Food and Fermentation Industries >

2024 , Vol. 50 >Issue 11: 1 - 8

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038530

Physiological mechanism of Bacillus cereus AR1002 inhibiting growth of Aspergillus flavus based on transcriptomic analysis

  • TANG Xiaoqian ,
  • ZHANG Jingbo ,
  • GAO Shang ,
  • JIANG Jun ,
  • ZHANG Qi ,
  • LI Peiwu
Expand
  • 1(Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)
    2(Oil Quality Supervision, Inspection and Testing Center, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China)
    3(Oil Product Quality and Safety Risk Assessment Laboratory of Ministry of Agriculture and Rural Affairs (Wuhan), Wuhan 430062, China)
    4(Key Laboratory of Oil Crop Biology, Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China)
    5(Institute of Food Safety, Hubei University, Wuhan 430062, China)
    6(College of Agriculture, Zhengzhou University, Zhengzhou 450000, China)
    7(Yangtze University, Jingzhou 434025, China)
    8(Xianghu Laboratory, Hangzhou 311231, China)
    9(Hubei Hongshan Laboratory, Wuhan 430070, China)

Received date: 2024-01-11

  Revised date: 2024-02-06

  Online published: 2024-07-12

Fold

Abstract

Aspergillus flavus is susceptible to contaminating agricultural food, especially the oilcrops, aflatoxin is the secondary metabolite of A. flavus, which is seriously harmful to human health.Preventing and controlling aflatoxin contamination has become an urgent international problem.This study aimed to investigate the physiological and molecular mechanism of Bacillus cereus AR1002 in inhibiting the growth and pollution of A. flavus.In this study, the metabolites prepared by AR1002 were used to treat A. flavus and then identify its growth and sporulation phenotypes.At the same time, the antibacterial mechanism of AR1002 was studied by methods of microstructure and transcriptomic analysis.The metabolites of B. cereus AR1002 could significantly inhibit the mycelium growth, dry matter accumulation and spore number of A. flavus, with inhibition rates of 24.73%, 65.00%, and 98.80%, respectively.In addition, AR1002 metabolites can disturb the glycerophosphate metabolism of A. flavus by inhibiting the expression of key genes such as glpA and AYR1.At the same time, the expression of SEC61A, RAD23, ATP13A1, UBE2G2 and other key genes is inhibited to regulate the processing of proteins in the endoplasmic reticulum.AR1002 metabolites also inhibited homologous recombination and non-homologous end-linking processes of A. flavus by down-regulating genes related to DNA repair mechanisms such as RAD51 and RAD54B.Finally, the aim of inhibiting the growth and sporulation of A. flavus was achieved.Results indicated that the metabolites of B. cereus AR1002 were expected to be applied in practical production to solve the problem of A. flavus contamination.

Key words： Bacillus cereus; metabolite; aflatoxins; transcriptomics; inhibitory mechanism

Cite this article

TANG Xiaoqian , ZHANG Jingbo , GAO Shang , JIANG Jun , ZHANG Qi , LI Peiwu . Physiological mechanism of Bacillus cereus AR1002 inhibiting growth of Aspergillus flavus based on transcriptomic analysis[J]. Food and Fermentation Industries, 2024 , 50(11) : 1 -8 . DOI: 10.13995/j.cnki.11-1802/ts.038530

