Analysis of bitter compounds in red huajiao (Zanthoxylum bungeanum Maxim.) peels from different regions

  • WANG Lu ,
  • LI Aijun ,
  • DUAN Ping ,
  • PENG Xiaowei ,
  • KAN Jianquan
Expand
  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture and Rural Affairs, Chongqing 400715, China)
    3(Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, China)
    4(Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)

Received date: 2024-01-09

  Revised date: 2024-02-04

  Online published: 2024-12-30

Abstract

Huajiao (Zanthoxylum bungeanum), known for its strong pungency and unique aroma, is widely employed in regional cuisines and food processing.However, its development is hindered by its obvious bitter taste.Red huajiao (Zanthoxylum bungeanum Maxim.) has extensive cultivation and production.To compare the bitterness difference in red huajiao peels from different regions, this study focused on 20 distinct regions of red huajiao peels and used HPLC and amino acid autoanalyzer to analyze bitter compounds contents.Results showed that bitter substances were distributed in the red huajiao peels from 20 regions, and the content was different.Quercetin and arbutin were the main bitter substances in most areas, with the contents of 0.127-1.200 mg/g and 0.451-1.918 mg/g, respectively.Hierarchical cluster analysis (HCA) categorized 20 regions into five distinct classes based on bitter compounds composition and contents.Isoquercitrin, quercetin, and isorhamnetin were important bitter compounds influencing the classification of red huajiao peels into different clusters, and they were screened through combined partial least squares discrimination anlysis (PLS-DA) with variable importance in project (VIP).This study establishes a foundational data set for quality control purposes of red huajiao peels.

Cite this article

WANG Lu , LI Aijun , DUAN Ping , PENG Xiaowei , KAN Jianquan . Analysis of bitter compounds in red huajiao (Zanthoxylum bungeanum Maxim.) peels from different regions[J]. Food and Fermentation Industries, 2024 , 50(24) : 298 -303 . DOI: 10.13995/j.cnki.11-1802/ts.038533

