Construction and application of Spirulina protein isolate-hyaluronic acid emulsion hydrogel

  • CHEN Linqian ,
  • LI Yunxing ,
  • MAO Yi ,
  • QIU Xiaoyuan ,
  • YANG Cheng ,
  • SUN Yajuan
Expand
  • (Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China)

Received date: 2024-01-11

  Revised date: 2024-02-29

  Online published: 2024-07-11

Abstract

Protein-polysaccharide emulsion hydrogel, as an encapsulation system of active substances, has the advantages of high loading capacity, biocompatibility and stability.In this work, Spirulina protein isolate (SPI) and hyaluronic acid (HA) were used as emulsifier and gel matrix to prepare SPI emulsions, SPI-HA emulsions, and emulsion hydrogels with various oil phase volume fractions.The microstructure and droplet sizes of the emulsions were characterized.The effect of oil phase volume fractions on the formation of emulsion hydrogel was analyzed, and the protective effect of emulsion hydrogel on β-carotene under high temperature was studied.The results showed that the droplet sizes of SPI emulsion decreased obviously with the introduction of HA.The structure of emulsion hydrogel became much denser with the increase of oil phase volume fraction.When the oil phase volume fraction was 60%, the yield stress, storage modulus and apparent viscosity of emulsion hydrogel were higher than those of SPI emulsion and SPI-HA emulsion.The retention rate of β-carotene after storage at 70 ℃ and 8 h was as high as (78.83±2.75)%. In summary, SPI-HA emulsion hydrogels had good application in the field of food.

Cite this article

CHEN Linqian , LI Yunxing , MAO Yi , QIU Xiaoyuan , YANG Cheng , SUN Yajuan . Construction and application of Spirulina protein isolate-hyaluronic acid emulsion hydrogel[J]. Food and Fermentation Industries, 2024 , 50(12) : 39 -45 . DOI: 10.13995/j.cnki.11-1802/ts.038547