References

[1] 郑爱芳, 何欢, 高晔, 等.一株防治花生采后黄曲霉污染的拮抗菌的筛选及其防控效果[J].中国油脂, 2024, 49(2):56-62.
ZHENG A F, HE H, GAO Y, et al.Screening of biocontrol bacteria against peanut postharvest Aspergillus flavus contamination and its prevention effect[J].China Oils and Fats, 2024, 49(2):56-62.
[2] 杜瑞焕, 高素艳, 张谊, 等.花生黄曲霉毒素污染拮抗菌B25-5的筛选、鉴定及控制效果[J].中国粮油学报, 2018, 33(8):64-68.
DU R H, GAO S Y, ZHANG Y, et al.Screening, identification and control effect of aflatoxin antagonistic bacteria B25-5 in peanut[J].Journal of the Chinese Cereals and Oils Association, 2018, 33(8):64-68.
[3] 姚彦坡, 丁丹, 张友青, 等.玉米黄曲霉毒素污染生防菌筛选及菌株B42-3抗菌活性研究[J].中国粮油学报, 2018, 33(3):84-88.
YAO Y P, DING D, ZHANG Y Q, et al.Screening of maize endophytic antagonists against Aspergillus flavus and activities research of antagonistic strain B42-3[J].Journal of the Chinese Cereals and Oils Association, 2018, 33(3):84-88.
[4] 陈兰, 谢永丽, 吴晓晖, 等.4株促紫花苜蓿生长的芽孢杆菌分子鉴定及其生物活性分析[J].西北农业学报, 2023, 32(2):212-221.
CHEN L, XIE Y L, WU X H, et al.Molecular identification and bioactivity analysis of four Bacillus strains promoting the growth of alfalfa[J].Acta Agriculturae Boreali-occidentalis Sinica, 2023, 32(2):212-221.
[5] 周瑚, 刘剑波, 谭陈勇, 等.蜡样芽孢杆菌YN917对水稻稻瘟病菌的拮抗机理及应用[J].农药, 2023, 62(3):213-216.
ZHOU H, LIU J B, TAN C Y, et al.Antagonistic mechanisms and biocontrol effect of Bacillus cereus strain YN917 upon Magnaporthe oryzae[J].Agrochemicals, 2023, 62(3):213-216.
[6] 王卓妮, 覃艮红, 王丽, 等.草莓病害拮抗细菌的筛选及其对草莓褐色叶斑病的防效[J].中国蔬菜, 2023(2):63-71.
WANG Z N, QIN G H, WANG L, et al.Screening of antagonistic bacteria from strawberry and their biocontrol effect against tan-brown leaf spot of strawberry[J].China Vegetables, 2023(2):63-71.
[7] 朱海云, 马瑜, 柯杨, 等.抗猕猴桃细菌性溃疡病蜡样芽孢杆菌MA23培养基及发酵条件优化[J].中国农学通报, 2021, 37(7):112-118.
ZHU H Y, MA Y, KE Y, et al.Optimization of culture medium and fermentation parameters of Bacillus cereus MA23 antagonistic to kiwifruit canker[J].Chinese Agricultural Science Bulletin, 2021, 37(7):112-118.
[8] 潘晓梅, 李昭煜, 石晓玲, 等.番茄灰霉生防菌XF的筛选、鉴定及生防因子的初步探索[J].西北农业学报, 2019, 28(11):1888-1895.
PAN X M, LI Z Y, SHI X L, et al.Screening, identification and biocontrol effects of antagonistic bacterial strain XF and preliminary exploration of biocontrol factors[J].Acta Agriculturae Boreali-occidentalis Sinica, 2019, 28(11):1888-1895.
[9] 王明清, 张初署, 于丽娜, 等.降解黄曲霉毒素B1芽孢杆菌的筛选与鉴定[J].山东农业科学, 2018, 50(11):71-75.
WANG M Q, ZHANG C S, YU L N, et al.Screening and identification of Bacillus strain for degradation of aflatoxin B1[J].Shandong Agricultural Sciences, 2018, 50(11):71-75.
[10] 王英, 黄旭辉, 李冬梅, 等.磷脂氧化降解机制及调控技术研究进展[J/OL].食品科学, 2023:1-16.(2023-10-11).https://kns.cnki.net/kcms/detail/11.2206.TS.20231010.0914.020.html.
WANG Y, HUANG X H, LI D M, et al.Research progress on the mechanism and regulation technology of phospholipid oxidative degradation[J/OL].Food Science, 2023:1-16.(2023-10-11).https://kns.cnki.net/kcms/detail/11.2206.TS.20231010.0914.020.html.
[11] 牟亚豪, 刘帼芬, 孙艺菲, 等.内质网蛋白加工通路关键信号分子在镉致胰岛β细胞凋亡过程中的变化研究[J].现代预防医学, 2022, 49(4):711-714;732.
MOU Y H, LIU G F, SUN Y F, et al.Changes in key molecules of protein processing in endoplasmic reticulum during cadmium-induced apoptosis in pancreatic β-cells[J].Modern Preventive Medicine, 2022, 49(4):711-714;732.
[12] 耿喜宁, 芦特, 杜康, 等.不同基因型毛白杨同源重组变异研究[J].遗传, 2021, 43(2):182-199.
