Food and Fermentation Industries >

2024 , Vol. 50 >Issue 4: 191 - 196

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035715

DNA molecular identification of genuine medicinal material Cinnamomum cassia and Cinnamomum burmanni in Guangxi

  • LI Li ,
  • WU Guifan ,
  • LUO Yi ,
  • LI Lili ,
  • LING Jie ,
  • MA Shuangcheng
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  • 1(Guangxi Zhuang Autonomous Region Institute for Food and Drug Control, NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine, Nanning 530021, China)
    2(National Institutes for Food and Drug Control, Beijing 100050, China)

Received date: 2023-04-04

  Revised date: 2023-05-31

  Online published: 2024-03-15

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Abstract

This study aimed to establish an efficient and specific polymerase chain reaction PCR method for the identification of Cinnamomum cassia and Cinnamomum burmanni to ensure the safety and effectiveness of Cinnamomum cassia. By analyzing and comparing the psbA-trnH sequence differences of Cinnamomum cassia and Cinnamomum burmanni, specific single nucleotide polymorphism sites were found, and specific identification primers were designed. The samples of Cinnamomum cassia and Cinnamomum burmanni from different sources were specifically amplified by optimizing annealing temperature and cycle number and evaluating different polymerases and PCR thermal cycles, according to the size of specific amplification bands. When the annealing temperature was 54 ℃ and the cycle number was 35, a specific band of 100-200 bp was amplified in the Cinnamomum cassia with specific primers, while Cinnamomum burmanni has no specific band. When the annealing temperature was 56 ℃ and the cycle number was 40, a specific band of 200-300 bp was amplified in the Cinnamomum burmanni with specific primers, while Cinnamomum cassia had no specific band. The specific polymerase chain reaction method in this study can be used to quickly and accurately identify the Cinnamomum cassia and Cinnamomum burmanni and provide a reference for controlling the quality and security of Cinnamomum cassia.

Key words： Cinnamomum cassia; Cinnamomum burmanni; specific polymerase chain reaction; specific primers; quality and security

Cite this article

LI Li , WU Guifan , LUO Yi , LI Lili , LING Jie , MA Shuangcheng . DNA molecular identification of genuine medicinal material Cinnamomum cassia and Cinnamomum burmanni in Guangxi[J]. Food and Fermentation Industries, 2024 , 50(4) : 191 -196 . DOI: 10.13995/j.cnki.11-1802/ts.035715

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