Research progress on the metabolism of γ-aminobutyric acid and the mechanism of stress accumulation in germinated grains

  • LU Jing ,
  • BAI Shuqun ,
  • ZHENG Xueling
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  • 1(College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China)
    2(Beijing Key Laboratory of Nutrition, Health & Food Safety, Nutrition & Health Research Institute, COFCO Corporation, Beijing 102200, China)

Received date: 2023-11-13

  Revised date: 2023-12-08

  Online published: 2024-10-10

Abstract

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the human central nervous system.GABA has many effects such as relieving anxiety, insomnia, and anti-aging, etc.Therefore, appropriately intaking of GABA from diet is essential for human health, and grains are one of those food sources.Germinated grains are green, flavorful, natural health foods and they are rich in GABA.During the process of grain germinating, GABA is metabolized through the GABA shunt and polyamine metabolic pathway.This paper described these metabolic pathways of GABA synthesis.Enzyme activities, gene expression levels and external factors have different impacts on the accumulation of GABA.GABA is rapidly accumulated during grain responses to abiotic stress.The different factors which affecting the GABA accumulation metabolism were reviewed.We summarized the recent research on GABA accumulation and mechanism with the goal of providing a review of GABA in grains.

Cite this article

LU Jing , BAI Shuqun , ZHENG Xueling . Research progress on the metabolism of γ-aminobutyric acid and the mechanism of stress accumulation in germinated grains[J]. Food and Fermentation Industries, 2024 , 50(17) : 380 -386 . DOI: 10.13995/j.cnki.11-1802/ts.037937

