Food and Fermentation Industries >

2024 , Vol. 50 >Issue 16: 110 - 117

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037525

Comparison of antioxidant ability of enzymatic hydrolysates from seven conventional freshwater fish and identification of peptide composition

  • DENG Mei ,
  • MEI Qianggen ,
  • XIE Xing ,
  • LU Feiyan ,
  • WANG Shu ,
  • ZHANG Lu ,
  • TU Zongcai
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  • 1(National R&D Center for Freshwater Fish Processing, College of Life Science, Jiangxi Normal University, Nanchang 330022, China)
    2(College of Health, Jiangxi Normal University, Nanchang 330022, China)
    3(State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China)

Received date: 2023-08-29

  Revised date: 2023-10-18

  Online published: 2024-09-19

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Abstract

Freshwater fish is a good source of antioxidant peptides due to its abundant protein and great output.In this study, seven kinds of conventional freshwater fish were used to prepare antioxidant peptides, and the fundamental constituents of raw materials were determined.The peptide fractions with different molecular weights were separated by ultrafiltration technology, the antioxidant activities of each fraction were evaluated by different models in vitro, and the peptide composition was identified by liquid chromatography-tandem mass spectrometry.Results showed that the contents of water, ash, crude protein, and crude fat in seven kinds of freshwater fish were significantly different (P<0.05), and the molecular weight (Mw) of ultrafiltration fractions from enzymatic hydrolysate were mainly distributed in 2 139-2 902 Da and 408-1 156 Da, which accounted for over 90%.The antioxidant activity of peptides with Mw<3 kDa were stronger than that of crude peptides and peptides with Mw>3 kDa.The <3 kDa peptide fractions from grass carp, black carp, and bighead carp showed the strongest ABTS cationic radical scavenging ability [(196.73±16.27) μg/mL], reducing ability [(6.47±0.41) mg/mL], and ferrous ion chelating ability [(114.00±3.41) μg/mL], respectively, which were the best sources of antioxidant peptides.A total of 510 peptide fragments were identified from <3 kDa peptide fractions by mass spectrometry, including KDPIDPWAPGKP, DPIDPPWAPGKP, and VLDRPGPPEGPL.This study could promote the high-value utilization of conventional freshwater fish and guide the development of antioxidant peptides from freshwater fish.

Key words： freshwater fish; antioxidant activity; bioative peptide; structural identification; ultrafiltration

Cite this article

DENG Mei , MEI Qianggen , XIE Xing , LU Feiyan , WANG Shu , ZHANG Lu , TU Zongcai . Comparison of antioxidant ability of enzymatic hydrolysates from seven conventional freshwater fish and identification of peptide composition[J]. Food and Fermentation Industries, 2024 , 50(16) : 110 -117 . DOI: 10.13995/j.cnki.11-1802/ts.037525

