Lipase-catalyzed grafting of gallic acid onto pectin molecules was investigated in various organic solvent/water systems, including DMSO/water, DMF/water, THF/water, and MTBE/water.The study aimed to evaluate the effect of these solvents on the grafting reaction and the resulting impact on the antioxidant and antibacterial properties of the modified pectin.The chemical structures of the synthesized gallic acid-pectin copolymers were characterized using ultraviolet-visible spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy techniques.The grafting ratio of gallic acid onto pectin varied significantly depending on the polarity of the solvent system employed.The highest grafting ratio of 20.36% was achieved using the MTBE/water system, resulting in the formation of MTBE-Pe copolymer.The incorporation of gallic acid onto the pectin backbone significantly enhanced its antioxidant activity and antibacterial efficacy against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria.A positive correlation was observed between the grafting ratio and the antioxidant and antibacterial activities of the modified pectin.The lipase-catalyzed grafting approach presented in this study offered a novel method for the preparation of pectin derivatives with enhanced antioxidant and antibacterial properties.These modified pectin copolymers showed promise as natural preservatives for potential applications in the food and cosmetic industries, where the demand for safe and effective natural additives was increasing.
ZHANG Zehong
,
GUO Sutong
,
LI Yiya
,
FEI Peng
. Enzymatic synthesis of gallic acid-pectin conjugates: Solvent effects and functional properties[J]. Food and Fermentation Industries, 2024
, 50(17)
: 156
-161
.
DOI: 10.13995/j.cnki.11-1802/ts.039372
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