Food and Fermentation Industries >

2024 , Vol. 50 >Issue 8: 17 - 23

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035243

Protective effect of bergamot polysaccharide on SH-SY5Y cells injured by 1-methyl-4-phenyl-pyridine ion

  • CHEN Jinxuan ,
  • GONG Shu ,
  • LIU Tiankai ,
  • GONG Jiyi ,
  • YI Yin ,
  • LIU Wenhua
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  • 1(School of Life Science, Guizhou Normal University, Guiyang 550025, China)
    2(School of Life Science, Zhaoqing University, Zhaoqing 526061, China)

Received date: 2023-02-24

  Revised date: 2023-03-27

  Online published: 2024-06-11

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Abstract

This study aimed to explore the protective effect and mechanism of bergamot polysaccharide on damaged SH-SY5Y cells induced by 1-methyl-4-phenyl-pyridine ion (MPP+).The bergamot polysaccharide was extracted and purified with AB-8 macroporous resin.SH-SY5Y cells were cultured in vitro to construct a Parkinson's disease (PD) cell model.The experiment was divided into a control group, the MPP+ model group, and the bergamot polysaccharide group.The cell viability was detected with the methye thiazdye telrazlium (MTT) method, and the cell morphology was observed by the Hoechst33258 staining method.The reactive oxygen species (ROS) level was detected by the DCFH-DA fluorescence probe, and mitochondrial membrane potential was measured by the JC-1 fluorescence probe method.The protein expression levels of phosphorylated protein kinase B (p-Akt), protein kinase B (Akt), phosphorylated extracellular regulated protein kinases1/2 (p-ERK1/2), and cytochrome c (Cyt-c) were detected by Western blot.Results showed that the yield of bergamot polysaccharide was 4.86% and the purity was 44.46%.After purification by AB-8, the purity of the polysaccharide was increased to 60.81%.Compared with the control group, the cell viability of the MPP+-treated group was significantly reduced, the cells were broken and the nucleus was shrunk after Hoechst33258 staining.Meanwhile, the intracellular ROS was significantly increased, and the mitochondrial membrane potential was significantly decreased.Compared with the model group, the cell viability of the bergamot polysaccharide group was significantly increased, the cell morphology was significantly improved, the enhancement of intracellular ROS was inhibited, and the mitochondrial membrane potential was increased.The results of the Western blot showed that bergamot polysaccharide could inhibit the decrease of p-Akt and p-ERK1/2 as well as the increase of Cyt-c caused by MPP+.Based on these data, bergamot polysaccharide had a protective effect on SH-SY5Y cells against the injury induced by MPP+, which might be correlated with antioxidant activity as well as ERK and Akt activation pathway.The research results could provide a theoretical basis for alleviating the occurrence and development of Parkinson's disease, and be also beneficial for the development and utilization of bergamot resources.

Key words： bergamot polysaccharide; extraction and purification; 1-methyl-4-phenyl-pyridine ion (MPP+); SH-SY5Y cells; protective effect

Cite this article

CHEN Jinxuan , GONG Shu , LIU Tiankai , GONG Jiyi , YI Yin , LIU Wenhua . Protective effect of bergamot polysaccharide on SH-SY5Y cells injured by 1-methyl-4-phenyl-pyridine ion[J]. Food and Fermentation Industries, 2024 , 50(8) : 17 -23 . DOI: 10.13995/j.cnki.11-1802/ts.035243

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