Food and Fermentation Industries >

2024 , Vol. 50 >Issue 7: 51 - 60

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034355

Antioxidant activity of Schisandra sphenanthera and Schisandra chinensis protein on HepG2 cells

  • ZHOU Hongyan ,
  • ZHENG Yi ,
  • WANG Haidong ,
  • CAO Jun ,
  • ZHANG Han ,
  • ZHANG Hongyin ,
  • LI Guangzhe ,
  • YAN Mingming
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  • 1(Pharmaceutical Institute, Changchun University of Chinese Medicine, Changchun 130117, China)
    2(Northeast Asia Institute of Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China)
    3(Jilin Province Technology Innovation Center of Traditional Chinese Medicine Health Food, Changchun 130117, China)

Received date: 2022-11-18

  Revised date: 2023-01-01

  Online published: 2024-05-09

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Abstract

To study the free radical scavenging ability, reducing ability and repair effect on H2O2-induced oxidative damage in HepG2 cells of Schisandra sphenanthera protein(SSP) and Schisandra chinensis protein(SCP) in vitro, SSP and SCP were prepared by alkali extraction and acid precipitation method.The cell proliferation rate was detected by the CCK-8 method, and the intracellular reactive oxygen species level was detected by fluorescence.The activities of oxidative stress products and antioxidant enzymes were detected by enzyme-linked immunosorbent assay.Results showed that SSP was superior to SCP in scavenging free radicals and reducing power.Both SSP and SCP could repair the cell growth inhibition induced by H2O2 in HepG2 cells (P<0.05), significantly reduce the levels of intracellular reactive oxygen species and malondialdehyde (P<0.05), and significantly increase the levels of superoxide dismutase, catalase, glutathione peroxidase and glutathione (P<0.05).SSP was superior to SCP in repairing oxidative stress injury induced by H2O2 in HepG2 cells.In summary, SSP is more suitable for application in antioxidant functional food or the health food industry.

Key words： protein; free radical scavenging; oxidative stress; HepG2 cells; antioxidation

Cite this article

ZHOU Hongyan , ZHENG Yi , WANG Haidong , CAO Jun , ZHANG Han , ZHANG Hongyin , LI Guangzhe , YAN Mingming . Antioxidant activity of Schisandra sphenanthera and Schisandra chinensis protein on HepG2 cells[J]. Food and Fermentation Industries, 2024 , 50(7) : 51 -60 . DOI: 10.13995/j.cnki.11-1802/ts.034355

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