High pressure homogenization was used to construct liposomes to improve the stability and bioaccessibility of coenzyme Q10 (CoQ10). Liposomes were prepared by ethanol injection method combined with high pressure homogenization technology, and their physicochemical indexes such as particle size, polydispersity index, potential, and encapsulation efficiency were analyzed, and the storage stability and simulated digestion characteristics of CoQ10 liposomes were evaluated. The result revealed that the prepared liposomes were spherical with the encapsulation efficiency of about 96%. The high pressure homogenization at 50 MPa and 100 MPa reduced the particle size of liposomes from (538.26±11.25) nm to (240.21±7.23) nm and (130.17±3.13) nm, respectively, without significant effect on the potential and encapsulation efficiency of liposomes. At the same time, high pressure homogenization treatment effectively delayed the increase of acid value and peroxide value of CoQ10 liposomes during storage, but too high homogenization pressure had an adverse effect on liposome stability. In vitro simulated digestion experiments showed that liposomes significantly increased the bioaccessibility of CoQ10, and high pressure homogenization increased the values to (31.97±1.62)% and (31.61±2.72)%, respectively. The experimental results show that the preparation of liposomes by high pressure homogenization technology is a reliable method to achieve high stability and high bioaccessibility delivery of CoQ10, which has reference value and guiding significance for the development of CoQ10-related products.
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