Food and Fermentation Industries >

2024 , Vol. 50 >Issue 8: 97 - 104

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035774

Optimization of culture conditions and extraction process of polysaccharide from Sparassis latifolia by liquid fermentation

  • LIU Pengxiao ,
  • GAO Su ,
  • LIANG Ruina ,
  • AN Miaomiao ,
  • ZHAO Guozhu
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  • (College of Biological Sciences and Technology, Beijing Forestry University, Beijing Key Laboratory of Food Processing and Safety in Forestry, Beijing 100083, China)

Received date: 2023-04-10

  Revised date: 2023-06-05

  Online published: 2024-06-11

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Abstract

Sparassis latifolia is valuable edible and medicinal mushroom.Polysaccharide is an important active ingredient of S.latifolia, and low molecular weight polysaccharides have prominent antioxidant and antitumor effects.To improve the production of liquid fermented mycelium of S.latifolia and intracellular low molecular weight polysaccharide and increase the extraction efficiency, the single factor method and response surface method were used to optimize the culture conditions and extraction process.The results of single factor optimization verification showed that the carbon source of liquid fermentation medium was soluble starch (15 g/L), the nitrogen source was beef extract (8 g/L), the pH was 5.After 17 days of fermentation, the mycelium dry weight and intracellular polysaccharide of S.latifolia XQJ-1 were (6.03±0.25) g/L and (433.25±37.46) mg/L, respectively, which was at a high optimization level.The optimization of XQJ-1 intracellular polysaccharide extraction by response surface method showed that the intracellular polysaccharide yield was (8.82±0.15)% when the extraction times were twice, the extraction time was 2.4 h, the liquid-solid ratio was 31∶1(mL∶g), and the temperature was 89 ℃, indicating a highly efficient extraction process.The results provide a reference for the further development and utilization of S.latifolia and its low molecular weight polysaccharide.

Key words： Sparassis latifolia; intracellular polysaccharide; culture days; medium conditions; extraction process; response surface methodology

Cite this article

LIU Pengxiao , GAO Su , LIANG Ruina , AN Miaomiao , ZHAO Guozhu . Optimization of culture conditions and extraction process of polysaccharide from Sparassis latifolia by liquid fermentation[J]. Food and Fermentation Industries, 2024 , 50(8) : 97 -104 . DOI: 10.13995/j.cnki.11-1802/ts.035774

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