Food and Fermentation Industries >

2024 , Vol. 50 >Issue 9: 139 - 147

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035511

Structural characteristics and immunomodulatory activity of raspberry polysaccharide RPP-5

  • YIN Xingxing ,
  • YANG Yongjing ,
  • LU Jie ,
  • WANG Xuehong ,
  • CHAO Mu
Expand
  • 1(School of Ecological and Environmental Engineering, Qinghai University, Xining 810016, China)
    2(Qinghai Raspberry Agricultural Industrialization Co.Ltd., Xining 812100, China)

Received date: 2023-03-18

  Revised date: 2023-04-17

  Online published: 2024-06-11

Fold

Abstract

In the present study, a raspberry polysaccharide named RPP-5 was isolated and purified using DEAE-Sepharose Fast Flow and Sephadex G-200.Its structural characteristics and immunomodulatory activity were also explored.The structural characteristics of RPP-5 were studied based on the results of high-performance gel permeation chromatography, Fourier transform infrared spectroscopy, and GC-MS. RAW264.7 cells were utilized to evaluate the immunomodulatory activity of RPP-5.The cell viability, contents of NO and inflammatory factors, and mRNA expression levels of inflammatory factors in RAW264.7 cells were measured by CCK-8 assay, microplate method, enzyme-linked immunosorbent assay assays, and real-time quantitative polymerase chain reaction, respectively.Results showed that RPP-5 was composed of arabinose, glucose, rhamnose, galactose, mannose, and xylose, with a molar ratio of 44.5∶21.7∶5.9∶8.8∶5∶4.1.The average molecular weight, peak molecular weight, and number average molecular weight were 7 598 Da, 7 492 Da, and 6 183 Da, respectively.RPP-5 contained 12 types of glycosyl linkages types, among which →4)-Galp-(1→, →4)-Xylp-(1→, Araf-(1→, →4,6)-Glcp-(1→ were the main connection types of sugar residues, accounted for 26.27%, 16.50%, 11.38%, and 10.41%, respectively.RPP-5 significantly increased the viability of RAW264.7 cells, increased the content of NO, IL-6, IL-1β, and TNF-α, and promoted the expression of IL-6, IL-1β, and TNF-α mRNA in RAW264.7 cells.In conclusion, RPP-5 was a homogeneous acidic heteropolysaccharide with a complex multi-branched structure and showed significant immune-enhancing activity.It could be used as an immunomodulator in functional foods, providing theoretical support for the structural characterization and immunological activity studies of raspberry polysaccharides.

Key words： raspberry; polysaccharide; structural characteristics; immunomodulatory activity; RAW264.7 cell

Cite this article

YIN Xingxing , YANG Yongjing , LU Jie , WANG Xuehong , CHAO Mu . Structural characteristics and immunomodulatory activity of raspberry polysaccharide RPP-5[J]. Food and Fermentation Industries, 2024 , 50(9) : 139 -147 . DOI: 10.13995/j.cnki.11-1802/ts.035511

