Food and Fermentation Industries >

2024 , Vol. 50 >Issue 12: 227 - 233

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036916

Isolation of efficient nucleoside-degrading lactic acid bacteria from traditional pickles and their probiotic properties

  • LI Jie ,
  • ZHANG Pengxia ,
  • ZHOU Jian ,
  • HE Min ,
  • PENG Jingxian ,
  • BAO Yan ,
  • SONG Lixin
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  • 1(School of Public Health, Jiamusi University, Jiamusi 154000, China)
    2(School of Basic Medical Sciences, Jiamusi University, Jiamusi 154000, China)
    3(Baotou Disease Prevention Control Center, Baotou 014030, China)
    4(School of Public Health, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014060, China)

Received date: 2023-07-28

  Revised date: 2023-08-08

  Online published: 2024-07-11

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Abstract

Hyperuricemia is a metabolic disease caused by uric acid metabolism disorder.Lactic acid bacteria as an important probiotic could ameliorate hyperuricemia in multiple ways.The study aimed to isolate lactic acid bacteria with capable to degrade nucleosides efficiently from pickles.The nucleoside degradation ability of the screened strains was analysed by using high performance liquid chromatography (HPLC), then the xanthine oxidase inhibition by strains, environmental tolerance in vitro and antibiotic susceptibility were also tested. Results showed that the degradation rate of inosine and guanosine by lactobacillus brevis SLlac-18 were 97.81% and 97.89%, respectively, and xanthine oxidase inhibition of strains were also existed.In addition, the survival rate of SLlac-18 was 96% at pH 2.0, 96.33% at 3 g/L bile salt for 5 h, and 96.33% at gastrointestinal fluid for 5 h.SLlac-18 was also highly tolerant to the treatment of gastrointestinal fluids with a survival rate of more than 90%, and was susceptible to four common antibiotics such as erythromycin and ampicillin.lactobacillus brevis SLlac-18 has the potential function to degrade uric acid, which can be applied to the prevention and treatment of hyperuricemia, that has certain application value.

Key words： hyperuricaemia; nucleosides; lactic acid bacteria; pickle; probiotics

Cite this article

LI Jie , ZHANG Pengxia , ZHOU Jian , HE Min , PENG Jingxian , BAO Yan , SONG Lixin . Isolation of efficient nucleoside-degrading lactic acid bacteria from traditional pickles and their probiotic properties[J]. Food and Fermentation Industries, 2024 , 50(12) : 227 -233 . DOI: 10.13995/j.cnki.11-1802/ts.036916

