Food and Fermentation Industries >

2024 , Vol. 50 >Issue 19: 191 - 200

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039997

Antioxidant and hypoglycemic activities of purple Prunus cerasifera Ehrh extracts and identification of chemical constituents

  • LUO Shibo ,
  • LIU Wei ,
  • XIE Xing ,
  • FENG Yaping ,
  • PENG Chunyan ,
  • ZHANG Lu
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  • 1(Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources, School of Chemistry and Chemical Engineering, Yili Normal University, Yining 835000, China)
    2(College of Health, Jiangxi Normal University, Nanchang 330022, China)
    3(National R&D center of Freshwater Fish Processing, College of Life Science, Jiangxi Normal University, Nanchang 330022, China)

Received date: 2024-05-25

  Revised date: 2024-07-05

  Online published: 2024-10-29

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Abstract

Prunus cerasifera Ehrh is rich in polyphenols and named ‘precious fruit in the snow region', but the anti-diabetic activity and its bioactive compounds remain unclear.In this study, 70% ethanol extraction and solvent fraction were applied to extract the polyphenols in purple PE (PPE).The antioxidant and hypoglycemic activities were evaluated by radical scavenging ability, enzymes inhibition ability, promotion of glucose consumption in HepG2 cells and anti-glycation ability assays.The major bioactive compounds were identified by liquid chromatography-mass spectroscopy technique.Results indicated that the ethyl acetate fraction of PPE showed the highest total phenolics (219.27 mg GAE/g E) and total flavonoids (165.58 mg QuE/g E) content, along with the strongest ABTS cation radical scavenging ability (163.36 μg /mL), α-glucosidase inhibition activity (28.95 μg /mL), promotion glucose absorption in HepG2 cells and anti-glycation abilities.The α-glucosidase inhibition was 31 times higher than that of acarbose.The n-butanol fraction of PPE showed the highest DPPH radical scavenging and DPP-4 enzyme inhibitory capacity.Forty-three compounds were identified from EAF by HPLC-QTOF-MS/MS, including 12 organic acids, 9 phenolic acids, 16 flavonoids and 6 other class of compounds.Therefore, ethyl acetate and n-butanol are the suitable solvents to extract active ingredients from PPE, phenolic acids and flavonoids are the major antioxidants and hypoglycemic compounds.This study could provide theoretical guidance for the application of PPE in hypoglycemic nutritional foods or dietary supplements.

Key words： Prunus cerasifera Ehrh; phenolics; antioxidant activity; hypoglycemic activity; identification

Cite this article

LUO Shibo , LIU Wei , XIE Xing , FENG Yaping , PENG Chunyan , ZHANG Lu . Antioxidant and hypoglycemic activities of purple Prunus cerasifera Ehrh extracts and identification of chemical constituents[J]. Food and Fermentation Industries, 2024 , 50(19) : 191 -200 . DOI: 10.13995/j.cnki.11-1802/ts.039997

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