Food and Fermentation Industries >

2024 , Vol. 50 >Issue 24: 169 - 175

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038426

Safety and probiotic characteristics of Limosilactobacillus fermentum FUA033 based on complete genome analysis

  • MU Shuting ,
  • HUA Ziyan ,
  • ZHAO Shuangshuang ,
  • FANG Yaowei ,
  • YANG Guang ,
  • HOU Xiaoyue ,
  • GONG Yanyan ,
  • GUI Jiajin ,
  • LIU Shu
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  • 1(Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China)
    2(School of Marine Food and Bioengineering Jiangsu Ocean University, Lianyungang 222005, China)
    3(Lianyungang Food and Drug Inspection and Testing Center, Lianyungang 222005, China)

Received date: 2024-01-03

  Revised date: 2024-02-07

  Online published: 2024-12-30

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Abstract

Limosilactobacillus fermentum FUA033, a strain capable of converting ellagic acid to urolithin A in vitro, has the potential to be developed as a next-generation probiotic.In the present study, the genome of the strain was sequenced with the PacBio Sequel and Illumina platform to have a comprehensive understanding of the genetic architecture of L.fermentum FUA033.The whole genome sequence was analyzed to evaluate the safety and probiotic potential of the strain.The chromosome size of this strain was 2 150 545 bp with a GC content of 51.34% and 2 215 protein-coding genes.No plasmid was identified.VFDB database and ARDB database annotation results showed that L.fermentum FUA033 contained 81 virulence genes and 9 resistance genes.Due to the absence of plasmids and transposons, it was tentatively concluded that the virulence factors and resistance genes would not transfer.19 acid tolerance genes (arcA, argH, napA, etc.), 16 bile salt tolerance genes (ldhA、ykpA、pstB1, etc.), 11 antioxidant genes (msrA, Trxs, ahpC, etc.), and 3 cell adhesion genes were mined from the genome sequence of L.fermentum FUA033.The results preliminarily revealed the safety and probiotic properties of L.fermentum FUA033 and laid a theoretical foundation for the development and further application of the strain.

Key words： Limosilactobacillus fermentum; urolithin A; genome sequencing; safety; probiotics

Cite this article

MU Shuting , HUA Ziyan , ZHAO Shuangshuang , FANG Yaowei , YANG Guang , HOU Xiaoyue , GONG Yanyan , GUI Jiajin , LIU Shu . Safety and probiotic characteristics of Limosilactobacillus fermentum FUA033 based on complete genome analysis[J]. Food and Fermentation Industries, 2024 , 50(24) : 169 -175 . DOI: 10.13995/j.cnki.11-1802/ts.038426

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