Optimization of mixed strain fermentation process of Zha-chili by Fuzzy mathematics sensory evaluation combined with response surface methodology

  • HUANG Luhan ,
  • YIN Xiaoqing ,
  • KAN Jianquan ,
  • WU Yun ,
  • QI Chenchen ,
  • PENG Yun ,
  • SAMEH Awad ,
  • AMEL Ibrahim ,
  • DU Muying
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  • 1(College of Food Sciences, Southwest University, Chongqing 400715, China)
    2(Chinese-Hungarian Cooperative Research Centre for Food Science, Southwest University, Chongqing 400715, China)
    3(Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)
    4(Institute of Agricultural Products Development and Food Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lasa 850032, China)
    5(College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830000, China)
    6(Xinjiang Xinkang Agricultural Development Co.Ltd.,Urumqi 830000, China)
    7(Faculty of Agriculture, Alexandria University, Alexandria 999060, Egypt)

Received date: 2023-04-07

  Revised date: 2023-04-26

  Online published: 2024-05-09

Abstract

A mixed strain of fermented Zha-chili was prepared using fresh chili and corn flour as raw materials, Lactiplantibacillus plantarum XZ3 and aroma-producing yeast Y50 as strains.The fuzzy sensory score of the Zha-chili was used as the response value, and the Box-Behnken center combination design experiment was conducted to optimize the mixed strain fermentation process of the zha-chili and determine the functional composition of the product. The optimal fermentation conditions for the mixed strain of fermented Zha-chili were as follows:the ratio of L. plantarum XZ3 to aroma-producing yeast Y50 was 1∶1, the amount of mixed strain was 2.5%, the amount of salt was 4%, and the fermentation time was 6 days.Under these optimized conditions, the organoleptic score of the Zha-chili was 8.45.Compared with natural fermented Zha-chili without inoculation and pure fermented Zha-chili using L. plantarum XZ3, it is found that the aroma of the optimized mixed fermentation Zha-chili was more prominent.Further determined of the functional components revealed that the content of polyphenol components in mixed fermented Zha-chili was the highest and the most types were detected, with their total acid content, capsaicin content, and total flavonoid content significantly higher than those in naturally fermented Zha-chili.In addition, the content of sweet amino acids increased and the proportion of bitter amino acids decreased in the mixed strain of fermented Zha-chili compared to the other two fermentation methods.This study provides a useful reference for the production of mixed strains of fermented Zha-chili.

Cite this article

HUANG Luhan , YIN Xiaoqing , KAN Jianquan , WU Yun , QI Chenchen , PENG Yun , SAMEH Awad , AMEL Ibrahim , DU Muying . Optimization of mixed strain fermentation process of Zha-chili by Fuzzy mathematics sensory evaluation combined with response surface methodology[J]. Food and Fermentation Industries, 2024 , 50(7) : 83 -91 . DOI: 10.13995/j.cnki.11-1802/ts.035754

