Food and Fermentation Industries >

2024 , Vol. 50 >Issue 19: 201 - 208

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040140

Components analysis and lowering uric acid ability of anti-xanthine oxidase active extract from Gynura procumbens (Lour.) Merr.

  • FU Rongbo ,
  • LIU Yue ,
  • CHEN Mengzhen ,
  • ZHONG Yuan ,
  • ZHOU Li ,
  • YU Lide
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  • 1(School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China)
    2(Innovation and Entrepreneurship School, Jiangxi University of Chinese Medicine, Nanchang 330004, China)

Received date: 2024-06-08

  Revised date: 2024-07-03

  Online published: 2024-10-29

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Abstract

Inhibiting the activity of xanthine oxidase (XOD) to reduce body uric acid production is an effective way to prevent or treat hyperuricemia.To obtain highly safe XOD activity inhibitors from natural sources, the ultrasonic extraction conditions were optimized using XOD activity inhibition rate as the indicator to get the XOD activity inhibition extract (GE) from an edible and medicinal resource, Gynura procumbens (Lour.) Merr..The composition of GE was identified with liquid chromatography-mass spectrometry technology, and its effect on reducing blood uric acid in hyperuricemia mice was tested.Through ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry (UPLC-Q/TOF-MS) analysis, it was found that GE mainly contains chlorogenic acids, including 4 mono-caffeoyl quinic acids and 6 di-caffeoyl quinic acids, as well as 6 flavonoids, including quercetin and its three glycosides, kaempferol and one glycoside.The XOD activity inhibition rates of ethyl acetate extraction fraction (GEA) and n-butanol extraction fraction (GEB) derived from GE were higher than that of GE.Animal experiment results showed that GE, GEA, and GEB effectively reduced serum uric acid levels and liver XOD activity induced by hypoxanthine and potassium oxazinate (P<0.01), significantly lowered liver malondialdehyde levels (P<0.01), increased antioxidant enzyme superoxide dismutase and catalase activities, reduced the levels of serum cytokine IL-6 and TNF-α (P<0.05), restored IL-10 level (P<0.01), thereby alleviating renal urate deposition and inflammation, and GEA and GEB had better effects than GE.The research results can provide a theoretical basis for the development of uric acid lowering and liver and kidney protection products from food and drug resources such as Gynura procumbens (Lour.) Merr..

Key words： Gynura procumbens (Lour.) Merr.; xanthine oxidase inhibition rate; liquid chromatography-mass spectrometry; hyperuricemia; anti-oxidation; urate deposition; inflammation

Cite this article

FU Rongbo , LIU Yue , CHEN Mengzhen , ZHONG Yuan , ZHOU Li , YU Lide . Components analysis and lowering uric acid ability of anti-xanthine oxidase active extract from Gynura procumbens (Lour.) Merr.[J]. Food and Fermentation Industries, 2024 , 50(19) : 201 -208 . DOI: 10.13995/j.cnki.11-1802/ts.040140

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