Food and Fermentation Industries >

2024 , Vol. 50 >Issue 8: 24 - 30

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036300

Diversity and protease hydrolysis characteristics of psychrophilic in raw milk and pasture environment

  • HU Shaozhen ,
  • DAI Liangchao ,
  • ZHANG Shuwen ,
  • LYU Jiaping ,
  • LI Lianrui ,
  • PANG Xiaoyang
Expand
  • 1(College of Life Sciences and Technology, College of Animal Sciences and Technology, Tarim University, Alaer 843300, China)
    2(Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
    3(College of Food Science, Shenyang Agricultural University, Shenyang 110866, China)

Received date: 2023-05-30

  Revised date: 2023-06-22

  Online published: 2024-06-11

Fold

Abstract

To explore the diversity and enzyme production of psychrophilic in raw milk and pasture environment, raw milk and habitat samples from different links were collected from large-scale pastures in four regions of China.Based on axenic culture technology, the isolation and identification of psychrophilic were carried out, and the diversity of psychrophilic was analyzed, so as to clarify the pollution distribution of psychrophilic.The protease activity of Pseudomonas and alkaline peptidase gene (aprX) were further detected.Results showed that a total of 329 strains of psychrophilic were isolated from 64 samples, which were classified into 21 genera and 76 species.Pseudomonas was the main polluting strain in the pasture, accounting for 57.45% of the total isolates.At 28 ℃, 61.38% of Pseudomonas strains could produce protease, and up to 92.24% of Pseudomonas strains carried aprX gene.After being treated at 135 ℃ for 5 s, the protease residue rate of 21.55% was more than 60%.In this study, there were many kinds of psychrophilic in raw milk and pasture environment, and the ability of heat-resistant protease production was strong.

Key words： Pseudomonas; pollution distribution; protease activity; aprX

Cite this article

HU Shaozhen , DAI Liangchao , ZHANG Shuwen , LYU Jiaping , LI Lianrui , PANG Xiaoyang . Diversity and protease hydrolysis characteristics of psychrophilic in raw milk and pasture environment[J]. Food and Fermentation Industries, 2024 , 50(8) : 24 -30 . DOI: 10.13995/j.cnki.11-1802/ts.036300

