Food and Fermentation Industries >

2024 , Vol. 50 >Issue 20: 79 - 87

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037719

Effect of ultrasound treatment on structural and functional properties of Xanthoceras sorbifolium Bunge seeds meal protein

  • LU Qiuling ,
  • LI Lang ,
  • YANG Min ,
  • WEI Yanming
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  • (College of Science, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2023-10-22

  Revised date: 2023-12-22

  Online published: 2024-11-01

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Abstract

Xanthoceras sorbifolium Bunge seeds meal is the main by-product in the production of Xanthoceras sorbifolium Bunge oil, which is rich in protein.In this study, the Xanthoceras sorbifolium Bunge seeds meal protein was extracted by the method of ultrasound-assisted alkali extraction and acid precipitation, and the effects of ultrasound amplitude, ultrasound time and pH on the extraction rate of Xanthoceras sorbifolium Bunge seeds meal protein were studied by single factor experiment, and the changes of structural and functional properties of Xanthoceras sorbifolium Bunge seeds meal protein after ultrasound treatment at different time were systematically analyzed.Results showed that the best technology for ultrasound-assisted extraction of Xanthoceras sorbifolium Bunge seeds meal protein was as follows:ultrasound amplitude of 40%, ultrasound time of 9-15 min, pH at 11, and extraction temperature of 45 ℃.Under this condition, the protein extraction rate was the highest, and the main protein molecular weights were 25 and 38 kDa.After 0-12 min ultrasound treatment, the particle size of Xanthoceras sorbifolium Bunge seeds meal protein decreased, its net negative charge increased and hydrophilicity increased.With 15 min ultrasound treatment, the solubility of Xanthoceras sorbifolium Bunge seeds meal protein reached the maximum value of (81.37±1.61)%.Under the same condition, the foaming capacity, emulsifying capacity, water-holding property, and oil-holding property of Xanthoceras sorbifolium Bunge seeds meal protein reached the maximum.Considering the extraction rate and functional properties, 12-15 min ultrasound treatment could be used to extract protein from Xanthoceras sorbifolium Bunge seeds meal, as well as improve its functional properties.The results could provide a basic reference for efficient extraction and property enhancement of Xanthoceras sorbifolium Bunge seeds meal protein.

Key words： Xanthoceras sorbifolium Bunge seeds meal; ultrasound treatment; protein structure; functional properties

Cite this article

LU Qiuling , LI Lang , YANG Min , WEI Yanming . Effect of ultrasound treatment on structural and functional properties of Xanthoceras sorbifolium Bunge seeds meal protein[J]. Food and Fermentation Industries, 2024 , 50(20) : 79 -87 . DOI: 10.13995/j.cnki.11-1802/ts.037719

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