References

[1] 伍芳芳. 猴头菇多糖的结构表征、免疫调节活性及其机理研究[D].广州:华南理工大学, 2018.
WU F F.Structural characterization, immunomodulatory activities and underlying mechanism of Hericium erinaceus polysaccharide[D].Guangzhou:South China University of Technology, 2018.
[2] 董洲. 野葛根多糖的提取、分离纯化、结构鉴定及对小鼠巨噬细胞RAW264.7的免疫调节活性研究[D].广州:华南理工大学, 2018.
DONG Z.Extraction, isolation, purification, structural characterization and immunomodulatory activity in RAW264.7 cells of the polysaccharides from Pueraria lobate (Willd.) Ohwi root[D].Guangzhou:South China University of Technology, 2018.
[3] CORDEIRO CAILLOT A R, DE LACERDA BEZERRA I, PALHARES L C G F, et al.Structural characterization of blackberry wine polysaccharides and immunomodulatory effects on LPS-activated RAW 264.7 macrophages[J].Food Chemistry, 2018, 257:143-149.
[4] GUO W W, ZHANG Q X, DU Y, et al.Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling[J].Chinese Herbal Medicines, 2022, 14(1):90-96.
[5] KAKAR M U, KAKAR I U, MEHBOOB M Z, et al.A review on polysaccharides from Artemisia sphaerocephala Krasch seeds, their extraction, modification, structure, and applications[J].Carbohydrate Polymers, 2021, 252:117113.
[6] 阿荣. 燕麦醪糟多糖的分离纯化、结构鉴定及其免疫活性的研究[D].呼和浩特:内蒙古农业大学, 2019.
A R.The studies on the purification, structure identification and its’s immunity activity of polysaccharides isolated from oat Lao-chao[D].Hohhot:Inner Mongolia Agricultural University, 2019.
[7] 白天凯, 田静, 闫滨.7种国内上市多糖药物临床应用及作用机制研究进展[J].世界科学技术-中医药现代化, 2021, 23(10):3670-3680.
BAI T K, TIAN J, YAN B.Research progress of clinical application and mechanism of action of seven domestic listed polysaccharide drugs[J].Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 2021, 23(10):3670-3680.
[8] 冯荦荦, 王锐, 董兆斌, 等.欧李多糖的制备、结构表征及免疫调节活性研究[J].天然产物研究与开发, 2022, 34(5):739-749.
FENG L L, WANG R, DONG Z B, et al.Preparation, structure characterization and immunoregulatory activity of Cerasus humilis polysaccharide[J].Natural Product Research and Development, 2022, 34(5):739-749.
[9] 王晶, 乔洁, 裴新云, 等.阿魏侧耳胞外多糖分离纯化及其免疫活性研究[J].食品与发酵工业, 2021, 47(18):127-134.
WANG J, QIAO J, PEI X Y, et al.Purification and immunomodulation activity of exopolysaccharides from Pleurotus ferulae Lanzi[J].Food and Fermentation Industries, 2021, 47(18):127-134.
[10] 刘思美, 赵鹏, 张婷婷, 等.地黄硒多糖的合成、表征及免疫活性分析[J].中国中药杂志, 2022, 47(11):2938-2946.
LIU S M, ZHAO P, ZHANG T T, et al.Synthesis, characterization, and immunological activity of Rehmannia glutinosa seleno-polysaccharides[J].China Journal of Chinese Materia Medica, 2022, 47(11):2938-2946.
[11] XU G Y, LIAO A M, HUANG J H, et al.Evaluation of structural, functional, and anti-oxidant potential of differentially extracted polysaccharides from potatoes peels[J].International Journal of Biological Macromolecules, 2019, 129:778-785.
[12] 吴媛媛. 红树莓多糖分离纯化及降血糖作用研究初探[D].哈尔滨:哈尔滨商业大学, 2018.
WU Y Y.Separation purification and hypolipidemic preliminary exploration effect of polysaccharides from red raspberry[D].Harbin:Harbin University of Commerce, 2018.
[13] 孙玉珍, 方毅, 于晓红, 等.红树莓生物活性成分及药理作用研究进展[J].中医药学报, 2017, 45(5):106-109.
SUN Y Z, FANG Y, YU X H, et al.Research progress on bioactive components and pharmacological effects of raspberry[J].Acta Chinese Medicine and Pharmacology, 2017, 45(5):106-109.
[14] ZHANG T T, LU C L, JIANG J G, et al.Bioactivities and extraction optimization of crude polysaccharides from the fruits and leaves of Rubus chingii Hu[J].Carbohydrate Polymers, 2015, 130:307-315.
[15] XU W, ZHAO M, FU X Y, et al.Molecular mechanisms underlying macrophage immunomodulatory activity of Rubus chingii Hu polysaccharides[J].International Journal of Biological Macromolecules, 2021, 185:907-916.
[16] YANG Y J, XU H M, SUO Y R.Raspberry pulp polysaccharides inhibit tumor growth via immunopotentiation and enhance docetaxel chemotherapy against malignant melanoma in vivo[J].Food & Function, 2015, 6(9):3022-3034.
[17] YU Z Y, LIU L, XU Y Q, et al.Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits[J].Carbohydrate Polymers, 2015, 132:180-186.
[18] YANG Y J, YIN X X, ZHANG D J, et al.Isolation, structural characterization and macrophage activation activity of an acidic polysaccharide from raspberry pulp[J].Molecules, 2022, 27(5):1674.
