Food and Fermentation Industries >

2024 , Vol. 50 >Issue 18: 194 - 200;208

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037229

Antioxidant and probiotic growth activities of polysaccharide extracted from Passiflora edulis peel by using pectinase

  • WANG Xiuli ,
  • XUE Dengping ,
  • YI Liang ,
  • LI Lifen ,
  • LI Jing ,
  • LI Xia
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  • (College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, China)

Received date: 2023-09-01

  Revised date: 2023-10-16

  Online published: 2024-10-14

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Abstract

In this paper, Passiflora edulis peel was used as a raw material, and the polysaccharide of the fruit peel was extracted by using an enzymatic method.Its antioxidant and probiotic growth activities were also studied.The extraction parameters of P.edulis peel polysaccharide were optimized based on the orthogonal experiments.Its antioxidant activities were investigated by measuring its reducing ability and scavenging rates of DPPH free radicals and hydroxyl radicals.P.edulis peel polysaccharide was used as the sole carbon source to study its in vitro proliferative effects on Lactobacillus plantarum, L.delbrueckii (subsp.) bulgaricus, L.brevis, and Streptococcus thermophilus. Results showed that the extraction conditions were as follows:pectinase concentration of 5 mg/mL, pH 4.5, 2 h extraction time, and 40 ℃ extraction temperature.The polysaccharide yield of the extraction using these parameters was 15.83%.The P.edulis peel polysaccharide had moderate levels of reducing ability and DPPHs radical scavenging rate, which showed dose-dependent relationships.The polysaccharide sample enhanced the growth of these probiotics.Its maximum growth rates were as follows:L.plantarum (0.159 9)>S.thermophilus (0.139 5)>L.brevis (0.125 1)>L.delbrueckii (subsp.) bulgaricus (0.123 9).Therefore, P.edulis peel polysaccharide is a natural food-based antioxidant and prebiotic substance with good market prospects.

Key words： passion fruit polysaccharides; pectinase; orthogonal test; antioxidant activity; probiotic growth

Cite this article

WANG Xiuli , XUE Dengping , YI Liang , LI Lifen , LI Jing , LI Xia . Antioxidant and probiotic growth activities of polysaccharide extracted from Passiflora edulis peel by using pectinase[J]. Food and Fermentation Industries, 2024 , 50(18) : 194 -200;208 . DOI: 10.13995/j.cnki.11-1802/ts.037229

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