Food and Fermentation Industries >

2024 , Vol. 50 >Issue 13: 270 - 278

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038603

The mechanism of black mulberry reatment for AD was studied based on HPLC fingerprint and network pharmacology

  • CHEN Xuanshi ,
  • LIU Mengwen ,
  • SHEN Jing ,
  • Ayinuer·Baikere ,
  • HE Shiru ,
  • Tuerxunayi·Dawuti ,
  • XIAO Hui
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  • (School of Public Health, Xinjiang Medical University, Urumqi 830011, China)

Received date: 2024-01-15

  Revised date: 2024-02-26

  Online published: 2024-08-02

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Abstract

The fingerprint of black mulberry was characterized using high-performance liquid chromatography (HPLC) to analyze its primary active constituents.Additionally, the molecular mechanism underlying the treatment of Alzheimer's disease (AD) by black mulberry was investigated through the integration of network pharmacology and molecular docking technology.The fingerprints of 12 batches of black mulberry were established via HPLC, and common peaks were identified.The 12 batches of black mulberry samples were evaluated through similarity evaluation, cluster analysis, principal component analysis, OPLS-DA analysis, and TOPSIS analysis.The active ingredients and target information of black mulberry were obtained from the TCMSP database and the Swiss Target Prediction platform.Disease target information for AD was acquired from the Gene Cards database and AD database to identify potential targets for AD treatment.PPI network diagram was constructed, and topological analysis was performed using the String database and Cytoscape 3.7.2.The core targets were enriched by GO and KEGG using R software.Molecular docking simulations were conducted using Auto Dock software.The HPLC fingerprints of black mulberry samples exhibited a similarity exceeding 0.96, identifying three chromatographic peaks.Cluster analysis categorized the 12 batches of samples into three categories, with principal component analysis results consistent with those of cluster analysis.Twelve core targets were identified after network pharmacological screening, and 1 480 GO entries and 104 KEGG pathways were obtained through enrichment analysis of core targets.Black mulberry may regulate the PI3K-Akt signaling pathway, neutrophil exagination and other signaling pathways through core targets, thereby regulating inflammation and other responses, and achieving the effect of treating and preventing AD through multi-target-multi-pathway.

Key words： black mulberry; Alzheimer's disease; HPLC; fingerprints; network pharmacology

Cite this article

CHEN Xuanshi , LIU Mengwen , SHEN Jing , Ayinuer·Baikere , HE Shiru , Tuerxunayi·Dawuti , XIAO Hui . The mechanism of black mulberry reatment for AD was studied based on HPLC fingerprint and network pharmacology[J]. Food and Fermentation Industries, 2024 , 50(13) : 270 -278 . DOI: 10.13995/j.cnki.11-1802/ts.038603

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