Using the waste bamboo sawdust produced in the material preparation section of bamboo pulping plant as raw materials, bamboo-based cellulose after steam explosion pretreatment and extraction of hemicelluloses was used as carbon source.The bamboo sawdust before and after pretreatment were characterized by scanning electron microscopy & energy dispersive spectrometer and laser particle size analyzer.The key parameters of the fermentation process of L-lactic acid were analyzed by step hydrolysis fermentation and high performance liquid chromatography, and the potential of bioconversion of L-lactic acid from the carbon source was investigated.Results showed that steam explosion pretreatment increased the release of reducing sugar from the raw material, and the total sugar concentration reached 31.19 g/L under the conditions of 140 g/L of total solid concentration, 60 FPU/g of enzyme addition, and enzymatic hydrolysis for 54 h. The saccharification solution was used in the fermentation test.Under the conditions of initial sugar concentration of 19.43 g/L, initial pH 6.5, inoculation amount of 5%, and fermentation for 17 h, the L-lactic acid concentration could reach 6.28 g/L, and the saccharic acid conversion rate was as high as 80.87%.In conclusion,the experiment provides a reference for the utilization of bamboo-based cellulose.
YANG Chunliu
,
GUAN Xiuqiong
,
LIU Chun
,
HE Mingxiong
,
LIU Linpei
. Study on potential of bioconversion of L-lactic acid using bamboo-based cellulose as carbon source[J]. Food and Fermentation Industries, 2024
, 50(6)
: 177
-183
.
DOI: 10.13995/j.cnki.11-1802/ts.035121
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