This experiment aimed to improve the extraction methods of antioxidant peptides using membrane separation technology, with a focus on enhancing their application potential in the food industry and health sector, and to support in-depth research into the bioactivity of these antioxidant peptides.Initially, antioxidant peptides were extracted from dried cured beef using the hydrochloric acid extraction method, and their antioxidant capacities at different concentrations (0.2, 0.4, 0.6, 0.8, 1 mg/mL) were tested.It was found that their antioxidant ability increased with concentration.Subsequently, membrane separation technology was employed to purify the antioxidant peptides from beef.Different microporous membranes (PTFE, CA, PVDF, CN-CA, PES, and mixed membranes) were used for separation, and their DPPH free radical and hydroxyl radical scavenging rates were tested.Among them, the CN-CA aqueous mixed fiber microporous membrane showed the best scavenging rates, reaching 77.45% and 51.06%, respectively.The optimized conditions for the preparation of the BTESE/CN-CA composite membrane were 15 g/L solution concentration, 7.5 min heat treatment time, 150 ℃ heat treatment temperature, and 1.25 MPa.Through peptide scoring and half-life analysis, the separated peptide Phe-Asp-Gly-Asp-Phe (FDGDF) was hypothesized to have the strongest activity.Quantum chemical simulations analyzed the binding sites and force fields between FDGDF and superoxide dismutase.This study demonstrates that membrane separation technology can significantly enhance the antioxidant activity of beef peptides, providing valuable insights for research in the fields of food and nutrition.
LI Ruonan
,
XU Shengyu
,
CHEN Wentao
,
XU Xinyu
,
MA Jin
,
ZOU Ping
,
ZHANG Long
,
CHEN Yang
,
ZHANG Yingyang
. Optimization of preparation and activity evaluation of beef antioxidant peptides by membrane separation technique[J]. Food and Fermentation Industries, 2024
, 50(20)
: 171
-180
.
DOI: 10.13995/j.cnki.11-1802/ts.038083
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