Screening of compound fermentation strains for reducing beany flavor of textured soybean protein

  • XU Wen ,
  • ZHANG Xueer ,
  • LIU Zhenyu ,
  • ZHANG Jiaqi ,
  • ZHANG Xiaoqian ,
  • JIA Dongying
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  • 1(College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China)
    2(Mianyang Economic and Technological Development Zone Management-Committee, Mianyang 621052, China)

Received date: 2023-06-28

  Revised date: 2023-08-31

  Online published: 2024-08-02

Abstract

Using headspace solid phase microextraction-gas chromatography-mass spectrometry combined with flavor activity and sensory evaluation, the reduction effect of four strains, which included aroma producing active dry yeast, active dry yeast, Lactobacillus plantarum, and Staphylococcus carnosus, on the beany flavor of textured soybean protein (TSP) were compared.Two strains that could effectively reduce the beany flavor were screened out, and their addition mass ratio and method were studied.Results showed that the four strains could reduce the beany flavor of TSP to a certain degree.The active dry yeast displayed the highest reduction effect, and it decreased the content of beany flavor substances in TSP by 70%.Staphylococcus carnosus had the greatest aroma enhancing effect, and it increased the content of good flavor substances in TSP by 865.41%.Active dry yeast was selected as the deodorant strain, and Staphylococcus carnosus was as the aroma-enhancing strain.The optimal mass ratio of the two strains was 10∶1, and their suitable addition method was fermenting TSP first with Staphylococcus botulinus at 20 ℃ for 12 h and then with active dry yeast at this temperature for 20 h.

Cite this article

XU Wen , ZHANG Xueer , LIU Zhenyu , ZHANG Jiaqi , ZHANG Xiaoqian , JIA Dongying . Screening of compound fermentation strains for reducing beany flavor of textured soybean protein[J]. Food and Fermentation Industries, 2024 , 50(14) : 240 -245 . DOI: 10.13995/j.cnki.11-1802/ts.036604