References

[1] 张伊迪. 青稞发芽过程中营养成分含量变化的研究[D].上海:上海交通大学, 2016.
ZHANG Y D.Exploration of content change of several nutritional ingredients in germination process of highland barley[D].Shanghai:Shanghai Jiao Tong University, 2016.
[2] BOLNEO E, CHAU P Y S, NOAKES P G, et al.Investigating the role of GABA in neural development and disease using mice lacking GAD67 or VGAT genes[J].International Journal of Molecular Sciences, 2022, 23(14):7965.
[3] GEISLER C E, GHIMIRE S, BRUGGINK S M, et al.A critical role of hepatic GABA in the metabolic dysfunction and hyperphagia of obesity[J].Cell Reports, 2021, 35(13):109301.
[4] ZHAO N, SHU Y, JIAN C X, et al.Lactobacillus ameliorates SD-induced stress responses and gut dysbiosis by increasing the absorption of gut-derived GABA in rhesus monkeys[J].Frontiers in Immunology, 2022, 13:915393.
[5] 何维, 宋贺, 扬子彪, 等.外源γ-氨基丁酸通过调节肠道菌群改善神经系统疾病研究进展[J].食品工业科技, 2023, 44(6):432-440.
HE W, SONG H, YANG Z B, et al.Research progress of exogenous γ-aminobutyric acid in improving neurological diseases by regulating intestinal flora[J].Science and Technology of Food Industry, 2023, 44(6):432-440.
[6] 姜秀杰, 许庆鹏, 张爱武, 等.植物代谢法富集粮食中γ-氨基丁酸的研究进展[J].黑龙江八一农垦大学学报, 2021, 33(1):33-39.
JIANG X J, XU Q P, ZHANG A W, et al.Research progress on γ-aminobutyric acid accumulation in grain by plant metabolism method[J].Journal of Heilongjiang Bayi Agricultural University, 2021, 33(1):33-39.
[7] ISLAM M Z, AN H G, KANG S J, et al.Physicochemical and bioactive properties of a high β-glucan barley variety ‘Betaone’ affected by germination processing[J].International Journal of Biological Macromolecules, 2021, 177:129-134.
[8] RAMOS-RUIZ R, MARTINEZ F, KNAUF-BEITER G.The effects of GABA in plants[J].Cogent Food & Agriculture, 2019, 5(1):1670553.
[9] SHELP B J, BOZZO G G, TROBACHER C P, et al.Hypothesis/review:Contribution of putrescine to γ-aminobutyrate (GABA) production in response to abiotic stress[J].Plant Science:an International Journal of Experimental Plant Biology, 2012, 193-194:130-135.
[10] BAGNI N, TASSONI A.Biosynthesis, oxidation and conjugation of aliphatic polyamines in higher plants[J].Amino Acids, 2001, 20(3):301-317.
[11] PÁL M, SZALAI G, JANDA T.Speculation:Polyamines are important in abiotic stress signaling[J].Plant Science:an International Journal of Experimental Plant Biology, 2015, 237:16-23.
[12] 孙磊, 王苑, 柏映国, 等.谷氨酸脱羧酶结构及催化机制的研究概述[J].微生物学通报, 2020, 47(7):2236-2244.
SUN L, WANG Y, BAI Y G, et al.Structure and catalytic mechanism of glutamate decarboxylase:A review[J].Microbiology China, 2020, 47(7):2236-2244.
[13] KHWANCHAI P, CHINPRAHAST N, PICHYANGKURA R, et al.Gamma-aminobutyric acid and glutamic acid contents, and the GAD activity in germinated brown rice (Oryza sativa L.):Effect of rice cultivars[J].Food Science and Biotechnology, 2014, 23(2):373-379.
[14] ELBALOULA M F, HASSAN A B.Effect of different salt concentrations on the gamma-aminobutyric-acid content and glutamate decarboxylase activity in germinated sorghum (Sorghum bicolor L.Moench) grain[J].Food Science & Nutrition, 2022, 10(6):2050-2056.
[15] 周露. 富含γ-氨基丁酸(GABA)转基因水稻的研究[D].南京:南京农业大学, 2015.
ZHOU L.Study on transgenic rice rich in γ-aminobutyric acid (GABA)[D].Nanjing:Nanjing Agricultural University, 2015.
[16] 刘玲珑. 稻米浸水后γ-氨基丁酸(GABA)的积累及其分子机制研究[D].南京:南京农业大学, 2005.
LIU L L.Accumulation of γ-aminobutyric acid (GABA) in rice after soaking and its molecular mechanism[D].Nanjing:Nanjing Agricultural University, 2005.
[17] 刘丽妹. 发芽糙米γ-氨基丁酸富集及关键酶基因的表达分析[D].厦门:集美大学, 2015.
LIU L M.Accumulation of γ-aminobutyric acid in germinated brown rice and key enzyme gene expression analysis[D].Xiamen:Jimei University, 2015.
[18] ZHAO G C, XIE M X, WANG Y C, et al.Molecular mechanisms underlying γ-aminobutyric acid (GABA) accumulation in giant embryo rice seeds[J].Journal of Agricultural and Food Chemistry, 2017, 65(24):4883-4889.