GENG X N, LU T, DU K, et al.Variation of homologous recombination in Populus tomentosa with different genotypes[J].Hereditas(Beijing), 2021, 43(2):182-199.
[13] 杨冰娟, 陶睿泽, 林丽, 等.芽孢杆菌抑制玉米茎腐病菌禾谷镰孢菌和拟轮枝镰孢菌的研究进展[J].江苏农业科学, 2023, 51(12):42-49.
YANG B J, TAO R Z, LIN L, et al.Research progress on inhibition effects of Bacillus spp.against maize stalk rot pathogen (Fusarium graminearum & F.verticillioides)[J].Jiangsu Agricultural Sciences, 2023, 51(12):42-49.
[14] 赵晨晨, 焦铸锦, 庞发虎, 等.生防菌R-4的鉴定及其对玉米南方锈病的防效[J].玉米科学, 2017, 25(2):136-141.
ZHAO C C, JIAO Z J, PANG F H, et al.Identification of biocontrol strain R-4 and its efficacy in controlling southern corn rust[J].Journal of Maize Sciences, 2017, 25(2):136-141.
[15] 付克剑, 宋学茹, 杨芳, 等.玉溪雪茄烟发酵霉变病原菌鉴定及生防菌筛选[J].中国烟草科学, 2023, 44(4):41-48.
FU K J, SONG X R, YANG F, et al.Identification of pathogenic fungi and screening for biocontrol bacteria during fermentation of Yuxi cigars[J].Chinese Tobacco Science, 2023, 44(4):41-48.
[16] 赵帅, 田长彦, 史应武, 等.黄瓜枯萎病生防菌HD-087产抗菌物质条件的优化及抑菌作用初探[J].微生物学通报, 2013, 40(5):802-811.
ZHAO S, TIAN C Y, SHI Y W, et al.Fermentation conditions for the biocontrol strain HD-087 against cucumber Fusarium wilt and it’s antibacterial effect[J].Microbiology China, 2013, 40(5):802-811.
[17] 李珅瑀, 贾祥子, 郭君陶, 等.水稻稻瘟病生防细菌5-8的鉴定及其生防机制研究[J].北方农业学报, 2021, 49(3):74-81.
LI S Y, JIA X Z, GUO J T, et al.Identification of rice blast control bacteria 5-8 and its biocontrol mechanism[J].Journal of Northern Agriculture, 2021, 49(3):74-81.
[18] 张立娜. 一株蜡样芽孢杆菌的鉴定及其抑菌物质的分离纯化与性质的研究[D].广州:华南理工大学, 2012.
ZHANG L N.Identiifcation of a Bacillus cereus and isolation and study on its antimicrobial substance[D].Guangzhou:South China University of Technology, 2012.
[19] 张良, 潘滢浩, 袁瑜, 等.基于细胞膜损伤机制的抗菌肽结构优化研究进展[J].中国抗生素杂志, 2023, 48(7):721-727.
ZHANG L, PAN Y H, YUAN Y, et al.Research progress on structure modification of antimicrobial peptides targeting membrane disruption[J].Chinese Journal of Antibiotics, 2023, 48(7):721-727.
[20] 钟振兴, 彭鑫, 孔德新.靶向同源重组修复的抗肿瘤研究进展[J].药学学报, 2020, 55(11):2535-2548.
ZHONG Z X, PENG X, KONG D X.Research progress in targeting homologous recombination repair for tumor therapy[J].Acta Pharmaceutica Sinica, 2020, 55(11):2535-2548.
[21] ASHWORTH A, LORD C J.Synthetic lethal therapies for cancer:What’s next after PARP inhibitors?[J].Nature Reviews.Clinical Oncology, 2018, 15(9):564-576.
[22] KLINAKIS A, KARAGIANNIS D, RAMPIAS T.Targeting DNA repair in cancer:Current state and novel approaches[J].Cellular and Molecular Life Sciences:CMLS, 2020, 77(4):677-703.
[23] 魏雯帆, 郑碧玉, 符传孩.内质网与线粒体接触复合物ERMES在真菌中的结构组成和功能[J].中国科学(生命科学), 2022, 52(1):67-75.
WEI W F, ZHENG B Y, FU C H.The structural organization and functions of the ERMES complex in fungi[J].Scientia Sinica (Vitae), 2022, 52(1):67-75.
[24] 李贞, 陈伟琴.自噬与念珠菌致病及耐药的相关性[J].中国医药导报, 2023, 20(14):43-46;51.
LI Z, CHEN W Q.Correlation between autophagy and Candida pathogenicity and drug resistance[J].China Medical Herald, 2023, 20(14):43-46;51.
[25] 周娜娜, 王禄山, 公维丽, 等.丝状真菌胞外蛋白高效分泌机制的研究进展[J].生物化学与生物物理进展, 2016, 43(1):44-54.
ZHOU N N, WANG L S, GONG W L, et al.Research progress on the efficient secretion mechanism of extracellular proteins in filamentous fungi[J].Progress in Biochemistry and Biophysics, 2016, 43(1):44-54.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.