Food and Fermentation Industries >

2024 , Vol. 50 >Issue 7: 61 - 68

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038276

Physicochemical properties, antioxidant, and hypolipidemic effects of pectin from Malus pumila

  • LU Yongqiang ,
  • ZHOU Zhaoxi ,
  • ZHANG Lili ,
  • YU Yongting ,
  • NADIREMU·Xiaokelati ,
  • WANG Xinling ,
  • CONG Yuanyuan
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  • (College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China)

Received date: 2023-12-15

  Revised date: 2024-01-16

  Online published: 2024-05-09

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Abstract

This study aimed to investigate the physicochemical properties, antioxidant, and hypolipidemic effects of pectin from Malus pumila (MP).The molecular weight distribution of pectin was determined by high-performance gel permeation chromatography, and its physical and chemical properties, such as galacturonic acid content, esterification degree, and monosaccharide composition were determined.The possible structures were predicted by infrared (IR) spectroscopy and the antioxidant capacity of pectin was evaluated by antioxidant test in vitro.The rats were fed a high-fat diet (HFD) to induce hyperlipidemia and gavaged with MP.Body mass, serum lipid content, serum antioxidant index, and liver pathological changes of rats in each group were measured.Results showed that the molecular weight of MP was mainly distributed in 2-45 kDa, the content of galacturonic acid was 66.51% and the degree of esterification was 13.43%.The molar ratio of rhamnose, galacturonic acid, glucose, galactose, and arabinose was 0.60∶9.35∶0.58∶1.14∶0.96.Infrared spectrum analysis showed that MP had typical characteristic absorption peaks of polysaccharides, contained a pyranose ring, and had certain antioxidant capacity.In vivo hypolipidemic experiments showed that MP could alleviate mass gain in HFD-fed rats (P<0.05), reduce the increase of serum total triglycerides, total cholesterol, and low density lipoprotein cholesterol levels caused by HFD (P<0.05), increase the level of high density lipoprotein cholesterol (P<0.05), and improve the antioxidant status (P<0.05).MP also decreased the content of malondialdehyde (P<0.05) and reduced liver injury caused by HFD.Therefore, MP is an acidic polysaccharide with a low esterification degree, which has a strong antioxidant capacity and a significant hypolipidemic effect.This study provides data support for the systematic study of the physical and chemical properties and biological activity of commercial MP, and to provide ideas for MP to develop natural functional products.

Key words： Malus pumila; pectin; physicochemical properties; antioxidant; hypolipidemic

Cite this article

LU Yongqiang , ZHOU Zhaoxi , ZHANG Lili , YU Yongting , NADIREMU·Xiaokelati , WANG Xinling , CONG Yuanyuan . Physicochemical properties, antioxidant, and hypolipidemic effects of pectin from Malus pumila[J]. Food and Fermentation Industries, 2024 , 50(7) : 61 -68 . DOI: 10.13995/j.cnki.11-1802/ts.038276

