 , Vol. 50 >Issue 2: 306 - 311

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035401

Determination of selenium species in selenium-enriched vegetables by high performance liquid chromatography-inductively coupled plasma-mass spectrometry after ultrasonic-assisted extraction with enzyme

  • LI Qian ,
  • LIU Xingyong ,
  • CHEN Xinglian ,
  • LUO Lan ,
  • YIN Benlin ,
  • LI Xinyu ,
  • DU Lijuan ,
  • WANG Yunmei
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  • 1(Institute of Agricultural Quality Standard & Testing Technique, Yunnan Academy of Agricultural Sciences, Kunming 650205, China)
    2(Yunnan Institute of Product Quality Supervision ＆Inspection, Kunming 650223, China)

Received date: 2023-03-07

  Revised date: 2023-03-24

  Online published: 2024-02-27

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Abstract

This study established a method to simultaneously detect the concentration of SeO2-4, SeO2-3, SeMet, SeCys2 and MeSeCys in selenium-enriched vegetables by high performance-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). Add 4 mg protease XIV and 5 mL aqueous solution with pH 7.5 in 0.2 g sample, treat with ultrasound at 37 ℃ for 80 min, the supernatant pass through 0.22 μm filter membrane. The five selenium species were separated completely within 7 min by Hamilton PRP-X100 anion exchange column under the condition of mobile phase 25 mmol/L citric acid (pH=6.0). The linear coefficients of five selenium species were between 0.999 3 to 0.999 5, and the recoveries of the spiked samples ranged from 96.3% to 104.2%, with the RSD below 7.6%. This method is simple, rapid, precision and accurate, it is thus suitable for the analysis of selenium species in selenium-enriched vegetables.

Key words： HPLC-ICP-MS; selenium species; selenium-enriched vegetables

Cite this article

LI Qian , LIU Xingyong , CHEN Xinglian , LUO Lan , YIN Benlin , LI Xinyu , DU Lijuan , WANG Yunmei . Determination of selenium species in selenium-enriched vegetables by high performance liquid chromatography-inductively coupled plasma-mass spectrometry after ultrasonic-assisted extraction with enzyme[J]. Food and Fermentation Industries, 2024 , 50(2) : 306 -311 . DOI: 10.13995/j.cnki.11-1802/ts.035401

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