References

[1] HU Y M, LU S Z, LI Y S, et al.Protective effect of antioxidant peptides from grass carp scale gelatin on the H2O2-mediated oxidative injured HepG2 cells[J].Food Chemistry, 2022, 373:131539.
[2] RUAN J P, CHEN J D, ZENG J, et al.The protective effects of Nile tilapia (Oreochromis niloticus) scale collagen hydrolysate against oxidative stress induced by tributyltin in HepG2 cells[J].Environmental Science and Pollution Research Interrational, 2019, 26(4):3612-3620.
[3] 朱臻怡, 冯民, 熊华萱, 等.食品中抗氧化剂的应用及其检测技术研究进展[J].化学分析计量, 2013, 22(5):104-108.
ZHU Z Y, FENG M, XIONG H X, et al.Research progress of application and detection technology of antioxidants in food[J].Chemical Analysis and Meterage, 2013, 22(5):104-108.
[4] 付镓榕, 马尚玄, 魏元苗, 等. 澳洲坚果抗氧化肽的分离纯化及肽段鉴定. 食品工业科技, 2024, 45(6):91-99.
FU J R, MA S X, WEI Y M, et al. Separation, purification and identification of antioxidant peptides derived from Macadamia integrifolia. Science and Technology of Food Industry, 2024, 45(6):91-99.
[5] 吉正梅, 张晓春, 彭钰迪, 等.鸭胚源抗氧化肽TD12对HepG2细胞氧化应激损伤的保护作用[J].食品与发酵工业, 2021, 47(18):141-148.
JI Z M, ZHANG X C, PENG Y D, et al.Protective effect of duck embryo-derived antioxidant peptide TD12 on oxidative stress damage in HepG2 cells[J].Food and Fermentation Industries, 2021, 47(18):141-148.
[6] CAI L Y, WU X S, ZHANG Y H, et al.Purification and characterization of three antioxidant peptides from protein hydrolysate of grass carp (Ctenopharyngodon idella) skin[J].Journal of Functional Foods, 2015, 16:234-242.
[7] 农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会编制. 中国渔业统计年鉴-2023. 北京: 中国农业出版社, 2023.
Ministry of Agriculture and Rural Affairs of the People′s Republic of China, National Fisheries Technology Extension Center, Chinese Society of Fisheries. China Fishery Statistical Yearbook-2023. Beijing: China Agriculture Press, 2023.
[8] YATHISHA U G, BHAT I, KARUNASAGAR I, Karunasagark I, et al.Antihypertensive activity of fish protein hydrolysates and its peptides[J].Critical Reviews in Food Science and Nutrition, 2019, 59(15):2363-2374.
[9] 刘丹. 秋刀鱼蛋白抗氧化肽的分离纯化及其抗疲劳功效研究[D].广州:华南理工大学, 2015.
LIU D. Study on the purification of antioxidant peptide from Pacific saury protein and its antifatigue activities. Guangzhou: South China University of Technology, 2015.
[10] HASHEM A M A, VENMARATH A, KUDRE T G.Preparation, purification, and identification of novel antioxidant peptides from red-bellied pacu (Piaractus brachypomus) fish meat protein hydrolysate[J].Food Science and Biotechnology, 2023, 32(14):2057-2068.
[11] 朱敏方. 草鱼鱼肉抗氧化肽的制备、分离鉴定及其活性研究[D].南昌:江西师范大学, 2020.
ZHU M F.Preparation, isolation, identification and activity of antioxidant peptides from grass carp fish (Ctenopharyngodon Idella) muscle[D].Nanchang:Jiangxi Normal University, 2020.
[12] 李军, 罗娟, 涂宗财, 等.鲢鱼骨胶原多肽的制备及其抗氧化活性研究[J].食品与发酵工业, 2020, 46(2):222-230.
LI J, LUO J, TU Z C, et al.Study on the preparation and antioxidant activity of collagen polypeptide from silver carp bone[J].Food and Fermentation Industries, 2020, 46(2):222-230.
[13] 贾韶千, 李艳霞.黄鳝鱼骨多肽制备及其抗氧化活性[J].食品科学, 2016,37(1):133-138.
JIA S Q, LI Y X.Preparation and antioxidant activity of Monopterus albus bone peptides[J].Food Science, 2016, 37(1):133-138.
[14] 张美玲, 赵新淮.大豆蛋白水解物的酶法修饰及其亚铁和钙离子的螯合能力[J].食品与发酵工业, 2012, 38(12):26-30.
ZHANG M L, ZHAO X H.Enzymatic modification of soybean protein hydrolysates by plastein reaction and its influence on chelating activities for ferrous and calcium ions[J].Food and Fermentation Industries, 2012, 38(12):26-30.
[15] 黄湘芬, 陈开健, 许宝红, 等.不同饲养条件下的鳙鱼肌肉营养成分研究[J].当代水产, 2011, 36(7):57-61.
HUANG X F, CHEN K J, XU B H, et al.Study on nutritional components of bighead carp muscle under different feeding conditions[J].Current Fisheries, 2011, 36(7):57-61.
[16] 魏永生, 李维维, 隆晶, 等.GC/MS测定鲢鱼肌肉中的脂肪酸组成[J].广州化工, 2017, 45(5):74-76.
WEI Y S, LI W W, LONG J, et al.Determination of fatty acid compositions in muscle of silver carp by GC/MS[J].Guangzhou Chemical Industry, 2017, 45(5):74-76.
[17] 李亚会, 李积华, 吉宏武, 等.远东拟沙丁鱼抗氧化肽的分离纯化及结构解析[J].中国食品学报, 2021, 21(2):229-238.
LI Y H, LI J H, JI H W, et al.Isolation, purification and structural analysis of antioxidant peptides from Sardinops sagax[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(2):229-238.
[18] 左依瑾, 于子淇, 申雪晴, 等.鱼源抗氧化肽的结构与其功能关系研究进展[J].食品工业科技, 2023, 44(14):419-429.
ZUO Y J, YU Z Q, SHEN X Q, et al.Recent advance on the structure-activity relationship in antioxidant peptides of fish[J].Science and Technology of Food Industry, 2023, 44(14):419-429.
[19] 冯晓文, 赵晓涵, 潘骁琦, 等.体外模拟消化对海洋鱼骨胶原低聚肽结构和抗氧化活性的影响[J].食品与发酵工业, 2022, 48(5):173-179.
FENG X W, ZHAO X H, PAN X Q, et al.The effect of simulated digestion in vitro on structure and antioxidant activity of marine fish bone collagen oligopeptides[J].Food and Fermentation Industries, 2022, 48(5):173-179.
[20] 乔虹, 黎松松, 周南希, 等. 海洋来源金属离子螯合肽研究进展. 食品工业科技, 2024, 45(1):368-377.
QIAO H, LI S S, ZHOU N X, et al. Research progress in metal ion chelated peptides of marine sources. Science and Technology of Food Industry, 2024, 45(1):368-377.
[21] JIA X Y, ZHU M F, ZHANG L, et al.Extraction optimization and screening of antioxidant peptides from grass carp meat and synergistic-antagonistic effect[J].Food Science & Nutrition, 2022, 10(5):1481-1493.
[22] 范三红, 贾槐旺, 李兰, 等.紫苏籽粕蛋白源抗氧化肽的纯化、结构鉴定及体外抗氧化活性[J].中国粮油学报, 2022, 37(3):79-87.
FAN S H, JIA H W, LI L, et al.Purification and structural identification of antioxidant peptide from perilla seed meal protein and antioxidant activity in vitro[J].Journal of the Chinese Cereals and Oils Association, 2022, 37(3):79-87.
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