References

[1] 胡晴文, 彭郁, 李茉, 等.花椒油和花椒籽油提取技术研究进展[J].中国油脂, 2024, 49(1):16-21.
HU Q W, PENG Y, LI M, et al.Research progress on extraction process of Zanthoxylum bungeanum oil and Zanthoxylum bungeanum seed oil[J].China Oils and Fats, 2024, 49(1):16-21.
[2] KE J X, CHENG J X, LUO Q Y, et al.Identification of two bitter components in Zanthoxylum bungeanum Maxim.and exploration of their bitter taste mechanism through receptor hTAS2R14[J].Food Chemistry, 2021,338:127816.
[3] 杨青青, 王智荣, 彭林, 等.基于代谢组学分析两种产地青花椒中非挥发性成分的差异[J].食品与发酵工业, 2021,47(12):216-223.
YANG Q Q, WANG Z R, PENG L, et al.Metabolomic analysis of non-volatile chemical components in Zanthoxylum schinifolium Sieb.et Zucc from two origins[J].Food and Fermentation Industries, 2021,47(12):216-223.
[4] 梅小飞. 红花椒果皮苦味形成的关键苦味物质研究[D].重庆:西南大学, 2020.
MEI X F.Identification of key bitter compounds in the formation of bitter taste of red huajiao (Zahthoxylum simulans Hance) peel[D].Chongqing:Southwest University, 2020.
[5] YANG Q Q, WANG Z R, CHEN X H, et al.Evaluation of bitter compounds in Zanthoxylum schinifolium Sieb.et Zucc by instrumental and sensory analyses[J].Food Chemistry, 2022,390:133180.
[6] HUANG S, WANG L, WANG Z R, et al.Multiomics strategy reveals the accumulation and biosynthesis of bitter components in Zanthoxylum schinifolium Sieb.et Zucc[J].Food Research International, 2022,162:111964.
[7] 姚佳, 蒲彪.青花椒的研究进展[J].中国调味品, 2010,35(6):35-39.
YAO J, PU B.Research progress on Zanthoxylum schinifolium sieb.et Zucc[J].China Condiment, 2010,35(6):35-39.
[8] 李宏梁, 薛婷.花椒果皮的研究进展[J].中国调味品, 2014,39(1):124-128;135.
LI H L, XUE T.Research progress of pericarpium zanthoxyli peel[J].China Condiment, 2014,39(1):124-128;135.
[9] 罗凯, 朱琳, 阚建全, 等.不同产地青花椒和红花椒挥发油的比较研究[J].食品工业科技, 2012,33(18):103-106.
LUO K, ZHU L, KAN J Q, et al.Comparative study of the essential oil content of Zanthoxylum Schinifolium Sieb.et Zucc and Zanthosylum Bungeanum Maxim.from different producing areas[J].Science and Technology of Food Industry, 2012,33(18):103-106.
[10] 阚建全, 陈科伟, 任廷远, 等.花椒麻味物质的生理作用研究进展[J].食品科学技术学报, 2018,36(1):11-17;44.
KAN J Q, CHEN K W, REN T Y, et al.Review on physiological function of alkylamide compounds from Zanthoxylum bungeanum[J].Journal of Food Science and Technology, 2018,36(1):11-17;44.
[11] 高露, 赵镭, 史波林, 等.气相色谱-质谱联用结合气味活度值分析红花椒油的关键香气物质特征[J].食品与发酵工业, 2023,49(22):295-301.
GAO L, ZHAO L, SHI B L, et al.GC-MS combined with odor activity value to analyze key aroma substance characteristics of red Chinese pepper oil[J].Food and Fermentation Industries, 2023,49(22):295-301.
[12] 赵奕彭. 软枣猕猴桃中生物碱的提取工艺优化、分离及抗氧化活性研究[D].沈阳:沈阳农业大学, 2019.
ZHAO Y P.Optimum extraction isolation of Actinidia arguta alkaloids, and antioxidant activity[D].Shenyang:Shenyang Agricultural University, 2019.
[13] JIANG Z M, WANG L J, GAO Z, et al.Green and efficient extraction of different types of bioactive alkaloids using deep eutectic solvents[J].Microchemical Journal, 2019,145:345-353.
[14] 陈彦林. 不同干制方式龙眼果肉主要活性物质的比较[D].武汉:华中农业大学, 2014.
CHEN Y L.Comparison of main active substances in longan pulp by different drying methods[D].Wuhan:Huazhong Agricultural University, 2014.
[15] LIOE H N, APRIYANTONO A, TAKARA K, et al.Umami taste enhancement of MSG/NaCl mixtures by subthreshold L-α-aromatic amino acids[J].Journal of Food Science, 2005,70(7):S401-S405.
[16] YAN J N, TONG H R.An overview of bitter compounds in foodstuffs:Classifications, evaluation methods for sensory contribution, separation and identification techniques, and mechanism of bitter taste transduction[J].Comprehensive Reviews in Food Science and Food Safety, 2023,22(1):187-232.
[17] SARROU E, CHATZOPOULOU P, DIMASSI-THERIOU K, et al.Effect of melatonin, salicylic acid and gibberellic acid on leaf essential oil and other secondary metabolites of bitter orange young seedlings[J].Journal of Essential Oil Research, 2015,27(6):487-496.
[18] ISMAIL F, VAISEY-GENSER M, FYFE B.Bitterness and astringency of sinapine and its components[J].Journal of Food Science, 1981,46(4):1 241-1 244.
[19] 陈锦平, 潘丽萍, 邢颖, 等.外源茉莉酸对小白菜耐硒性及硒积累的作用研究[J].作物杂志, 2023(6):160-166.
CHEN J P, PAN L P, XING Y, et al.Study on the effects of exogenous jasmonic acid on selenium tolerance and selenium accumulation in pak choi(Brassica chinensis L.)[J].Crops, 2023(6):160-166.
[20] 潘明凤, 魏屹.RP-HPLC法测定野花椒中木兰花碱的含量[J].安徽农业科学, 2012,40(31):15416-15417.
PAN M F, WEI Y.Determination of magnoflorine in Zanthoxylum simullans hance by RP-HPLC[J].Journal of Anhui Agricultural Sciences, 2012,40(31):15 416-15 417.
[21] 周瑞, 项昌培, 张晶晶, 等.黄连化学成分及小檗碱药理作用研究进展[J].中国中药杂志, 2020,45(19):4561-4573.
ZHOU R, XIANG C P, ZHANG J J, et al.Research progress on chemical compositions of Coptidis Rhizoma and pharmacological effects of berberine[J].China Journal of Chinese Materia Medica, 2020,45(19):4561-4573.
[22] ZHANG Y R, FU J M, ZHOU Q Y, et al.Metabolite profiling and transcriptome analysis revealed the conserved transcriptional regulation mechanism of caffeine biosynthesis in tea and coffee plants[J].Journal of Agricultural and Food Chemistry, 2022,70(10):3239-3251.
[23] 刘朝茂, 杨雨雯, 李萍, 等.花椒病虫害研究进展[J].中国森林病虫, 2020,39(2):28-35.
LIU C M, YANG Y W, LI P, et al.Research progress on diseases and insect pests of Zanthoxylum bungeanum[J].Forest Pest and Disease Mar., 2020,39(2):28-35.
[24] CHUNG K T, WONG T Y, WEI C I, et al.Tannins and human health:A review[J].Critical Reviews in Food Science and Nutrition, 1998,38(6):421-464.
[25] FENG X Y, WANG H W, WANG Z R, et al.Discrimination and characterization of the volatile organic compounds in eight kinds of Huajiao with geographical indication of China using electronic nose, HS-GC-IMS and HS-SPME-GC-MS[J].Food Chemistry, 2022,375:131671.
Outlines

/

〈 〉