Food and Fermentation Industries >

2024 , Vol. 50 >Issue 4: 77 - 84

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034524

Effects of different drying methods on in vitro antioxidant, anti-diabetic, and acetylcholinesterase inhibitory activities of polyphenol extracts from broken green tea

  • ZHANG Tipei ,
  • LI Qing ,
  • BAO Honghui ,
  • SHEN Zhengxin ,
  • SHI Juan ,
  • TANG Qianyong ,
  • CHENG Yifang ,
  • ZHOU Rui
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  • 1(International Scientific and Technological Cooperation Base for Research and Development of Traditional Medicine and Food Homology, Hubei University of Arts and Science, Xiangyang 441053, China)
    2(Xiangyang Academy of Agricultural Science, Xiangyang 441607, China)

Received date: 2022-12-05

  Revised date: 2023-01-05

  Online published: 2024-03-15

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Abstract

The in vitro antioxidant, anti-diabetic, and acetylcholinesterase inhibitory activities of polyphenol extracts from broken green tea under different drying treatments were investigated in this study. Two different drying processes, including steam drying combined with vacuum drying and vacuum-freeze drying, were used to dry the ethyl acetate extraction phase of broken green tea, the color values, scavenging abilities of DPPH free radicals, ABTS cationic radicals, and ·OH, FRAP antioxidant capacities, and the inhibitory activities against α-amylase, α-glucosidase, and acetylcholine esterase, as well as anti-glycosylation activities of two extracts, were comparatively evaluated. Results showed that the vacuum-freeze drying polyphenol extract from broken green tea (BGTE-FD) displayed a deeper yellow color and stronger scavenging abilities of DPPH free radicals, ABTS cationic radicals, and ·OH, and FRAP antioxidant capacities with the IC50 values of 2.64 mg/L, 5.17 mg/L, 247.01 mg/L, and 27.73 mg/L, respectively, and meanwhile exhibited higher inhibitory activities against α-amylase, acetylcholine esterase, and α-glucosidase with the IC50 values of 85.84 mg/L, 416.45 mg/L, and 4.38 mg/L, respectively, compared with the steam drying combined with vacuum drying polyphenol extract from broken green tea (BGTE-SVD). In addition, BGTE-FD showed stronger α-glucosidase inhibition than that of acarbose (IC50=929.59 mg/L). Moreover, the inhibition rates of BGTE-FD and BGTE-SVD on advanced glycosylation end products were 65.28% and 63.66%, respectively, slightly lower than that of aminoguanidine (71.81%). Therefore, the polyphenol extracted from broken green tea by vacuum-freeze drying method has strong in vitro antioxidant and anti-diabetic activity and certain inhibitory activity against acetylcholinesterase, and the broken green tea can be used as a potential natural resource for the development of hypoglycemic and anti-dementia products.

Key words： broken green tea; polyphenol extract; antioxidation activity; anti-diabetic activity; acetylcholinesterase inhibition

Cite this article

ZHANG Tipei , LI Qing , BAO Honghui , SHEN Zhengxin , SHI Juan , TANG Qianyong , CHENG Yifang , ZHOU Rui . Effects of different drying methods on in vitro antioxidant, anti-diabetic, and acetylcholinesterase inhibitory activities of polyphenol extracts from broken green tea[J]. Food and Fermentation Industries, 2024 , 50(4) : 77 -84 . DOI: 10.13995/j.cnki.11-1802/ts.034524

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