This study is based on a three-fluid concentric spraying drying technology, using gelatin and gellan gum as wall materials and calcium chloride as crosslinking agent, to encapsulate Lactobacillus plantarum in microcapsules through coacervation, calcium ion cross-linking, or a combination of both.The microcapsules were analyzed using freeze-scanning electron microscopy, solubility tests, and Fourier-transform infrared spectroscopy.The spray-drying survival rate, simulated digestion stability, and storage stability of the probiotic microcapsules were also measured.Results showed that the probiotics were successfully encapsulated within the microcapsules.The addition of gellan gum and calcium ions significantly reduced the solubility and swelling capacity of the gelatin microcapsules.Fourier-transform infrared spectroscopy confirmed the hydrogen bonds and electrostatic interactions between gelatin and pectin, as well as the cross-linking between calcium ions and carboxyl groups.After spray drying, simulated gastric fluid digestion, and 8 weeks of storage at 4 ℃, the microbial activity was 9.71, 8.74, and 9.47 lg CFU/g, respectively.In conclusion, the calcium ion-crosslinked gelatin-gellan gum composite microcapsules could provide excellent protection for probiotics in harsh environments.
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