Food and Fermentation Industries >

2024 , Vol. 50 >Issue 19: 81 - 86

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037871

Isolation and identification of strains of Platycodon grandiflorum (PG) solution processed with rice swill and changes of platycodon saponin D content of PG fermented by pure strains

  • CHEN Liyan ,
  • JIA Zhichao ,
  • YU Xinxin ,
  • DING Chunjie ,
  • SUN Yinling ,
  • ZHENG Hongyu ,
  • ZHAO Han ,
  • WANG Weiming
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  • (Heilongjiang Academy of Traditional Chinese Medicine, Harbin 150036, China)

Received date: 2023-11-06

  Revised date: 2023-12-13

  Online published: 2024-10-29

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Abstract

The research aimed to identify the dominant bacteria in the preparation liquid of Platycodon grandiflorum (PG) processed with rice swill and explore the impact of PG fermentation by these dominant bacteria on platycodon saponin D content.PG underwent processing with rice swill, and the dominant bacteria in the preparation liquid were isolated and purified using MRS medium.Identification of the isolated strains involved Gram staining, catalase testing, and 16S rDNA sequencing.PG underwent fermentation with these three purified strains, and the platycodon saponin D content was determined using HPLC.Three strains of lactic acid bacteria, namely Weissella cibaria, Enterococcus faecalis, and Lactobacillus casei, were isolated.It was found that the platycodon saponin D content in fermented PG was higher than that in unfermented PG.Notably, the first two strains, Weissella cibaria and Enterococcus faecalis, increased the platycodon saponin D content in the fermented PG extract by 8.19% and 10.72%, respectively.Lactic acid bacteria emerged as the dominant bacterial group in the rice swill-processed PG preparation liquid.Pure strain fermentation of PG led to an increase in the platycodon saponin D content, suggesting microbial transformation during the rice swill processing of PG, thereby enhancing its activity.

Key words： Platycodon grandiflorum; rice swill; processing; dominant bacteria; pure fermentation; platycodon saponin D

Cite this article

CHEN Liyan , JIA Zhichao , YU Xinxin , DING Chunjie , SUN Yinling , ZHENG Hongyu , ZHAO Han , WANG Weiming . Isolation and identification of strains of Platycodon grandiflorum (PG) solution processed with rice swill and changes of platycodon saponin D content of PG fermented by pure strains[J]. Food and Fermentation Industries, 2024 , 50(19) : 81 -86 . DOI: 10.13995/j.cnki.11-1802/ts.037871

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