Food and Fermentation Industries >

2024 , Vol. 50 >Issue 18: 115 - 122

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037176

Optimization of carboxymethylation modification process and study on biological activity of Laminaria japonica polysaccharide

  • YANG Jiaxin ,
  • LI Yao ,
  • HUANG Xianjian ,
  • XIAO Shixiu ,
  • FENG Shuzhen
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  • 1(College of Medicine, Guangxi University of Science and Technology, Liuzhou 545006, China)
    2(College of Science, Guangxi University of Science and Technology, Liuzhou 545006, China)

Received date: 2023-08-28

  Revised date: 2023-10-18

  Online published: 2024-10-14

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Abstract

This study aimed to optimize the carboxymethylation modification process of Laminaria japonica polysaccharide and study the structure and biological activity of Laminaria japonica polysaccharide before and after modification.The process of carboxymethylation modification of Laminaria japonica polysaccharide was optimized by a single-factor test and an orthogonal test.The structure of Laminaria japonica polysaccharide before and after modification was characterized by ultraviolet spectroscopy, Fourier transform infrared spectroscopy, periodate oxidation, and Congo red experiment.The differences in free radical scavenging ability and hypoglycemic activity of Laminaria japonica polysaccharide before and after modification were compared.Results showed that the optimum conditions were that the alkalization temperature was 50 ℃, the reaction time was 4.0 h, the reaction temperature was 65 ℃, the mass ratio of polysaccharide to chloroacetic acid was 3∶7, and the highest substitution degree was 0.304.Infrared spectroscopy indicated that the carboxymethylation modification was successful, and the triple helix structure disappeared after modification.The carboxymethylated polysaccharide was a pyranoid polysaccharide, and the glucosidic linkages were mainly (1→3) and (1→2,1→4), and the molar ratio was 0.977∶0.023.Carboxymethylation modification significantly improved the scavenging ability of Laminaria japonica polysaccharides on DPPH free radicals, ·OH, and ·O-2, and the scavenging rates increased by 7.38%, 12.17%, and 0.78%, respectively (P<0.05), but there was no significant difference in hypoglycemic activity.These results indicate that the application of carboxymethylation modification to promote the biological activity of Laminaria japonica polysaccharide can give priority to its free radical scavenging ability.

Key words： Laminaria japonica polysaccharide; carboxymethylation; substitution degree; free radical scavenging ability; hypoglycemic activity

Cite this article

YANG Jiaxin , LI Yao , HUANG Xianjian , XIAO Shixiu , FENG Shuzhen . Optimization of carboxymethylation modification process and study on biological activity of Laminaria japonica polysaccharide[J]. Food and Fermentation Industries, 2024 , 50(18) : 115 -122 . DOI: 10.13995/j.cnki.11-1802/ts.037176

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