References

[1] JUNG S W, KIM S M, KIM Y G, et al.Uric acid and inflammation in kidney disease[J].American Journal of Physiology, 2020, 318(6):F1327-F1340.
[2] CHOI H K, LIU S M, CURHAN G.Intake of purine-rich foods, protein, and dairy products and relationship to serum levels of uric acid:The Third National Health and Nutrition Examination Survey[J].Arthritis and Rheumatism, 2005, 52(1):283-289.
[3] CHOI H K, ATKINSON K, KARLSON E W, et al.Purine-rich foods, dairy and protein intake, and the risk of gout in men[J].The New England Journal of Medicine,2004, 350(11):1093-1103.
[4] PASCART T, RICHETTE P.Investigational drugs for hyperuricemia, an update on recent developments[J].Expert Opinion on Investigational Drugs, 2018, 27 (5):437-444.
[5] 牛春华, 肖茹雪, 赵子健, 等.植物乳杆菌UA149的降尿酸作用[J].现代食品科技, 2020, 36(2):1-6;217.
NIU C H, XIAO R X, ZHAO Z J, et al.Serum uric acid lowering effect of Lactobacillus plantarum UA149 on hyperuricemic rats[J].Modern Food Science and Technology, 2020, 36(2):1-6;217.
[6] 呼静, 崔鹏月, 双全, 等.高效降尿酸乳酸菌的筛选及其益生特性研究[J].食品与发酵工业, 2022, 48(16):150-155.
HU J, CUI P Y, SHUANG Q, et al.Screening of lactic acid bacteria with high ability of uric acid-lowering and evaluation of its probiotic properties[J].Food and Fermentation Industries, 2022, 48(16):150-155.
[7] 王家彬, 潘力.降解核苷作用乳酸菌的筛选及其潜在降尿酸功能[J].食品科学, 2022, 43(10):199-206.
WANG J B, PAN L.Screening and uric acid-lowering potential of nucleoside-degrading lactic acid bacteria[J].Food Science, 2022, 43(10):199-206.
[8] WANG H N, MEI L, DENG Y, et al.Lactobacillus brevis DM9218 ameliorates fructose-induced hyperuricemia through inosine degradation and manipulation of intestinal dysbiosis[J].Nutrition, 2019, 62:63-73.
[9] LEE Y, WERLINGER P, SUH J W, et al.Potential probiotic Lacticaseibacillus paracasei MJM60396 prevents hyperuricemia in a multiple way by absorbing purine, suppressing xanthine oxidase and regulating urate excretion in mice[J].Microorganisms, 2022, 10(5):851.
[10] KUO Y W, HSIEH S H, CHEN J F, et al.Lactobacillus reuteri TSR332 and Lactobacillus fermentum TSF331 stabilize serum uric acid levels and prevent hyperuricemia in rats[J].PeerJ,2021, 9:e11209.
[11] TSUBOI H, KANEKO N, SATOU A, et al.Lactic acid bacteria having action of lowering blood uric acid level:US8460918,918[P].2013-06-11.
[12] 刘玉兵. 光质对小型大白菜产量与品质形成的生理机制研究[D].长沙:湖南农业大学, 2021.
LIU Y B.Physiological mechanism of light quality on yield and quality formation of mini Chinese cabbage [D].Changsha:Hunan Agricultural University, 2021.
[13] 李艳莉. 酸奶及发酵剂中分离乳酸菌药物敏感性研究[D].广州:华南理工大学, 2015.
LI Y L.Antimicrobial susceptibilities of lactic acid bacteria isolated from yogurts and starter culture[D].Guangzhou:South China University of Technology, 2015.
[14] CHOI H K.A prescription for lifestyle change in patients with hyperuricemia and gout[J].Current Opinion in Rheumatology, 2010, 22(2):165-172.
[15] HAO S J, ZHANG C L, SONG H Y.Natural products improving hyperuricemia with hepatorenal dual effects[J].Evidence Based Complementary and Alternative Medicine:ECAM, 2016, 2016:7390504.
[16] WANG J, CHEN Y, ZHONG H, et al.The gut microbiota as a target to control hyperuricemia pathogenesis:Potential mechanisms and therapeutic strategies[J].Critical Reviews in Food Science and Nutrition,2022, 62(14):3979-3989.
[17] 黄佳豪, 李先平, 赵军英, 等.益生菌缓解高尿酸血症作用机制研究进展[J].食品科学, 2023, 44(23):282-292.
HUANG J H, LI X P, ZHAO J Y, et al.Progress on the mechanism of action of probiotics in alleviating hyperuricaemia[J].Food Science, 2023, 44(23):282-292.
[18] WU Y, YE Z, FENG P Y, et al.Limosilactobacillus fermentum JL-3 isolated from "Jiangshui" ameliorates hyperuricemia by degrading uric acid[J].Gut Microbes, 2021, 13(1):1-18.
[19] GARCIA-ARROYO F E, GONZAGA G, MUNOZ-JIMENEZ I, et al.Probiotic supplements prevented oxonic acid-induced hyperuricemia and renal damage[J].PLoS One, 2018, 13(8):e0202901.
[20] 王垚, 李文静, 鲁茂林, 等.不同来源乳酸菌核苷降解能力研究[J].中国乳品工业, 2021, 49(5):4-7;14.
WANG Y, LI W J, LU M L, et al.Study on the nucleoside degradation ability of lactic acid bacteria from different sources[J].China Dairy Industry, 2021, 49(5):4-7;14.
[21] 蚁硕钊, 李啟彬, 房志家, 等.海洋源降解核苷乳酸菌的体外筛选、鉴定及其益生特性研究[J].食品与发酵工业, 2022, 48(11):87-94.
YI S Z, LI Q B, FANG Z J, et al.In vitro screening, identification and probiotic characteristics of marine source lactic acid bacteria with purine nucleoside degrading ability[J].Food and Fermentation Industries, 2022, 48(11):87-94.
[22] XIAO Y X, ZHANG C X, ZENG X L, et al.Microecological treatment of hyperuricemia using Lactobacillus from pickles[J].BMC Microbiology, 2020, 20(1):195.
[23] MANDAL A K, MOUNT D B.The molecular physiology of uric acid homeostasis[J].Annual Review of Physiology, 2015, 77:323-345.
[24] 任宇杰, 单成俊, 王英, 等.降解嘌呤核苷乳酸菌的筛选及益生特性探索[J].微生物学通报, 2023, 50(2):541-552.
REN Y J, SHAN C J, WANG Y, et al.Screening of purine nucleoside-degrading lactic acid bacteria and exploration of its probiotic properties[J].Microbiology China, 2023, 50(2):541-552.
[25] 麻菊美, 赵伟睿, 胡升, 等.高效降解嘌呤核苷乳酸菌的筛选及特性研究[J].食品与生物技术学报, 2019, 38(4):8-14.
MA J M, ZHAO W R, HU S, et al.Isolation of a lactic acid bacterium with high ability of purine nucleoside-decomposing and evaluation of its probiotic properties[J].Journal of Food Science and Biotechnology, 2019, 38(4):8-14.
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