In order to achieve the high density culture conditions of L.plantarum NCU001929, the optimum culture conditions were determined through the experiments of medium composition, static culture and fermenter technology.The optimal medium for liquid fermentation of L.plantarum NCU001929 contained maltose (34.35 g/L), beef extract (17.40 g/L), yeast extract (18.62 g/L), triammonium citrate (2.80 g/L), bacteriological peptone (10.00 g/L), MnSO4·H2O (0.06 g/L), anhydrous sodium acetate (3.00 g/L), vitamin C (0.02 g/L), Tween-80 (1 mL/L), and dipotassium hydrogen phosphate (2.0 g/L).Suitable culture conditions were 2% inoculation amount, pH 7 and the culture temperature 30 ℃, with the viable bacteria number of 6.40×109 CFU/mL, which is 4.81 times that of initial MRS medium.Additionally the optimal process parameters for fermentation tanks were stir speed 150 r/min,incubate with sterile air, constant pH of 7 and neuralizing agent 25% NaOH, with the viable bacteria number of 1.32×1010 CFU/mL, which is 9.92 times that of initial MRS medium.In conclusion, a preparation method of red sour soup direct vet set was established, which provides a technical support for the industrial production of Guizhou red sour soup.
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