Effect of oleic acid on structural and physicochemical properties of sarcoplasmic proteins in Scomberomorus niphonius

  • WANG Youjun ,
  • ZHOU Limeijia ,
  • ZHAO Yangmeijin ,
  • AO Chengxiang ,
  • LIU Jingyi ,
  • ZHAO Hui ,
  • LU Hang
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  • 1(College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China)
    2(Liaoning Provincial Key Laboratory of Aquatic Products Processing and Comprehensive Utilization, Dalian 116023, China)
    3(Collaborative Innovation Center for Key Technologies of Marine Food Finishing and Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2023-06-15

  Revised date: 2023-06-30

  Online published: 2024-08-02

Abstract

To investigate the sarcoplasmic protein (SP) induced by oleic acid (OA), different concentrations (0-40 mmol/L) of OA were used to treat sarcoplasmic proteins of Scomberomorus niphonius and the structural and physicochemical properties of SP after induction were analyzed.Results showed that compared with the control group, the protein carbonyl group increased significantly, and the total sulfhydryl content decreased significantly (P<0.05) with the increase of OA concentration.It was shown that OA could cause the unfolding of SP and exposure of hydrophobic groups with Fourier-transform infrared spectroscopy and surface hydrophobicity.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis results reflected that OA caused the formation of SP polymers.Furthermore, OA could lead to cross-linking of SP disulfide bonds with endogenous fluorescence spectroscopy and surface hydrophobicity analysis.In addition, with the increase of OA concentration, the SP solubility showed a trend of decreasing and increasing turbidity (P<0.05), indicating that OA could trigger changes in the SP structure and the aggregation of SP.

Cite this article

WANG Youjun , ZHOU Limeijia , ZHAO Yangmeijin , AO Chengxiang , LIU Jingyi , ZHAO Hui , LU Hang . Effect of oleic acid on structural and physicochemical properties of sarcoplasmic proteins in Scomberomorus niphonius[J]. Food and Fermentation Industries, 2024 , 50(13) : 239 -246 . DOI: 10.13995/j.cnki.11-1802/ts.036486

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