This study aimed to investigate the impact of different solvent extraction methods on the quality of Antarctic krill oil.The extraction efficiency of total lipid, phosphatidylcholine (PC), lysophosphatidylcholine (LPC), as well as the levels of eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) were selected as the investigation parameters.Furthermore, the molecular species composition of PC/LPC in the extracted Antarctic krill oil was examined and analyzed using hydrophilic interaction chromatography-tandem mass spectrometry technology.Consequently, the effects of various solvents, including 95% ethanol, n-hexane, isopropyl alcohol, ethyl acetate, isopropyl alcohol/n-hexane (1∶1, v∶v), ethyl acetate/n-hexane (1∶1, v∶v), and Folch solvent (trichloroethane/methanol, 2∶1, v∶v), on the quality of Antarctic krill oil were examined.Results showed that 95% ethanol and isopropyl alcohol/n-hexane exhibited higher extraction efficiency, as well as higher EPA and DHA contents (20.05% and 20.42%, respectively).Conversely, the extraction efficiency and EPA/DHA levels were lower when n-hexane and ethyl acetate were employed.In addition, 95% ethanol, ethyl acetate/n-hexane, and isopropyl alcohol/n-hexane demonstrated greater extraction efficiency for PC/LPC, while the efficiency of ethyl acetate, isopropyl alcohol, and n-hexane was comparatively lower.Furthermore, n-hexane, ethyl acetate/n-hexane, and isopropyl alcohol yielded higher proportions of PC 22∶6/22∶6, PC 20∶5/22∶6, and PC 20∶5/20∶5.Conversely, LPC extracted with isopropyl alcohol/n-hexane, Folch solvent, and 95% ethanol contained a higher proportion of PC 0∶0/20∶5 and PC 0∶0/22∶6.In conclusion, the results suggested that 95% ethanol and isopropyl alcohol/n-hexane exhibited notable enrichment effects on EPA/DHA and PC, making them the most suitable solvents for the extraction of Antarctic krill oil.
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