References

[1] BHATT T, PATEL K.Carotenoids:Potent to prevent diseases review[J].Natural Products and Bioprospecting, 2020, 10(3):109-117.
[2] 李超, 贾炳玉, 高民, 等.β-胡萝卜素的生物学作用及其机理[J].动物营养学报, 2018, 30(8):2931-2937.
LI C, JIA B Y, GAO M, et al.Biological functions and its mechanisms of β-carotene[J].Chinese Journal of Animal Nutrition, 2018, 30(8):2931-2937.
[3] 郭静, 孙晓琳, 潘思轶.环境因素对β-胡萝卜素复合纳米粒子稳定性的影响[J].食品科学, 2022, 43(16):90-97.
GUO J, SUN X L, PAN S Y.Effect of environmental conditions on the stability of β-carotene nanoparticles[J].Food Science, 2022, 43(16):90-97.
[4] ROOHINEJAD S, OEY I, WEN J Y, et al.Formulation of oil-in-water β-carotene microemulsions:Effect of oil type and fatty acid chain length[J].Food Chemistry, 2015, 174:270-278.
[5] TAN C, ZHANG Y T, ABBAS S, et al.Modulation of the carotenoid bioaccessibility through liposomal encapsulation[J].Colloids and Surfaces B:Biointerfaces, 2014, 123:692-700.
[6] 瓦文强, 秦娟娟, 杨敏, 等.酪蛋白胶束乳液凝胶性质及其在大黄素负载中的应用[J].食品与发酵工业, 2023, 49(1):132-139.
WA W Q, QIN J J, YANG M, et al.Properties of emulsion gel based on casein micelles and its application in emodin loading[J].Food and Fermentation Industries, 2023, 49(1):132-139.
[7] DICKINSON E.Emulsion gels:The structuring of soft solids with protein-stabilized oil droplets[J].Food Hydrocolloids, 2012, 28(1):224-241.
[8] 刁小琴, 李曦, 孙薇婷, 等.乳液凝胶的构建及应用研究进展[J].食品安全质量检测学报, 2022, 13(4):1036-1043.
DIAO X Q, LI X, SUN W T, et al.Research progress in the fabrication and application of emulsion gels[J].Journal of Food Safety and Quality, 2022, 13(4):1036-1043.
[9] LIANG L, LEUNG SOK LINE V, REMONDETTO G E, et al.In vitro release of α-tocopherol from emulsion-loaded β-lactoglobulin gels[J].International Dairy Journal, 2010, 20(3):176-181.
[10] GEREMIAS-ANDRADE I M, SOUKI N P D B G, MORAES I C F, et al.Rheological and mechanical characterization of curcumin-loaded emulsion-filled gels produced with whey protein isolate and xanthan gum[J].LWT, 2017, 86:166-173.
[11] TANG Z Q, HE H C, ZHU L, et al.A general protein unfolding-chemical coupling strategy for pure protein hydrogels with mechanically strong and multifunctional properties[J].Advanced science, 2022, 9(5):e2102557.
[12] TIAN R, QIU X Y, YUAN P Y, et al.Fabrication of self-healing hydrogels with on-demand antimicrobial activity and sustained biomolecule release for infected skin regeneration[J].ACS Applied Materials & Interfaces, 2018, 10(20):17018-17027.
[13] LIU F G, LIANG X P, YAN J, et al.Tailoring the properties of double-crosslinked emulsion gels using structural design principles:Physical characteristics, stability, and delivery of lycopene[J].Biomaterials, 2022, 280:121265.
[14] BÖCKER L, BERTSCH P, WENNER D, et al.Effect of Arthrospira platensis microalgae protein purification on emulsification mechanism and efficiency[J].Journal of Colloid and Interface Science, 2021, 584:344-353.
[15] CHEN S, HAN Y, H WANG Y Q, et al.Zein-hyaluronic acid binary complex as a delivery vehicle of quercetagetin:Fabrication, structural characterization, physicochemical stability and in vitro release property[J].Food Chemistry, 2019, 276:322-332.
[16] KOVÁCS A N, VARGA N, JUHÁSZ Á, et al.Serum protein-hyaluronic acid complex nanocarriers:Structural characterisation and encapsulation possibilities[J].Carbohydrate Polymers, 2021, 251:117047.
[17] DING J J, LI Y X, WANG Q B, et al.Pickering high internal phase emulsions with excellent UV protection property stabilized by Spirulina protein isolate nanoparticles[J].Food Hydrocolloids, 2023, 137:108369.
[18] LU Y, MAO L K, CUI M N, et al.Effect of the solid fat content on properties of emulsion gels and stability of β-carotene[J].Journal of Agricultural and Food Chemistry, 2019, 67(23):6466-6475.
[19] GOMES A, COSTA A L R, CUNHA R L.Impact of oil type and WPI/Tween 80 ratio at the oil-water interface:Adsorption, interfacial rheology and emulsion features[J].Colloids and Surfaces. B, Biointerfaces,2018, 164:272-280.
[20] WANG N Z, ZHAO X, JIANG Y Q, et al.Enhancing the stability of oil-in-water emulsions by non-covalent interaction between whey protein isolate and hyaluronic acid[J].International Journal of Biological Macromolecules, 2023, 225:1085-1095.
[21] 吴彤, 冯进, 黄午阳, 等.牛蒡果胶多糖/玉米醇溶蛋白复合颗粒稳定的Pickering乳液构建及对姜黄素的递送功效[J].食品科学, 2023, 44(14):37-46.
WU T, FENG J, HUANG W Y, et al.Fabrication of Pickering emulsions stabilized by burdock rhamnogalacturonan I (RG-1)-type pectin/zein composite particles and their utilization for the delivery of curcumin[J].Food Science, 2023, 44(14):37-46.
[22] 王小庆, 任健.不同油相比例对黑豆分离蛋白乳液凝胶特性的影响[J].中国油脂, 2018, 43(1):103-106.
WANG X Q, REN J.Influences of oil volume fraction on properties of black bean protein isolate emulsion gel[J].China Oils and Fats, 2018, 43(1):103-106.
[23] KIM H S, MASON T G.Advances and challenges in the rheology of concentrated emulsions and nanoemulsions[J].Advances in Colloid and Interface Science, 2017, 247:397-412.
[24] LIU H, XU X M, GUO SH D.Rheological, texture and sensory properties of low-fat mayonnaise with different fat mimetics[J].LWT-Food Science and Technology, 2007, 40(6):946-954.
[25] 吕静雯, 曹杨, 刘潇, 等.卵磷脂对乳清分离蛋白乳液性质和乳液凝胶结构特性及其所负载β-胡萝卜素的影响[J].食品安全质量检测学报, 2023, 14(7):164-172.
LYU J W, CAO Y, LIU X, et al.Effects of lecithin on the emulsion properties, emulsion gel structure characteristics and its loaded β-carotene of whey isolate protein[J].Journal of Food Safety & Quality, 2023, 14(7):164-172.
Outlines

/