Natural polysaccharides have rich active functions.In order to further explore the pathway of activity and to determine the probiotic potential of Armillariella tabescens polysaccharides, this study prepared Armillariella tabescens extracellular polysaccharide (ATEP) by liquid fermentation, and evaluated the physicochemical compositions, the antioxidant capacity and the changes rule during different stages of the simulated digestion-fermentation process.The results showed that the total sugar content of ATEP was 43.52%, the molecular weights of the two components of ATEP were 27.0 kDa and 8.9 kDa, respectively.ATEP was composed of six monosaccharides.The simulated digestion process hardly promoted the total sugar and reducing sugar contents (P>0.05), but could increase the total phenol and total flavonoid contents (P<0.05).During the simulated fermentation process, the relative consumption of carbohydrates gradually rose, reducing sugar falling down after growing, while pH continued to decrease.The contents of SCFAs in fermental production increased significantly (P<0.05), and the acetic acid was the highest.ATEP promoted the proliferation rate of four probiotics, Bifidobacterium, Lactobacillus plantarum, Lactobacillus casei and Escherichia coli.This study evaluates the probiotic level of ATEP, and provides ideas and basic theoretical basis for the comprehensive evaluation and utilization of it.
[1] 景永帅, 孙丽丛, 张瑞娟, 等.玉竹多糖的体外消化特性及其与乳杆菌和大肠杆菌的相互作用[J].食品研究与开发, 2023, 44(2):21-28.
JING Y S, SUN L C, ZHANG R J, et al.In vitro digestion characteristics of Polygonatum odoratum polysaccharides and their interaction with Lactobacillus bulgaricus and Escherichia coli[J].Food Research and Development, 2023, 44(2):21-28.
[2] LAGAMMA E F, HU F R, PENA CRUZ F, et al.Bacteria - derived short chain fatty acids restore sympathoadrenal responsiveness to hypoglycemia after antibiotic-induced gut microbiota depletion[J].Neurobiology of Stress, 2021, 15:100376.
[3] KIHO T, SHIOSE Y, NAGAI K, et al.Polysaccharides in fungi.XXIX.Structural features of two antitumor polysaccharides from the fruiting bodies of Armillariella tabescens[J].Chemical & Pharmaceutical Bulletin, 1992, 40(8):2212-2214.
[4] YANG R, LI Y D, MEHMOOD S, et al.Polysaccharides from Armillariella tabescens mycelia ameliorate renal damage in type 2 diabetic mice[J].International Journal of Biological Macromolecules, 2020, 162:1682-1691.
[5] YANG R, LI Y D, CAI J J, et al.Polysaccharides from Armillariella tabescens mycelia ameliorate insulin resistance in type 2 diabetic mice[J].Food & Function, 2020, 11(11):9675-9685.
[6] HU Z T, ONDREJCAK T, YU P P, et al.Do tau-synaptic long-term depression interactions in the hippocampus play a pivotal role in the progression of Alzheimer’s disease?[J].Neural Regeneration Research, 2023, 18(6):1213-1219.
[7] HENRIQUEZ C, ALIAGA C, LISSI E.Kinetics profiles in the reaction of abts derived radicals with simple phenols and polyphenols[J].Journal of the Chilean Chemical Society, 2004, 49(1):65-67.
[8] 陈君诚. 知母多糖的结构鉴定、降血糖机制及其对肠道菌群的影响[D].广州:华南理工大学, 2021.
CHEN J C.Structural identification and hypoglycemic activity of polysaccharides from anemarrhena asphodeloides bunge and their effect on gut microflora[D].Guangzhou:South China University of Technology, 2021.
[9] KASIRGA B, DEMIR E A, DOGAN H, et al.Determining the ideal object size in the novel object recognition test[J].ACTA Physiologica, 2019, 227:96.
[10] SAEED A, MARWAT M S, SHAH A H, et al.Assessment of total phenolic and flavonoid contents of selected fruits and vegetables[J].Indian Journal of Traditional Knowledge, 2019, 18(4):686-693.
[11] ZAMEER S, HUSSAIN S, VOHORA D, et al.Alendronate reduces the cognitive and neurological disturbances induced by combined doses of D-galactose and aluminum chloride in mice[J].Journal of Applied Toxicology:JAT, 2021, 41(11):1779-1793.
