Food and Fermentation Industries >

2024 , Vol. 50 >Issue 20: 279 - 285

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038741

Development of an electrochemical immunosensor based on gold nanoparticle/graphene oxide composite film and its detection of marbofloxacin in fresh meat

  • CHEN Xiujin ,
  • WANG Xueqing ,
  • LI Zhaozhou ,
  • WANG Yao ,
  • ZHANG Min ,
  • NIU Huawei ,
  • LIU Hengyan ,
  • AN Biao
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China)
    2(Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Luoyang 471000, China)
    3(National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China)

Received date: 2024-01-28

  Revised date: 2024-04-01

  Online published: 2024-11-01

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Abstract

An electrochemical immunosensor based on glassy carbon electrode-modified gold nanoparticles/graphene oxide composite film was developed and applied to detect marbofloxacin residues in fresh meat.Gold nanoparticles/graphene oxide composite film was synthesized on the electrode surface by an electrodeposition method, and then the graphene oxide of composite film was reduced by an electrochemical method.The antibody was finally immobilized.The modified process of the electrode was characterized by cyclic voltammetry.The electrochemical detection conditions of immunosensor were optimized by differential pulse voltammetry.Under the optimum conditions, marbofloxacin was quantitatively determined.The change of peak current was linearly related to the concentration of marbofloxacin in the range of 0.5-400 ng/mL with a detection limit of 0.05 ng/mL.The detection limits in pork, chicken and beef samples were 0.09, 0.10, and 0.09 μg/kg respectively, and the recovery rates ranged from 82.81% to 101.99%.These results obtained are consistent with that of ultra-high performance liquid chromatography-tandem mass spectrometry.The results showed that the fabricated electrochemical immunosensor could be applied for the detection of marbofloxacin residues in real samples.

Key words： marbofloxacin; gold nanoparticles; graphene oxide; electrochemical immunosensor; fresh meat

Cite this article

CHEN Xiujin , WANG Xueqing , LI Zhaozhou , WANG Yao , ZHANG Min , NIU Huawei , LIU Hengyan , AN Biao . Development of an electrochemical immunosensor based on gold nanoparticle/graphene oxide composite film and its detection of marbofloxacin in fresh meat[J]. Food and Fermentation Industries, 2024 , 50(20) : 279 -285 . DOI: 10.13995/j.cnki.11-1802/ts.038741

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