References

[1] CAI W C, TANG F X, WANG Y R, et al.Bacterial diversity and flavor profile of Zha-Chili, a traditional fermented food in China[J].Food Research International, 2021, 141:110112.
[2] DONG W W, SHEN H Y, LIU H M, et al.Unraveling the microbial community and succession during Zha-chili fermentation and their relationships with flavor formation[J].Food Research International, 2022, 157:111239.
[3] 汤艳燕. 鲊辣椒发酵中微生物群落结构及其对风味物质的影响[D].重庆:西南大学, 2019.
TANG Y Y.Microbial community structure and its influence on flavor substances in the fermentation of Chinese hot pepper[D].Chongqing:Southwest University, 2019.
[4] GUO Z, WANG Y R, XIANG F S, et al.Evaluating the flavor and divergent bacterial communities in corn-based Zha-chili[J].Food Bioscience, 2022, 46:101563.
[5] 莫玉花. 包谷酸辣子自然发酵过程中微生物变化、优势菌筛选及应用研究[D].长沙:湖南农业大学, 2017.
MO Y H.Microbial Changes, screening of Dominant Microbes and Its applicantion during natural fermentation of spicy Bao Gu Suan[D].Changsha:Hunan Agricultural University, 2017.
[6] 陈大鹏, 郑娅, 周芸, 等.自然发酵与人工接种发酵法发酵泡菜的品质比较[J].食品工业科技, 2019, 40(18):368-372.
CHEN D P, ZHENG Y, ZHOU Y, et al.Quality comparison of pickled cabbage fermented by natural fermentation and artificial inoculation[J].Science and Technology of Food Industry, 2019, 40(18):368-372.
[7] STEENSELS J, MEERSMAN E, SNOEK T, et al.Large-scale selection and breeding to generate industrial yeasts with superior aroma production[J].Applied and Environmental Microbiology, 2014, 80(22):6965-6975.
[8] 崔小利, 王薇, 阚建全.鲊辣椒纯种发酵的菌株优选[J].食品科学, 2014, 35(21):149-153.
CUI X L, WANG W, KAN J Q.Screening strains for use in pure culture fermentation for the production of zhalajiao, a traditional Chinese fermented hot pepper product[J].Food Science, 2014, 35(21):149-153.
[9] 姚红, 尹小庆, 颜宇鸽, 等.鲊辣椒生香酵母的分离筛选鉴定及其应用研究[J].食品与发酵工业, 2023, 49(17):145-152.
YAO H, YIN X Q, YAN Y G, et al.Application of an aroma-producing yeast isolated from natural fermented Zha-chili[J].Food and Fermentation Industries, 2023, 49(17):145-152.
[10] BASHIR MIR M, RASTOGI S, HARIPRIYA S.Optimization of process variables for the preparation of almond gum incorporated set-yogurt using Box-Behnken response surface model[J].Applied Food Research, 2021, 1(2):100016.
[11] 白娟, 张瑶, 汪雪瑞, 等.响应面法优化鲊辣椒的发酵工艺[J].中国酿造, 2018, 37(10):106-110.
BAI J, ZHANG Y, WANG X R, et al.Optimization of fermentation process of preserved pepper by response surface methodology[J].China Brewing, 2018, 37(10):106-110.
[12] 肖星凝, 李苇舟, 石芳, 等.不同品种李子多酚组成及抗氧化活性[J].食品科学, 2017, 38(15):31-37.
XIAO X N, LI W Z, SHI F, et al.Antioxidant activity and phenolic contents of peel and pulp of different plum varieties[J].Food Science, 2017, 38(15):31-37.
[13] 李苇舟, 李福香, 何晓琴, 等.酿造啤酒大麦发芽前后多酚组分变化及抗氧化活性[J].食品科学, 2019, 40(18):15-21.
LI W Z, LI F X, HE X Q, et al.Changes in phenloic components and antioxidant activity of barley before and after malting[J].Food Science, 2019, 40(18):15-21.
[14] HUANG Z T, CHEN Q Q, HU K X, et al.Effects of in vitro simulated digestion on the free and bound phenolic content and antioxidant activity of seven species of seaweeds[J].International Journal of Food Science & Technology, 2021, 56(5):2365-2374.
[15] LIU X M, LIU Y, SHAN C H, et al.Effects of five extraction methods on total content, composition, and stability of flavonoids in jujube[J].Food Chemistry:X, 2022, 14:100287.