[19] WATTERSON D M, IVERSON D B, VAN ELDIK L J.Spinach calmodulin:Isolation, characterization, and comparison with vertebrate calmodulins[J].Biochemistry, 1980, 19(25):5762-5768.
[20] LING V, SNEDDEN W A, SHELP B J, et al.Analysis of a soluble calmodulin binding protein from fava bean roots:Identification of glutamate decarboxylase as a calmodulin-activated enzyme[J].The Plant Cell, 1994, 6(8):1135-1143.
[21] 尹永祺. NaCl及其联合Ca~(2+)处理下发芽大豆生理变化与GABA富集调控机理[D].南京:南京农业大学, 2014.
YIN Y Q.Study on the physiological change and the regulation mechanism of GABA accumulation in germinating soybean under NaCl and NaCl-Ca2+ treatment[D].Nanjing:Nanjing Agricultural University, 2014.
[22] 王宪青, 魏彤, 石彦国.绿豆钙调素的分离纯化及其对谷氨酸脱羧酶的调节作用[J].粮食与油脂, 2020, 33(9):39-43.
WANG X Q, WEI T, SHI Y G.Extraction and purification of mung bean calmodulin and its regulation on glutamate decarboxylase[J].Cereals & Oils, 2020, 33(9):39-43.
[23] ARAZI T, BAUM G, SNEDDEN W A, et al.Molecular and biochemical analysis of calmodulin interactions with the calmodulin-binding domain of plant glutamate decarboxylase[J].Plant Physiology, 1995, 108(2):551-561.
[24] 鲍佐宝. 发芽苦荞GABA-T的酶学特性、基因克隆及序列分析[D].滁州:安徽科技学院, 2019.
BAO Z B.Study on enzymatic characteristics, gene cloning and sequence analysis of GABA-T from germinated tartary buckwheat[D].Chuzhou:Anhui Science and Technology University, 2019.
[25] 孙莹. 藜麦再生体系的构建与γ-氨基丁酸转氨酶(GABA-T)多态性分析及含量测定[D].济南:山东师范大学, 2019.
SUN Y.Construction of quinoa regeneration system with γ-aminobutyric acid transaminase (GABA-T) polymorphism analysis and determination of its content[D].Jinan:Shandong Normal University, 2019.
[26] 周露, 沈贝贝, 白苏阳, 等.以RNA干扰γ-氨基丁酸转氨酶1基因(OsGABA-T1)表达提高稻米γ-氨基丁酸(GABA)含量[J].作物学报, 2015, 41(9):1305-1312.
ZHOU L, SHEN B B, BAI S Y, et al.RNA interference of OsGABA-T1Gene expression induced GABA accumula-tion in rice grain[J].Acta Agronomica Sinica, 2015, 41(9):1305-1312.
[27] 苏国兴. 多胺分解代谢在大豆生长发育和耐盐生理中的作用[D].南京:南京农业大学, 2006.
SU G X.The roles of polyamine catabolism in development and salt tolerance of soybean seedlings[D].Nanjing:Nanjing Agricultural University, 2006.
[28] 张瀚竹. 大豆多胺氧化酶基因GmPAO1的克隆及耐盐功能分析[D].长春:吉林农业大学, 2022.
ZHANG H Z.Cloning and salt tolerance analysis of soybean polyamine oxidase gene GmPAO1[D].Changchun:Jilin Agricultural University, 2022.
[29] LI L, DOU N, ZHANG H, et al.The versatile GABA in plants[J].Plant Signaling & Behavior, 2021, 16(3):1862565.
[30] 李婷玉, 杜艳, 陈正行, 等.胁迫萌发对青稞籽粒中β-葡聚糖和γ-氨基丁酸含量的影响[J].中国粮油学报, 2021, 36(6):30-35.
LI T Y, DU Y, CHEN Z X, et al.Effects of highland barley seed germination under stress on contents of β-glucan and γ-aminobutyric acid[J].Journal of the Chinese Cereals and Oils Association, 2021, 36(6):30-35.
[31] WANG M, ZHU Y H, WANG P, et al.Effect of γ-aminobutyric acid on phenolics metabolism in barley seedlings under low NaCl treatment[J].Antioxidants, 2021, 10(9):1421.
[32] 陈春旭, 王利勤, 郭元新, 等.盐胁迫对发芽糙米富集γ-氨基丁酸及蛋白组分变化的影响[J].食品科学, 2018, 39(5):87-92.
CHEN C X, WANG L Q, GUO Y X, et al.Effect of salt stress on γ-aminobutyric acid accumulation and protein composition in germinated brown rice[J].Food Science, 2018, 39(5):87-92.
[33] XING S G, JUN Y B, HAU Z W, et al.Higher accumulation of gamma-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max (L.) Merr.roots[J].Plant Physiology and Biochemistry:PPB, 2007, 45(8):560-566.
[34] 齐菲. 外源Spd调控NaCl胁迫下发芽大豆生理代谢及GABA富集研究[D].扬州:扬州大学, 2019.
QI F.Exogenous Spd regulates physiological metabolism and GABA accumulation research of germinated soybean under NaCl stress[D].Yangzhou:Yangzhou University, 2019.
[35] GÓMEZ-ÁLVAREZ E M, PUCCIARIELLO C.Cereal germination under low oxygen:Molecular processes[J].Plants, 2022, 11(3):460.