References

[1] 高育哲, 肖志刚, 刘月菊, 等.大豆组织蛋白的应用和产业现状[J].粮食与油脂, 2020, 33(10):3-4.
GAO Y Z, XIAO Z G, LIU Y J, et al.Application and industry status of textured soybean protein[J].Cereals and Oils, 2020, 33(10):3-4.
[2] ZHANG Y J, FENG Y X, SHI H Q, et al.Impact of steam explosion pretreatment of defatted soybean meal on the flavor of soy sauce[J].LWT, 2022, 156:113034.
[3] YANG A J, SMYTH H, CHALIHA M, et al.Sensory quality of soymilk and tofu from soybeans lacking lipoxygenases[J].Food Science & Nutrition, 2015, 4(2):207-215.
[4] WANG B, ZHANG Q, ZHANG N, et al.Insights into formation, detection and removal of the beany flavor in soybean protein[J].Trends in Food Science & Technology, 2021, 112:336-347.
[5] 李理, 冯静文, 刘力, 等.豆腐酸浆中酵母的分离鉴定及其在酸豆乳中的应用[J].现代食品科技, 2016, 32(5):173-179.
LI L, FENG J W, LIU L, et al.Isolation and identification of yeast from acidic tofu whey and its application to soy yogurt[J].Modern Food Science and Technology, 2016, 32(5):173-179.
[6] 柳旺, 蒋肇样, 贾冬英, 等.米曲霉固态发酵程度对大豆拉丝蛋白特性的影响[J].中国油脂, 2019, 44(5):128-131.
LIU W, JIANG Z Y, JIA D Y, et al.Effects of solid-state fermentation degree by Aspergillus oryzae on the properties of drawing soy protein[J].China Oils and Fats, 2019, 44(5):128-131.
[7] 姚开, 贾冬英, 李湧.通过复合酵母菌发酵降低大豆组织蛋白豆腥味的方法:CN111838404A[P].2020-10-30.
YAO K, JIA D Y, LI Y, et al.A prescription for reducing the beany smell of soybean histone by fermentation with compound yeast:China, CN111838404A[P].2020-10-30.
[8] 李云成, 饶佳薇, 孟凡冰, 等.不同熟制方式对兔肉品质的影响[J].中国调味品, 2022, 47(1):111-118.
LI Y C, RAO J W, MENG F B, et al.Effect of different cooking methods on the quality of rabbit meat[J].China Condiment, 2022, 47(1):111-118.
[9] 白乐宜, 颜振敏, 程娇娇, 等.应用GC-MS和GC-O对比分析中国赊酒与赊店地蕴酒中的香气成分[J].河南科技学院学报(自然科学版), 2018, 46(4):36-39.
BAI L Y, YAN Z M, CHENG J J, et al.Comparative study on aroma components in Chinese credit wine and credit wine by GC-MS and GC-O[J].Journal of Henan Institute of Science and Technology(Natural Science Edition), 2018, 46(4):36-39.
[10] 田甜甜, 王积武, 吴志莲, 等.不同萃取方法对山楂汁香气成分测定的影响[J].食品工业科技, 2014, 35(18):153-156.
TIAN T T, WANG J W, WU Z L, et al.Effect of different extraction method on the identification of aromatic components of hawthorn juice[J].Science and Technology of Food Industry, 2014, 35(18):153-156.
[11] 申光辉, 冯孟, 张志清, 等.生香酵母发酵桑葚低糖复合果酱工艺优化及风味、抗氧化活性变化分析[J].江苏农业学报, 2018, 34(3):669-678.
SHEN G H, FENG M, ZHANG Z Q, et al.Optimization of fermentation conditions and analysis of volatile components and antioxidant activity of low-sugar complex mulberry jam fermented by Wickerhamomyces anomalus Z133[J].Jiangsu Journal of Agricultural Sciences, 2018, 34(3):669-678.
[12] 高天宇. 担子菌门食用型真菌发酵改良大豆膳食纤维风味研究[D].无锡:江南大学, 2021.
GAO T Y.Research on the soybean dietary fiber flavor improvement by fermentation of basidiomycota edible fungi[D].Wuxi:Jiangnan University,2021.
[13] 宗丽娜, 瞿恒贤, 张龙飞, 等.不同混合发酵方式对发酵豆乳特征风味的影响[J].食品与发酵工业, 2023, 49(11):102-110.
ZONG L N, QU H X, ZHANG L F, et al.Effects of different mixed fermentation methods on the characteristic flavor of fermented soymilk[J].Food and Fermentation Industries, 2023, 49(11):102-110.
[14] 韩金志, 潘雯丽, 沈昊, 等.不同乳酸菌发酵豆浆挥发性气味物质的差异性分析[J].中国食品学报, 2021, 21(9):296-305.
HAN J Z, PAN W L, SHEN H, et al.Diversity analysis of volatile odors in soy milk fermented by different lactic acid bacteria[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(9):296-305.
[15] 杨小莹, 肖珊, 庞煜, 等.不同发酵剂对发酵香肠品质的影响[J].食品与发酵工业, 2023,49(18):242-249.
YANG X Y, XIAO S, PANG Y, et al.Different starters on the quality of fermented sausage[J].Food and Fermentation Industries, 2023,49(18):242-249.
[16] VONG W C, LIU S Q.Changes in volatile profile of soybean residue (okara) upon solid-state fermentation by yeasts[J].Journal of the Science of Food and Agriculture, 2017, 97(1):135-143.
[17] 唐育岐, 刘天明, 赵修报, 等.高产2-苯乙醇酵母菌株的筛选及培养基优化[J].中国食品添加剂, 2012(3):137-142.
TANG Y Q, LIU T M, ZHAO X B, et al.Screening of high-yield yeast strains for 2-phenylethanol land its medium optimization[J].China Food Additives, 2012(3):137-142.
[18] 王梦松, 魏超昆, 范敏, 等.GC-MS、DSA结合化学计量学分析稀奶油发酵过程中挥发性风味物质变化[J].食品科学, 2023, 44(16):267-273.
WANG M S, WEI C K, FAN M, et al.Gas chromatography-mass spectrometry and descriptive sensory analysis combined with chemometrics to analyze the changes of volatile flavor substances during the fermentation of cream[J].Food Science, 2023, 44(16):267-273.
[19] 彭雨露. 乳酸菌协同酶解发酵制备稀奶油-大豆分离蛋白复合风味香基的研究[D].无锡:江南大学, 2022.
PENG Y L.Study on cream-soy protein isolate flavors by fermentation in combination with enzymatic hydrolysis[D].Wuxi:Jiangnan University, 2022.
[20] 周慧敏, 张顺亮, 赵冰, 等.木糖葡萄球菌和肉葡萄球菌混合发酵剂对腊肉品质的影响[J].食品科学, 2018, 39(22):32-38.
ZHOU H M, ZHANG S L, ZHAO B, et al.Effect of starter culture mixture of Staphylococcus xylosus and S.carnosus on the quality of dry-cured meat[J].Food Science, 2018, 39(22):32-38.
[21] MIZUNO A,TABEI H,IWAHUTI M.Characterization of low-acetic-acid-producing yeast isolated from 2-deoxyglucose-resistant mutants and its application to high-gravity brewing[J].Journal of Bioscience and Bioengineering, 2006, 101(1):31-37.
[22] VONG W C, LIU S Q.Bioconversion of green volatiles in okara (soybean residue) into esters by coupling enzyme catalysis and yeast (Lindnera saturnus) fermentation[J].Applied Microbiology and Biotechnology, 2018, 102(23):10 017-10 026.
[23] 任丹, 陈曦, 李娟娟, 等.一株水解大豆蛋白细菌的鉴定及其酶学性质研究[J].中国酿造, 2012, 31(12):37-41.
REN D, CHEN X, LI J J, et al.Identification and enzymatic properties of a bacterium strain with hydrolyzing soy protein[J].China Brewing, 2012, 31(12):37-41.
[24] 冯鹏鹏, 孙丽静, 肖冬光, 等.啤酒酵母高级醇的代谢与调控研究进展[J].食品研究与开发, 2021, 42(8):153-159.
FENG P P, SUN L J, XIAO D G, et al.Research progress on metabolism and regulation of higher alcohols in beer yeast[J].Food Research and Development, 2021, 42(8):153-159.
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