[12] COLMAN S L, MASSA G A, CARBONI M F, et al.Cold sweetening diversity in Andean potato germplasm from Argentina[J].Journal of the Science of Food and Agriculture, 2017, 97(14):4744-4749.
[13] CHEN J, WU Y, YANG C M, et al.Antioxidant and hypolipidemic effects of soymilk fermented via Lactococcus acidophilus MF204[J].Food & Function, 2017, 8(12):4414-4420.
[14] 曲航. 松仁多糖结构表征及对化学性肝损伤防护作用机制研究[D].哈尔滨:哈尔滨工业大学, 2020.
QU H.Structural Characterization of Pinus koraiensis pine nut polysaccharide and its hepatoprotection mechanisms against chemical induced liver injury[D].Harbin:Harbin Institute of Technology, 2020.
[15] ZHANG W Y, HU B, LIU C, et al.Comprehensive analysis of Sparassis crispa polysaccharide characteristics during the in vitro digestion and fermentation model[J].Food Research International, 2022, 154:111005.
[16] 曹长靓. 海蒿子多糖的分离纯化、降血糖活性及其体外消化酵解研究[D].广州:华南理工大学, 2021.
CAO C L.Isolation, Purification, hypoglycemic activity and in vitro digestion and fermentation of polysaccharides from Sargassum pallidum[D].Guangzhou:South China University of Technology, 2021.
[17] 高洁. 海带多糖的结构表征及其对血脂异常相关肠道菌群的影响研究[D].广州:华南理工大学, 2019.
GAO J.Study on Structural characterization of Laminaria japonica polysaccharide and its beneficial effects on dyslipidemias-associated intestinal bacteria[D].Guangzhou:South China University of Technology, 2019.
[18] 廖玉琴, 韩耀辉, 任中阳, 等.微波处理和干制时间对干鲍体外模拟消化产物抗氧化活性的影响[J].食品科学, 2023, 44(23):114-122.
LIAO Y Q, HAN Y H, REN Z Y, et al.Effect of microwave treatment and drying time on the antioxidant activity of dried abalone hydrolysate prepared by simulated digestionin vitro[J].Food Science, 2023, 44(23):114-122.
[19] SUDHEER S M, RABA G, KAPITANOV I V, et al.A greener approach to hydrolyse ionic liquids[J].Basic & Clinical Pharmacology & Toxicology, 2018.DOI:10.1111/bcpt.13165.
[20] 徐倩, 王晗, 马赛, 等.杏鲍菇多糖及其消化产物对淀粉消化酶的抑制及相互作用[J].中国农业科学, 2023, 56(2):357-367.
XU Q, WANG H, MA S, et al.Inhibition and interaction of Pleurotus eryngii polysaccharide and its digestion products on starch digestive enzymes[J].Scientia Agricultura Sinica, 2023, 56(2):357-367.
[21] 梁美香, 杨可, 李康杰, 等.羧甲基化柑橘果胶的体外模拟消化及益生元作用[J/OL].食品与发酵工业, 2023.DOI:10.13995/j.cnki.11-1802/ts.035229.
LIANG M X, YANG K, LI K J, et al.In vitro simulated digestion and intestinal prebiotic effect of carboxymethyl citrus pectin[J/OL].Food and Fermentation Industry, 2023.DOI:10.13995/j.cnki.11-1802/ts.035229.
[22] YUAN D, LI C, YOU L J, et al.Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota[J].International Journal of Biological Macromolecules, 2020, 164:1443-1450.
[23] PIMENTEL F B, CERMEÑO M, KLEEKAYAI T, et al.Effect of in vitro simulated gastrointestinal digestion on the antioxidant activity of the red seaweed Porphyra dioica[J].Food Research International, 2020, 136:109309.
[24] ZHU K X, YAO S W, ZHANG Y J, et al.Effects of in vitro saliva, gastric and intestinal digestion on the chemical properties, antioxidant activity of polysaccharide from Artocarpus heterophyllus Lam.(jackfruit) Pulp[J].Food Hydrocolloids, 2019, 87:952-959.
[25] HAN R, PANG D R, WEN L R, et al.In vitro digestibility and prebiotic activities of a sulfated polysaccharide from Gracilaria lemaneiformis[J].Journal of Functional Foods, 2020, 64:103652.
[26] XIE Z Q, WANG S K, WANG Z G, et al.In vitro fecal fermentation of propionylated high-amylose maize starch and its impact on gut microbiota[J].Carbohydrate Polymers, 2019, 223:115069.