References

[1] VITHANAGE N R, DISSANAYAKE M, BOLGE G, et al.Biodiversity of culturable psychrotrophic microbiota in raw milk attributable to refrigeration conditions, seasonality and their spoilage potential[J].International Dairy Journal, 2016, 57:80-90.
[2] PAULDAS K, EASOW J, NAIR S, et al.Microbial quality control of raw milk and isolation of spoilage causing microorganisms[J].Advances in Microbiology, 2022, 3:56-64.
[3] JIN S S, WANG Y Z, ZHAO X H.Cold-adaptive mechanism of psychrophilic bacteria in food and its application[J].Microbial Pathogenesis, 2022,169:105652.
[4] ZHANG S, LI H, ULUKO H, et al.Investigation of protease production by Pseudomonas fluorescens BJ-10 and degradation on milk proteins[J].Journal of Food Processing and Preservation, 2015, 39(6):2466-2472.
[5] ZHANG C Y, BIJL E, SVENSSON B, et al.The extracellular protease AprX from Pseudomonas and its spoilage potential for UHT milk:A review[J].Comprehensive Reviews in Food Science and Food Safety, 2019, 18(4):834-852.
[6] FOROUTAN A, GUO A C, VAZQUEZ-FRESNO R, et al.Chemical composition of commercial cow's milk[J].Journal of Agricultural and Food Chemistry, 2019, 67(17):4897-4914.
[7] TEIDER P I, RIBEIRO J C, OSSUGUI E H, et al.Pseudomonas spp.and other psychrotrophic microorganisms in inspected and non-inspected Brazilian Minas Frescal cheese:Proteolytic, lipolytic and AprX production potential[J].Pesquisa Veterinária Brasileira, 2019, 39(10):807-815.
[8] DU B Y, MENG L, LIU H M, et al.Single molecule real-time sequencing and traditional cultivation techniques reveal complex community structures and regional variations of psychrotrophic bacteria in raw milk[J].Frontiers in Microbiology, 2022, 13:853263.
[9] LIANG L J, WANG P, ZHAO X M, et al.Single-molecule real-time sequencing reveals differences in bacterial diversity in raw milk in different regions and seasons in China[J].Journal of Dairy Science, 2022, 105(7):5669-5684.
[10] KUMAR S, TAMURA K, NEI M.MEGA:Molecular evolutionary genetics analysis software for microcomputers[J].Bioinformatics, 1994, 10(2):189-191.
[11] SCATAMBURLO T M, YAMAZI A K, CAVICCHIOLI V Q, et al.Spoilage potential of Pseudomonas species isolated from goat milk[J].Journal of Dairy Science, 2015, 98(2):759-764.
[12] MENG L, LIU H M, DONG L, et al.Identification and proteolytic activity quantification of Pseudomonas spp.isolated from different raw milks at storage temperatures[J].Journal of Dairy Science, 2018, 101(4):2897-2905.
[13] CALDERA L, FRANZETTI L, VAN COILLIE E, et al.Identification, enzymatic spoilage characterization and proteolytic activity quantification of Pseudomonas spp.isolated from different foods[J].Food Microbiology, 2016, 54:142-153.
[14] TAMURA K, DUDLEY J, NEI M, et al.MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0[J].Molecular Biology and Evolution, 2007, 24(8):1596-1599.
[15] BECKER R.The New S Language [M].CRC Press, 2018.
[16] DECIMO M, MORANDI S, SILVETTI T, et al.Characterization of gram-negative psychrotrophic bacteria isolated from italian bulk tank milk[J].Journal of Food Science, 2014, 79(10):M2081-M2090.
[17] HAHNE J, ISELE D, BERNING J, et al.The contribution of fast growing, psychrotrophic microorganisms on biodiversity of refrigerated raw cow's milk with high bacterial counts and their food spoilage potential[J].Food Microbiology, 2019, 79(JUN.):11-19.
[18] LONGHI R, CORREIA S S, BRUZAROSKI S R, et al.Pseudomonas fluorescens and Pseudomonas putida from refrigerated raw milk:Genetic diversity and lipoproteolytic activity[J].The Journal of Dairy Research, 2022, (89-1):86-89.
[19] MARCHAND S,HEYLEN K,MESSENS W, et al.Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples[J].Environmental Microbiology, 2009, 11(2):467-482.
[20] DU B Y, MENG L, LIU H M, et al.Diversity and proteolytic activity of Pseudomonas species isolated from raw cow milk samples across China[J].The Science of the Total Environment, 2022, 838(Pt 3):156382-156389.
[21] NARVHUS J A, NILSEN B A, EKKELUND O, TIDEMANN E M, et al.Isolates of Pseudomonas spp.from cold-stored raw milk show variation in proteolytic and lipolytic properties[J].International Dairy Journal, 2021, 123:105049.
[22] YANG X Y, GUO X J, LIU W P, et al.The complex community structures and seasonal variations of psychrotrophic bacteria in raw milk in Heilongjiang Province, China[J].LWT- Food Science and Technology, 2020, 134:110218-110229.
[23] 刘潇忆, 张彧, 陈历俊, 等.原料乳生产过程中微生物污染的来源追溯研究[J].中国乳品工业, 2013, 41(6):51-55;64.
LIU X Y, ZHANG Y, CHEN L J, et al.Study on the microbial contaminations source traceability of raw milk processing.[J].China Dairy Industry, 2013, 41(6):51-55;64.
[24] BANYKÓ J, VYLETELOVÁ M.Determining the source of Bacillus cereus and Bacillus licheniformis isolated from raw milk, pasteurized milk and yoghurt[J].Letters in Applied Microbiology, 2009, 48(3):318-323.
[25] DOYLEAB C J, GLEESONC D, O'TOOLEBD P W, et al.High-throughput sequencing highlights the significant influence of seasonal housing and teat preparation on the raw milk microbiota[J].Applied and Environmental Microbiology, 2016:AEM.02694-02709.
[26] ZAREI M, YOUSEFVAND A, MAKTABI S, et al.Identification, phylogenetic characterisation and proteolytic activity quantification of high biofilm-forming Pseudomonas fluorescens group bacterial strains isolated from cold raw milk[J].International Dairy Journal, 2020, 109:104787-104795.
[27] COLANTUONO A, D'INCECCO P, FORTINA M G, et al.Milk substrates influence proteolytic activity of Pseudomonas fluorescens strains[J].Food Control, 2020, 111:107063-107071.
[28] ZHANG D, LI S Q, PALMER J, et al.The relationship between numbers of Pseudomonas bacteria in milk used to manufacture UHT milk and the effect on product quality[J].International Dairy Journal, 2020, 105:104687.
[29] ZHANG D, PALMER J, TEH K H, et al.Milk fat influences proteolytic enzyme activity of dairy Pseudomonas species[J].International Journal of Food Microbiology, 2020, 320:108543.
[30] MENG L, ZHANG Y D, LIU H M, et al.Characterization of Pseudomonas spp.and associated proteolytic properties in raw milk stored at low temperatures[J].Frontiers in Microbiology, 2017, 8:2158.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.