To investigate the effects of cold plasma (CP) on the structural properties of golden pompano (Trachinotus ovatus) myofibrillar protein (MP), the fish was treated with cold plasma with different voltage (10, 20, and 30 kV), the Ca2+-ATPase activity, myofibrillary fragmentation index (MFI), trichloroacetic acid (TCA) soluble peptide, total amino acid content, surface hydrophobicity, ultraviolet absorption spectrum, infrared spectrum, and SDS-PAGE of myofibrillar protein were measured after treatment. Results showed that the contents of MFI, TCA soluble peptide, and total amino acid content significantly increased (P<0.05), while Ca2+-ATPase activity significantly decreased with the increasing voltage of CP treatment, indicating that the degree of MP denaturation increased with increasing voltage. Meanwhile, the surface hydrophobicity and ultraviolet absorption intensity increased, however, there was no significant change in protein secondary structure and SDS-PAGE, indicating that high-voltage CP treatment could promote protein denaturation and a certain degree of tertiary structure stretching, but had little effect on the primary and secondary structures. Thus, it is necessary to select a suitable voltage CP to sterilize the fish, which could avoid the denaturation of aquatic proteins caused by excessive voltage.
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