[36] DU Y, CHEN Z X, LIANG F, et al.Effects of hypoxia stress germination on nutrients, physicochemical properties and cooking characteristics of highland barley[J].Journal of Cereal Science, 2022, 103:103411.
[37] 陈晓嫚. 低温联合低氧胁迫下发芽大麦γ-氨基丁酸富集技术及产品开发研究[D].滁州:安徽科技学院, 2017.
CHEN X M.Accumulation mechanism of γ-aminobutyric acid under the condition of low temperature joint and hypoxia stress in germination barley and product development[D].Chuzhou:Anhui Science and Technology University, 2017.
[38] WANG P, LIU K X, GU Z X, et al.Enhanced γ-aminobutyric acid accumulation, alleviated componential deterioration and technofunctionality loss of germinated wheat by hypoxia stress[J].Food Chemistry, 2018, 269:473-479.
[39] 王凯凯, 孙朦, 宋佳敏, 等.γ-氨基丁酸(GABA)形成机理及富集方法的研究进展[J].食品工业科技, 2018, 39(14):323-329.
WANG K K, SUN M, SONG J M, et al.Research progress in the formation mechanism and accumulation methods of γ-aminobutyric acid(GABA)[J].Science and Technology of Food Industry, 2018, 39(14):323-329.
[40] 李婷玉. 萌发青稞降糖、降脂、降压活性研究及其理化性质研究[D].无锡:江南大学, 2021.
LI T Y.Study on hypoglycemic, hypolipidemic, hypotensive Activities and physicochemical property of germinated highland barley[D].Wuxi:Jiangnan University, 2021.
[41] 彭红满. 胁迫法富集小米糙米中γ-氨基丁酸的研究[D].石家庄:河北科技大学, 2016.
PENG H M.Accumulation of γ-aminobutyric acid in millet brown rice in response to soak stress and cold shock stress[D].Shijiazhuang:Hebei University of Science & Technology, 2016.
[42] YANG R Q, FENG L, WANG S F, et al.Accumulation of γ-aminobutyric acid in soybean by hypoxia germination and freeze-thawing incubation[J].Journal of the Science of Food and Agriculture, 2016, 96(6):2090-2096.
[43] YANG R Q, HUI Q R, FENG X Y, et al.The mechanism of freeze-thawing induced accumulation of γ-aminobutyric acid in germinated soybean[J].Journal of the Science of Food and Agriculture, 2020, 100(3):1099-1105.
[44] MAZZUCOTELLI E, TARTARI A, CATTIVELLI L, et al.Metabolism of gamma-aminobutyric acid during cold acclimation and freezing and its relationship to frost tolerance in barley and wheat[J].Journal of Experimental Botany, 2006, 57(14):3755-3766.
[45] DING J Z, HOU G G, DONG M Y, et al.Physicochemical properties of germinated dehulled rice flour and energy requirement in germination as affected by ultrasound treatment[J].Ultrasonics Sonochemistry, 2018, 41:484-491.
[46] 张祎, 赵婷婷, 申娟利, 等.超声波处理对发芽糙米GABA积累及抗氧化能力影响的研究[J].食品工业科技, 2016, 37(2):130-133;137.
ZHANG Y, ZHAO T T, SHEN J L, et al.Study on effect of ultrasonic treatment on GABA accumulation and antioxidant capacity in germinated brown rice[J].Science and Technology of Food Industry, 2016, 37(2):130-133;137.
[47] DING J Z, ULANOV A V, DONG M Y, et al.Enhancement of gama-aminobutyric acid (GABA) and other health-related metabolites in germinated red rice (Oryza sativa L.) by ultrasonication[J].Ultrasonics Sonochemistry, 2018, 40(Pt A):791-797.
[48] DING J Z, HOU G G, NEMZER B V, et al.Effects of controlled germination on selected physicochemical and functional properties of whole-wheat flour and enhanced γ-aminobutyric acid accumulation by ultrasonication[J].Food Chemistry, 2018, 243:214-221.
[49] YANG H, GAO J Y, YANG A S, et al.The ultrasound-treated soybean seeds improve edibility and nutritional quality of soybean sprouts[J].Food Research International, 2015, 77:704-710.
[50] CHEN G Y, WANG Y S, ZHANG M Y, et al.Cold atmospheric plasma treatment improves the γ-aminobutyric acid content of buckwheat seeds providing a new anti-hypertensive functional ingredient[J].Food Chemistry, 2022, 388:133064.
[51] HIJAZ F, KILLINY N.Exogenous GABA is quickly metabolized to succinic acid and fed into the plant TCA cycle[J].Plant Signaling & Behavior, 2019, 14(3):e1573096.
[52] MA Y, WANG P, GU Z X, et al.Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress[J].Food Chemistry:X, 2019, 2:100023.
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