Food and Fermentation Industries >

2024 , Vol. 50 >Issue 12: 135 - 141

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036805

Analysis of microbial diversity in swollen irradiated chicken feet based on pure culture method and high-throughput sequencing technology

  • JIA Xin ,
  • GAO Peng ,
  • ZHOU Rui ,
  • XU Pan ,
  • WANG Dan ,
  • HUANG Min ,
  • MENG Ailian ,
  • ZHANG Yanjun ,
  • WU Geyang
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  • 1(School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)
    2(Sichuan Institute of Atomic Energy, Chengdu 610101, China)
    3(Irradiation preservation Key Laboratory of Sichuan Province, Chengdu 610101, China)

Received date: 2023-07-17

  Revised date: 2023-08-23

  Online published: 2024-07-11

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Abstract

To investigate the microbial diversity of vacuum-packaged swollen irradiated chicken feet, the microbial diversity was analyzed in this study using the pure culture method and high-throughput sequencing technology.The pure culture approach revealed the identification of 13 strains, comprising 4 strains of Lactiplantibacillus plantarum, 3 strains of Lactobacillus brevis, 1 strain of Stenotrophomonas maltophilia, 3 strains of Leuconostoc fallax, and 2 strains of Candida tropicalis.The results of high-throughput sequencing showed that Lactobacillus exhibited the highest relative abundance (averaging at 97.30%) and was the dominant bacterium in the swollen chicken feet.Additionally, gas production verification experiments revealed that L.brevis and L.fallax could cause bag swelling.The results of this study can provide a certain reference for mastering the microbial contamination and community composition of swollen irradiated chicken feet products, and provide a solid theoretical foundation for the subsequent control of microbial contamination and enhancement of product quality.

Key words： swollen; irradiated chicken feet; pure culture method; high throughput sequencing; microbial diversity

Cite this article

JIA Xin , GAO Peng , ZHOU Rui , XU Pan , WANG Dan , HUANG Min , MENG Ailian , ZHANG Yanjun , WU Geyang . Analysis of microbial diversity in swollen irradiated chicken feet based on pure culture method and high-throughput sequencing technology[J]. Food and Fermentation Industries, 2024 , 50(12) : 135 -141 . DOI: 10.13995/j.cnki.11-1802/ts.036805

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