[19] TABARSA M, YOU S G, ABEDI M, et al.The activation of RAW264.7 murine macrophage and natural killer cells by glucomannogalactan polysaccharides from Tornabea scutellifera[J].Carbohydrate Polymers, 2019, 219:368-377.
[20] WANG H M, WANG X L, LI Y, et al.Structural properties and in vitro and in vivo immunomodulatory activity of an Arabinofuranan from the fruits of Akebia quinata[J].Carbohydrate Polymers, 2021, 256:117521.
[21] ZENG Y J, YANG H R, WU X L, et al.Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation[J].International Journal of Biological Macromolecules, 2019, 137:568-575.
[22] ZHOU Y, WANG S C, FENG W S, et al.Structural characterization and immunomodulatory activities of two polysaccharides from Rehmanniae Radix Praeparata[J].International Journal of Biological Macromolecules, 2021, 186:385-395.
[23] ZHOU W T, ZHAO Y, YAN Y M, et al.Antioxidant and immunomodulatory activities in vitro of polysaccharides from bee collected pollen of Chinese wolfberry[J].International Journal of Biological Macromolecules, 2020, 163:190-199.
[24] 刘晓飞, 马京求, 侯艳, 等.发芽糙米多糖的结构解析及降糖活性分析[J].中国食品学报, 2021, 21(8):265-274.
LIU X F, MA J Q, HOU Y, et al.Structure analysis and hypoglycemic activity of germinated brown rice polysaccharides[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(8):265-274.
[25] SAHRAGARD N, JAHANBIN K.Structural elucidation of the main water-soluble polysaccharide from Rubus anatolicus roots[J].Carbohydrate Polymers, 2017, 175:610-617.
[26] KE H H, BAO T, CHEN W.Polysaccharide from Rubus chingii Hu affords protection against palmitic acid-induced lipotoxicity in human hepatocytes[J].International Journal of Biological Macromolecules, 2019, 133:1063-1071.
[27] WU D M, CHEN S G, YE X Q, et al.Enzyme-extracted raspberry pectin exhibits a high-branched structure and enhanced anti-inflammatory properties than hot acid-extracted pectin[J].Food Chemistry, 2022, 383:132387.
[28] QIN L, CHEN S, XIE L M, et al.Mechanisms of RAW264.7 macrophages immunomodulation mediated by polysaccharide from mung bean skin based on RNA-seq analysis[J].Food Research International, 2022, 154:111017.
[29] HUO J Y, WU J H, ZHAO M M, et al.Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression[J].Food Chemistry, 2020, 330:127257.
[30] YUE H, XU Q Q, BIAN G Y, et al.Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum[J].International Journal of Biological Macromolecules, 2020, 155:853-860.
[31] XIE L M, HUANG Z B, MENG H, et al.Immunomodulation effect of polysaccharides from liquid fermentation of Monascus purpureus 40269 via membrane TLR-4 to activate the MAPK and NF-κB signaling pathways[J].International Journal of Biological Macromolecules, 2022, 201:480-491.
[32] PAN Q T, SUN Y L, LI X Y, et al.Extraction, structural characterization, and antioxidant and immunomodulatory activities of a polysaccharide from Notarchus leachii freeri eggs[J].Bioorganic Chemistry, 2021, 116:105275.
[33] SUN Y, ZHANG Z P, CHENG L, et al.Polysaccharides confer benefits in immune regulation and multiple sclerosis by interacting with gut microbiota[J].Food Research International, 2021, 149:110675.
[34] MENG X, LIANG H B, LUO L X.Antitumor polysaccharides from mushrooms:A review on the structural characteristics, antitumor mechanisms and immunomodulating activities[J].Carbohydrate Research, 2016, 424:30-41.
[35] LO T C T, JIANG Y H, CHAO A L J, et al.Use of statistical methods to find the polysaccharide structural characteristics and the relationships between monosaccharide composition ratio and macrophage stimulatory activity of regionally different strains of Lentinula edodes[J].Analytica Chimica Acta, 2007, 584(1):50-56.
[36] ZHAN Q P, WANG Q, LIN R G, et al.Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit[J].International Journal of Biological Macromolecules, 2020, 145:1080-1090.
[37] DING X, ZHU F S, GAO S G.Purification, antitumour and immunomodulatory activity of water-extractable and alkali-extractable polysaccharides from Solanum nigrum L[J].Food Chemistry, 2012, 131(2):677-684.
[38] 崔连杰. 黄芪活性多糖APS-II免疫调控机制及其酶解寡糖片段的结构和活性研究[D].太原:山西大学, 2021.
CUI L J.Immunoregulatory mechanism of APS-II, an active polysaccharide from Astragalus membranaceus, and study on the structure and activity of its enzymatic oligosaccharide fragment[D].Taiyuan:Shanxi University, 2021.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.