[16] 尹小庆, 汤艳燕, 阚建全, 等.小米辣鲊辣椒发酵过程中风味物质及微生物多样性的变化[J].食品与发酵工业, 2020, 46(5):74-82.
YIN X Q, TANG Y Y, KAN J Q, et al.Analysis of flavor components and microbial diversity of Xiaomila Zhalajiao during fermentation[J].Food and Fermentation Industries, 2020, 46(5):74-82.
[17] 李庆羊, 吴祖芳, 翁佩芳, 等.植物乳杆菌和棒状乳杆菌对发酵萝卜干风味品质的影响[J].中国食品学报, 2021, 21(9):150-159.
LI Q Y, WU Z F, WENG P F, et al.Effects of Lactobacillus plantarum and Lactobacillus rodarum on flavor quality of fermented dried radish[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(9):150-159.
[18] JAFARI M, REZAEI M, GHEISARI H R, et al.Application of cultivable lactic acid bacteria isolated from Iranian traditional dairy products for the production of liquid and dried kashks[J].LWT, 2019, 116:108519.
[19] 侯爱香, 王一淇, 黄晴, 等.自然发酵与人工接种发酵湖南芥菜的挥发性风味组分和品质分析[J].食品科学, 2018, 39(6):237-245.
HOU A X, WANG Y Q, HUANG Q, et al.Comparison of volatile flavor compounds and qualities between naturally fermented and inoculated Chinese leaf mustard(Brassica juncea coss.) grown in Hunan Province, China[J].Food Science, 2018, 39(6):237-245.
[20] 阙小峰, 黄超群, 方志成, 等.天然辣椒碱提取方法及在食品中应用的研究进展[J].中国调味品, 2021, 46(9):188-192.
QUE X F, HUANG C Q, FANG Z C, et al.Research progress on extraction methods of natural capsaicin and its application in food[J].China Condiment, 2021, 46(9):188-192.
[21] WU Q Y, BAI P Z, XIA Y T, et al.Capsaicin inhibits the expression of melanogenic proteins in melanocyte via activation of TRPV1 channel:Identifying an inhibitor of skin melanogenesis[J].Journal of Agricultural and Food Chemistry, 2020, 68(50):14863-14873.
[22] 武亚婷. 基于Miseq高通量测序技术对低盐发酵辣椒酱品质提升的研究[D].乌鲁木齐:新疆农业大学, 2020.
WU Y T.Study on improving the quality of low-salt fermented Chili sauce based on Miseq high-throughput sequencing technology[D].Urumqi:Xinjiang Agricultural University, 2020.
[23] LI W, CHEN H H, XU B, et al.Research progress on classification, sources and functions of dietary polyphenols for prevention and treatment of chronic diseases[J].Journal of Future Foods, 2023, 3(4):289-305.
[24] 葛平珍, 王丹, 周才琼.不同淀粉源对鲊海椒发酵过程中功能成分的影响[J].食品科学, 2015, 36(21):191-195.
GE P Z, WANG D, ZHOU C Q.Functional components of Zhahaijiao, a traditional Chinese fermented chili product, mixed with starches from different botanical sources during fermentation[J].Food Science, 2015, 36(21):191-195.
[25] 代晓桐. 发酵型铁皮石斛辣椒酱的研制[D].贵阳:贵州大学, 2022.
DAI X T.Development of fermented Dendrobium officinale chili sauce[D].Guiyang:Guizhou University, 2022.
[26] 王晓梅, 奚宇, 范新光, 等.绿原酸的生物利用率和抗氧化活性研究进展[J].中国食品学报, 2019, 19(1):271-279.
WANG X M, XI Y, FAN X G, et al.Research progress on bioavailability and antioxidant activity of chlorogenic acid[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(1):271-279.
[27] 徐畅, 刘意隆, 高志伟, 等.杨梅素及其苷类药理活性研究进展[J].中国中药杂志, 2020, 45(15):3575-3583.
XU C, LIU Y L, GAO Z W, et al.Pharmacological activities of myricetin and its glycosides[J].China Journal of Chinese Materia Medica, 2020, 45(15):3575-3583.
[28] 祁翔. 亮氨酸补充对老龄大鼠骨骼肌生长的影响及机理探讨[D].上海:上海体育学院, 2015.
QI X.The research of the effects of leucine supplement in older people muscle protein metabolism[D].Shanghai:Shanghai University of Sport, 2015.
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