Food and Fermentation Industries >

2024 , Vol. 50 >Issue 8: 105 - 113

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035027

Protective effects of mulberry anthocyanin on oxidative stress in PC12 cells

  • ZHU Xiaohan ,
  • TIE Fangfang ,
  • OUYANG Jian ,
  • WANG Honglun ,
  • YIN Jungang
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  • 1(College of Life Sciences, Yantai University, Yantai 264005, China)
    2(Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China)
    3(Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining 810008, China)

Received date: 2023-02-09

  Revised date: 2023-04-04

  Online published: 2024-06-11

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Abstract

The protective function and related mechanism against H2O2-induced cell injury of cyanidin compounds cyanidin-3-O-glucoside (C3G) and cyanidin-3-O-rutinoside (C3R) was investigated by establishing the model of an oxidative stress injury in PC12 cells.MTT assay and DCFH-DA fluorescent probe were employed to detect cell viability and reactive oxygen species (ROS) content, respectively.The test kits were applied to detect reduced glutathione (GSH), malondialdehyde (MDA) content, superoxide dismutase (SOD), and catalase (CAT) activity.Antioxidant and apoptotic protein expression level was detected by Western blot.Results showed that the cell survival rate was significantly decreased treated with 650 μmol/L H2O2 for 12 hours (P<0.01), 50 μmol/L and 100 μmol/L C3G and C3R could improve the survival rate of PC12 cells, inhibit the production of ROS, decrease the content of MDA, and enhance the activities of GSH, SOD, and CAT in PC12 cells compared with H2O2 treatment group (P<0.05).Western blot results showed that 50 μmol/L and 100 μmol/L C3G and C3R could significantly inhibit the phosphorylation levels of c-Jun N-terminal kinase (JNK) and extracellular regulated protein kinases (ERK1/2) proteins as well as the expression of Caspase-3 and Bcl-2-associated X protein (Bax), and promote the protein levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) (P<0.05).In summary, C3G and C3R have protective effects on H2O2-induced oxidative damage in PC12 cells which may be related to inhibiting the expression of apoptosis-related proteins, improving the activity of intracellular antioxidant enzymes, clearing excess ROS in cells, and reducing the rate of apoptosis.

Key words： mulberry anthocyanin; cyanidin-3-O-glucoside; cyanidin-3-O-rutinoside; PC12 cells; oxidative stress; apoptosis

Cite this article

ZHU Xiaohan , TIE Fangfang , OUYANG Jian , WANG Honglun , YIN Jungang . Protective effects of mulberry anthocyanin on oxidative stress in PC12 cells[J]. Food and Fermentation Industries, 2024 , 50(8) : 105 -113 . DOI: 10.13995/j.cnki.11-1802/ts.035027

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