Quinic acid is a flavonoid of millet polyphenols.In this study, the H2O2-induced SH-SY5Y oxidative stress cell model was employed to explore the neuroprotective effect of quinic acid.The effect of quinic acid on the viability of SH-SY5Y cells was measured by the MTT method, and in the meanwhile, the activity of the antioxidant enzyme superoxide dismutase (SOD) and the level of the markers of oxidative stress, glutathione (GSH) and malondialdehyde (MDA), were measured.The expression of antioxidant enzymes and inflammatory factors was detected by real-time fluorescent quantitative PCR, and the protein level of inflammatory factors was determined by Western blot.Results showed that pre-treatment of quinic acid (100 μg/mL) on SH-SY5Y cells could significantly inhibit the decrease of cell survival rate induced by H2O2.The examination of antioxidant indices suggested that quinic acid treatment could upregulate the activity of SOD, increase the level of GSH, reduce that of MDA in SH-SY5Y cells, and upregulate the expression level of antioxidant factors, such as SOD, catalase (CAT), and glutathione peroxidase (GPx), to reduce oxidative damage.In addition, quinic acid could suppress the expression of inflammatory factors interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in SH-SY5Y cells induced by H2O2 to alleviate the damage of cells.In a word, quinic acid can protect neural cells from damage by inhibiting oxidative stress and exerting anti-inflammatory function.This study will contribute to the further exploration of the role of millet polyphenols in the prevention and treatment of oxidative stress-related diseases.
WANG Shuo
,
LIANG Ting
,
LUO Lei
,
LIU Yongqing
,
FANG Yong
,
LI Sen
,
GUAN Xiao
. Study on the antioxidant activity and neuroprotective effect of quinic acid[J]. Food and Fermentation Industries, 2024
, 50(5)
: 42
-47
.
DOI: 10.13995/j.cnki.11-1802/ts.034635
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