References

[1] MASUELLI.Pectins-Extraction, Purification, Characterization and Applications[M].IntechOpen, 2020.
[2] JEDRYCHOWSKI W, MAUGERI U, PAC A, et al.Reduced risk of colorectal cancer and regular consumption of apples:Hospital based case-control study in Poland[J].Central European Journal of Medicine, 2009, 4(3):320-326.
[3] 李雪璨, 刘佳.苹果渣果胶超声波辅助提取工艺优化与抗氧化性研究[J].食品安全质量检测学报, 2022, 13(3):767-773.
LI X C, LIU J.Study on the ultrasonic-assisted extraction process optimization and antioxidant activity of pectin from apple pomace[J].Journal of Food Safety & Quality, 2022, 13(3):767-773.
[4] 孙立军, 郭玉蓉, 田兰兰.苹果果胶研究进展[J].食品工业科技, 2012, 33(4):445-449.
SUN L J,GUO Y R,TIAN L L.Research progress of apple pectin[J].Science and Technology of Food Industry, 2012, 33(4):445-449.
[5] GIRÓN-HERNÁNDEZ J, PAZMINO M, BARRIOS-RODRÍGUEZ Y F, et al.Exploring the effect of utilising organic acid solutions in ultrasound-assisted extraction of pectin from apple pomace, and its potential for biomedical purposes[J].Heliyon, 2023, 9(7):e17736.
[6] 张攀, 陈信, 熊双丽, 等.不同预处理对豆腐柴叶果胶理化性质的影响[J].食品工业科技, 2019, 40(1):82-86;91.
ZHANG P, CHEN X, XIONG S L, et al.Effects of different pretreatments on physicochemical properties of pectin from Premna microphylla Turcz leaves[J].Science and Technology of Food Industry, 2019, 40(1):82-86;91.
[7] 李楠, 张香飞, 杨春杰.板枣多糖初级结构表征及抗氧化活性[J].食品与机械, 2022, 38(10):24-28;49.
LI N, ZHANG X F, YANG C J.Primary structure characterization and antioxidant activity of polysaccharide from Zizyphus jujuba cv.Banzao[J].Food & Machinery, 2022, 38(10):24-28;49.
[8] 李衍衡, 赵明珠, 徐艺璇, 等.不同相对分子质量果胶多糖益生特性的比较研究[J].食品科技, 2022, 47(4):216-223.
LI Y H, ZHAO M Z, XU Y X, et al.Comparative study on probiotic properties of pectic polysaccharides with different relative molecular mass[J].Food Science and Technology, 2022, 47(4):216-223.
[9] 刘永玲, 赵建国, 赵治兵, 等.PMP柱前衍生hplc法测定八月瓜果皮多糖中单糖组成[J].食品研究与开发,2023,44(21):131-137.
LIU Y L, ZHAO J G, ZHAO Z B, et al.Determination of monosaccharide composition of polysaccharides from Akebia trifoliate Peel by PMP-HPLC with pre-column derivatization[J].Food Research and Development,2023,44(21):131-137.
[10] 李尧, 卢承蓉, 刘丹, 等.乳酸片球菌胞外多糖的分离纯化、结构分析及抗氧化活性研究[J].食品与发酵工业, 2021, 47(19):35-42.
LI Y, LU C R, LIU D, et al.Structure and antioxidant activity of Pediococcus lactis extracellular polysaccharide[J].Food and Fermentation Industries, 2021, 47(19):35-42.
[11] 丁子康, 李新月, 赵猛, 等.低分子量柘果多糖的制备及其抗氧化活性研究[J].食品工业科技, 2023, 44(19):39-46.
DING Z K, LI X Y, ZHAO M, et al.Study on the preparation and antioxidant activity of low molecular weight polysaccharide from Cudrania tricuspidata fruits[J].Science and Technology of Food Industry, 2023, 44(19):39-46.
[12] 刘欢, 赵巨堂, 何力, 等.金盏花精油的微波辅助提取及其成分与抗氧化活性研究[J].食品工业科技, 2022, 43(10):180-188.
LIU H, ZHAO J T, HE L, et al.Microwave-assisted extraction of essential oil from Calendula officinalis L.and its components and antioxidant activity[J].Science and Technology of Food Industry, 2022, 43(10):180-188.
[13] 张艳军, 李靖, 张玉领, 等.黄秋葵多糖对糖尿病大鼠糖脂代谢和肾脏损伤的改善作用[J].现代食品科技, 2021, 37(3):46-52.
ZHANG Y J, LI J, ZHANG Y L, et al.Okra polysaccharides improve glucose and lipid metabolism and kidney damage in diabetic rats[J].Modern Food Science and Technology, 2021, 37(3):46-52.
[14] ZHAO Y Y, BI J F, YI J Y, et al.Pectin and homogalacturonan with small molecular mass modulate microbial community and generate high SCFAs via in vitro gut fermentation[J].Carbohydrate Polymers, 2021, 269:118326.
[15] LUO S, WANG S D, YANG X D, et al.Gelation behaviors and mechanism of a new pectic polysaccharide from apple pomace as a potential gelatin substitute[J].International Journal of Biological Macromolecules, 2023, 241:124558.
[16] PAPPAS C S, MALOVIKOVA A, HROMADKOVA Z, et al.Determination of the degree of esterification of pectinates with decyl and benzyl ester groups by diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and curve-fitting deconvolution method[J].Carbohydrate Polymers, 2004, 56(4):465-469.
[17] GUO Z L, WU S Z, ZHENG H, et al.Effects of dielectric barrier discharge plasma modification on structure, physicochemical and functional properties of watermelon peel pectin[J].Food Hydrocolloids, 2024, 147:109350.
[18] 刘莎, 邓利玲, 钟耕, 等.酶解法制备臭黄荆叶果胶结构、理化性质、抗氧化和抗菌活性[J].食品科学,2023,44(23):55-63.
LIU S, DENG L L, ZHONG G, et al.Structure, physicochemical properties, antioxidant and antimicrobial activities of pectin from the Premna ligustroides Hemsl.leaves prepared by enzymatic processes[J].Food Science,2023,44(23):55-63.
[19] HU W W, CHENG H, WU D M, et al.Enhanced extraction assisted by pressure and ultrasound for targeting RG-I enriched pectin from citrus peel wastes:A mechanistic study[J].Food Hydrocolloids, 2022, 133:107778.
[20] ZHAO X L, MENG Y, LIU Y, et al.Pectic polysaccharides from Lilium brownii and Polygonatum odoratum exhibit significant antioxidant effects in vitro[J].International Journal of Biological Macromolecules, 2024,257:128830.
[21] 黄桂华. 高纯度海带岩藻聚糖的制备及其生物活性研究[D].泉州:华侨大学, 2018.
HUANG G H.Study on the preparation and bioactivity of high purity fucoidan from Laminaria japonica[D].Quanzhou:Huaqiao University, 2018.
[22] 王璐, 李蓓蓓, 王参政, 等.秋葵果胶对糖尿病小鼠降糖、降脂作用研究[J].中国食品添加剂, 2023, 34(9):236-243.
WANG L, LI B B, WANG C Z, et al.Effect of okra pectin on lowering blood sugar and lipid in diabetic mice[J].China Food Additives, 2023, 34(9):236-243.
[23] 唐茹萌, 焦文雅, 桑亚新, 等.裙带菜多糖体外和体内降血脂活性[J].食品科学, 2022, 43(1):142-149.
TANG R M, JIAO W Y, SANG Y X, et al.In vitro and in vivo hypolipidemic effect of Undaria pinnatifida polysaccharide[J].Food Science, 2022, 43(1):142-149.
[24] 刘雯, 车心怡, 马志超, 等.铜藻岩藻聚糖硫酸酯的结构组成及降血脂作用[J].大连海洋大学学报, 2023, 38(2):323-330.
LIU W, CHE X Y, MA Z C, et al.Structural composition and hypolipidemic effect of fucoidan from Sargassum horneri[J].Journal of Dalian Ocean University, 2023, 38(2):323-330.
[25] 王晶晶, 赵则月, 张晓霞, 等.黑莓果渣花青素降血糖、降血脂及抗氧化研究[J].中国食品添加剂, 2023, 34(8):214-219.
WANG J J, ZHAO Z Y, ZHANG X X, et al.Study on the hypoglycemic, hypolipidemic and antioxidant effects of anthocyanins from blackberry pomace[J].China Food Additives, 2023, 34(8):214-219.
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