The objective of the study was to investigate the protective effect of wheat peptides on gastric mucosal injury caused by ethanol and its mechanism.After treatment with wheat peptides, ethanol induced gastric mucosal injury model in mice and human gastric mucosal epithelial cell (GES-1) injury model was used to observe the changes in body weight and gastric tissue damage and to assess the expression levels of oxidative stress, inflammation and autophagy-related proteins.Wheat peptides pretreatment significantly improved ethanol-induced weight loss in mice, increased antioxidant enzyme activity and nitric oxide (NO) content, and reduced lipid peroxidation in gastric tissues and GES-1 cells.Pathological tissue sections showed a significant reduction in gastric tissue damage after wheat peptides pretreatment.In addition, wheat peptides reduced tissue expression of pro-inflammatory factors and increased LC3-Ⅱ protein expression by inhibiting PI3K/AKT/mTOR pathway activation in GES-1 cells.Wheat peptides could improve ethanol-induced gastric mucosal injury by modulating antioxidant capacity, inhibiting pro-inflammatory factor expression and PI3K/mTOR pathway activation.
FENG Zhiyuan
,
ZHANG Zhuoran
,
REN Jie
,
WU Hanshuo
,
QIN Huimin
,
LIU Wenying
. Wheat peptides ameliorate ethanol-induced gastric mucosal damage by inhibiting inflammation and PI3K/mTOR pathway related proteins[J]. Food and Fermentation Industries, 2024
, 50(6)
: 92
-99
.
DOI: 10.13995/j.cnki.11-1802/ts.035328
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