Food and Fermentation Industries >

2024 , Vol. 50 >Issue 14: 97 - 103

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035995

Study on extraction process optimization of anthocyanins from sea red rice and its hypoglycemic effect in vitro

  • ZHONG Kaicui ,
  • LIU Siwei ,
  • WU Yutong ,
  • CHEN Zhongqin ,
  • QIN Xiaoming ,
  • XIE Hangang ,
  • CHEN Mingshen
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  • 1(College of Food Science and Technology, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Ocean University, Zhanjiang 524088, China)
    2(Guangdong Zhenkang Pharmaceutical Technology Co.Ltd., Foshan 528051, China)

Received date: 2023-05-01

  Revised date: 2023-07-21

  Online published: 2024-08-02

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Abstract

The extraction process optimization of anthocyanins from sea red rice and its hypoglycemic effect in vitro were investigated in this study.A series of single-factor experiments and orthogonal experimental designs were used to optimize the extraction process of anthocyanins from sea red rice.The contents of anthocyanins were determined by the pH difference method, and the inhibitory activities of anthocyanins against α-glucosidase and α-amylase were also analyzed.In addition, the enzyme inhibition mode of anthocyanins was studied by the enzyme inhibition kinetics model (α-glucosidase and α-amylase).The optimal extraction conditions of anthocyanins were as follows: pH 0.75, 85% of ethanol, 45 ℃ of extraction temperature, 30 min of extraction time, and the content of anthocyanins was 1.66 mg/mL under these conditions.Anthocyanins of sea red rice had strong inhibition against α-glucosidase (IC50 of 0.11 mg/mL) and α-amylase (IC50 of 0.05 mg/mL).The enzyme inhibition kinetics analysis indicated that anthocyanins of sea red rice showed mixed inhibition mode against two enzymes (α-glucosidase and α-amylase), namely including competitive inhibition and non-competitive inhibition.This study provides a simple method for ultrasonic-assisted extraction of anthocyanins from sea red rice, and the extracted anthocyanins could play a hypoglycemic effect in vitro with mixed inhibition mode.This study would provide a theoretical direction for the development and utilization of sea red rice resources.

Key words： sea red rice; anthocyanins; α-glucosidase; α-amylase; enzyme inhibition kinetics

Cite this article

ZHONG Kaicui , LIU Siwei , WU Yutong , CHEN Zhongqin , QIN Xiaoming , XIE Hangang , CHEN Mingshen . Study on extraction process optimization of anthocyanins from sea red rice and its hypoglycemic effect in vitro[J]. Food and Fermentation Industries, 2024 , 50(14) : 97 -103 . DOI: 10.13995/j.cnki.11-1802/ts.035995

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