Food and Fermentation Industries >

2024 , Vol. 50 >Issue 20: 243 - 251

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037742

Chemical composition analysis and antioxidant activity evaluation of leaf, calyx, and fruit of Physalis peruviana L.

  • LIU Tianyue ,
  • TENG Xiaoyi ,
  • TIAN Yongtao ,
  • YAO Yamei ,
  • WANG Yanbo ,
  • WANG Wenshu
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  • 1(Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), State Ethnic Affairs Commission, Beijing 100081, China)
    2(College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China)

Received date: 2023-10-24

  Revised date: 2023-12-09

  Online published: 2024-11-01

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Abstract

To comprehensively evaluate and analyze the chemical composition and antioxidant activity of Physalis peruviana L., which was the most widely cultivated plant in Physalis genus in China, the contents of total phenols and total flavonoids of extracts from leaf, calyx, and fruit of P.peruviana L.were determined using UV spectrophotometry, the compounds were identified by ultra-high performance liquid chromatography-mass spectrometry and the main compounds were detected.In vitro antioxidant activity was tested using DPPH free radical, ABTS cationic free radical, ·OH free radical scavenging method, and iron ion reduction method.Results indicated that the total phenolic content (8.15 mg GAE/g DW) and total flavonoid content (30.43 mg RT/g DW) of the extract from the leaf of P.peruviana L.were the highest, followed by the extract from the calyx, and the total content of the fruit extract was the lowest.A total of 4 flavonoids, 3 fatty acids, 1 withanolide, 1 dibenzofuran, and 1 hydroxycinnamic acid were identified from the three parts.The types of compounds extracted from the leaf and calyx were abundant, the content of astragalin (492.03 μg/g DW) in the leaf extract was the highest, while the content of hyperoside (162.10 μg/g DW) in the calyx extract was the highest and only ferulic acid-4′-O-glucoside compounds were detected in the fruit extracts (137.37 μg/g DW).The leaf extract had good hydroxyl radical scavenging activity (SC50=0.61 mg/mL), while the calyx extract had the best ability to scavenge DPPH radicals (SC50=0.19 mg/mL), ABTS cationic radicals (0.13 mmol TE/g), and iron ion reduction (0.16 mmol Fe2+/g).The research results have enriched the secondary metabolites data of P.peruviana L., providing a scientific basis for fully utilizing the resources of P.peruviana L.and developing natural antioxidant products.

Key words： Physalis peruviana L.; chemical composition; antioxidant; ultra-high performance liquid chromatography-mass spectrometry

Cite this article

LIU Tianyue , TENG Xiaoyi , TIAN Yongtao , YAO Yamei , WANG Yanbo , WANG Wenshu . Chemical composition analysis and antioxidant activity evaluation of leaf, calyx, and fruit of Physalis peruviana L.[J]. Food and Fermentation Industries, 2024 , 50(20) : 243 -251 . DOI: 10.13995/j.cnki.11-1802/ts.037742

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